Protective Effects of Exogenous Melatonin on Cisplatin- Induced Acute Nephrotoxicity in Rats

Abstract

This study was planned to investigate the consequences of exogenous administration ofmelatonin on the amelioration of some biochemical variables in cisplatin (CP)-inducedacute nephrotoxicity in rats. Twenty-four male rats weighing 300-350g were used.Housing of the animals and the experiment were achieved and the rats were -housed inthe Department of Biology /College of Science/ Sulaimani University. The Animals weredivided into three groups (n=5); control group (receiving prepared standard chow for ratsand water ad libitum); model group(The animals administered a single intraperitoneal(i.p) dose of CP (8 mg/kg BW.); and the third group in addition to CP injection theanimals were supplemented with melatonin (180 mg/kg food) at the same day of CP-injection for five days. Finally, the blood specimen was taken using cardiac puncture.The studied parameters include Malondialdehyde (MDA), serum creatinine, serum urea,serum total bilirubin, serum albumin, serum electrolytes (Ca+2, Na+ and K+), as well assome hematological parameters (WBC, RBC, HCT, HGB). Our results showedsignificant (P<0.05) elevation in the serum level of MDA,, creatinine, urea and totalbilirubin in model group. On the other hand, the WBC count was also increasedsignificantly (P<0.01), whereas there was no significant alterations in the level ofcalcium, sodium, and potassium. The outcome of our study indicated that the melatoninadministration in injected rats for five days significantly (P<0.01) improved the elevatedserum levels of MDA, creatinine, urea, total bilirubin and WBC count. In conclusion,this study confirmed that melatonin has protective effects of ameliorating thenephrotoxicity induced by cisplatin in rat.