Protective effects of edible insect protein extracts from Protaetia brevitarsis against H2O2-induced oxidative stress in mouse C2C12 myoblast cells

Lee, Jae Hoon; Kim, Tae‐Kyung; Kang, Min‐Cheol; Kim, Bum-Keun; Choi, Yun‐Sang

doi:10.1016/j.fbio.2023.102396

Cited by 16 publications

(14 citation statements)

References 43 publications

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“…A previous study has shown that protein hydrates of P. brevitarsis larvae reduce H 2 O 2 -induced oxidative stress in AML12 cells (hepatocytes) by regulating the Nrf2/HO-1 signaling pathway [ 33 ]. Moreover, protein extracts of P. brevitarsis larvae showed reduced ROS production against H 2 O 2 -induced oxidative stress in C2C12 myoblast [ 48 ]. Also in a prior study, supplementation with BCAAs in-creased the mRNA levels of glutathione peroxidase 1 (GPX1), catalase, and SOD in the liver tissue of alcohol-induced rats [ 49 ].…”

Section: Discussionmentioning

confidence: 99%

Fermented Protaetia brevitarsis Larvae Ameliorates Chronic Ethanol-Induced Hepatotoxicity in Mice via AMPK and TLR-4/TGF-β1 Pathways

Lee,

Kim,

et al. 2023

J. Microbiol. Biotechnol.

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This study evaluated the hepatoprotective effect of fermented Protaetia brevitarsis larvae (FPB) in ethanol-induced liver injury mice. As a result of amino acids in FPB, 18 types of amino acids including essential amino acids were identified. In the results of in vitro tests, FPB increased alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH) activities. In addition, FPB treatment increased cell viability on ethanol- and H 2 O 2 -induced HepG2 cells. FPB ameliorated serum biomarkers related to hepatoxicity including glutamic oxaloacetic transaminase, glutamine pyruvic transaminase, total bilirubin, and lactate dehydrogenase and lipid metabolism including triglyceride, total cholesterol, high-density lipoprotein cholesterol, and low-density lipoprotein cholesterol. Also, FPB controlled ethanol metabolism enzymes by regulating the protein expression levels of ADH, ALDH, and cytochrome P450 2E1 in liver tissue. FPB protected hepatic oxidative stress by improving malondialdehyde content, reduced glutathione, and superoxide dismutase levels. In addition, FPB reversed mitochondrial dysfunction by regulating reactive oxygen species production, mitochondrial membrane potential, and ATP levels. FPB protected ethanol-induced apoptosis, fatty liver, and hepatic inflammation through p-AMP-activated protein kinase and TLR-4/NF-κB signaling pathways. Furthermore, FPB prevented hepatic fibrosis by decreasing TGF-β1/Smad pathway. In summary, these results suggest that FPB might be a potential prophylactic agent for the treatment of alcoholic liver disease via preventing liver injury such as fatty liver, hepatic inflammation due to chronic ethanol-induced oxidative stress.

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Section: Discussionmentioning

confidence: 99%

Fermented Protaetia brevitarsis Larvae Ameliorates Chronic Ethanol-Induced Hepatotoxicity in Mice via AMPK and TLR-4/TGF-β1 Pathways

Lee,

Kim,

et al. 2023

J. Microbiol. Biotechnol.

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“… Lee et al (2023) have previously reported that two types of hydrolysates of the edible insect Protaetia brevitarsis (100 μg/mL of myofibril protein and 200 μg/mL of sarcoplasmic protein hydrolysates) restore the viability of H 2 O 2 -treated C2C12 cells by approximately 73.28% and 76.38%, respectively; these hydrolysates are mainly composed of 150-, 75-, 37-, and 10-kDa peptides. Sivakumar et al (2014) reported that 50.4 μM of a 1202.56-Da peptide from bovine muscle increases the viability of H 2 O 2 -treated C2C12 cells by approximately two times.…”

Section: Resultsmentioning

confidence: 97%

Effects of Horse Meat Hydrolysate on Oxidative Stress, Proinflammatory Cytokines, and the Ubiquitin-Proteasomal System of C2C12 Cells

Lee,

Kim,

et al. 2024

Food Sci Anim Resour

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Sarcopenia, the age-related muscle atrophy, is a serious concern as it is associated with frailty, reduced physical functions, and increased mortality risk. Protein supplementation is essential for preserving muscle mass, and horse meat can be an excellent source of proteins. Since sarcopenia occurs under conditions of oxidative stress, this study aimed to investigate the potential anti-muscle atrophy effect of horse meat hydrolysate using C2C12 cells. A horse meat hydrolysate less than 3 kDa (A4<3kDa) significantly increased the viability of C2C12 myoblasts against H 2 O 2 -induced cytotoxicity. Exposure of C2C12 myoblasts to lipopolysaccharide led to an elevation of cellular reactive oxygen species levels and mRNA expression of proinflammatory cytokines, including tumor necrosis factor-α and interleukin 6, and these effects were attenuated by A4<3kDa treatment. Additionally, A4<3kDa activated protein synthesis-related proteins through the protein kinase B/mechanistic target of rapamycin pathway, while decreasing the expression of activity and degradation-related proteins, such as Forkhead box O3, muscle RING finger protein-1, and Atrogin-1 in dexamethasone-treated C2C12 myotubes. Therefore, the natural material A4<3kDa has the potential ofprotecting against muscle atrophy, while further in vivo study is needed.

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“…Consequently, chronic consumption of ethanol can lead to mitochondrial dysfunction characterized by accumulation of ROS, production of ATP, and reduction of MMP within mitochondria. However, a previous study reported that treatment with protein extracts from P. brevitarsis resulted in the improvement of mitochondrial function via H 2 O 2 -induced reduction of MMP in C2C12 myoblast cells [ 11 ]. PUFAs are known to be involved in homeostasis of mitochondrial calcium, generation of ROS, and mitochondrial apoptosis, which can be beneficial in the prevention of diseases such as AD, myocardial infarction, diabetes, and obesity [ 36 ].…”

Section: Discussionmentioning

confidence: 99%

“…Indeed, consumption of alcohol can induce dementia through apoptosis related to neuronal loss [ 41 ]. Treatment with P. brevitarsis protein extract has been reported to decrease the BAX/BCL-2 ratio, thereby exerting a protective effect against cytotoxicity in H 2 O 2 -induced C2C12 cells [ 11 ]. In addition, the results of pretreatment with P. brevitarsis larvae showed a reduction in the number of apoptotic nuclei in radiation-induced rat testicular germ cells [ 29 ].…”

Section: Discussionmentioning

confidence: 99%

“…Insects are a natural resource that have been utilized for various purposes as ingredients in food and medicine since ancient times [ 10 ]. Due to explosive population growth worldwide, edible insects, which are considered nutritionally excellent food materials, have been regarded as a next-generation alternative food source [ 10 , 11 ]. However, despite the conducting of nutritional and bio-functional research using various edible insects, industrialization has not been attempted due to consumer aversion [ 6 , 10 ].…”

Section: Introductionmentioning

confidence: 99%

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Fermented Protaetia brevitarsis Larvae Improves Neurotoxicity in Chronic Ethanol-Induced-Dementia Mice via Suppressing AKT and NF-κB Signaling Pathway

Lee,

Kim,

et al. 2024

IJMS

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This study was investigated to examine the neuroprotective effect of fermented Protaetia brevitarsis larvae (FPB) in ethanol-induced-dementia mice. Consumption of FPB by mice resulted in improved memory dysfunction in the Y-maze, passive avoidance, and Morris water maze tests. FPB significantly decreased oxidative stress by regulating levels of malondialdehyde (MDA), superoxide dismutase (SOD), and reduced glutathione (GSH) in brain tissues. In addition, FPB restored cerebral mitochondrial dysfunction by modulating levels of reactive oxygen species (ROS), mitochondrial membrane potential (MMP), and ATP. In addition, FPB enhanced the cholinergic system via the regulation of acetylcholine (ACh) content, acetylcholinesterase (AChE) activity, and expressions of AChE and choline acetyltransferase (ChAT) in brain tissues. FPB ameliorated neuronal apoptosis through modulation of the protein kinase B (AKT)/B-cell lymphoma (BCL)-2 signaling pathway. Also, FPB improved inflammation response by down-regulating the toll-like receptor (TLR)-4/nuclear factor (NF)-κB pathway. Additionally, FPB ameliorated synaptic plasticity via the increase of the expressions of synaptophysin (SYP), postsynaptic density protein (PSD)-95, and growth-associated protein (GAP)-43. Treatment with FPB also reinforced the blood–brain barrier by increasing tight junctions including zonula occludens (ZO)-1, occludin, and claudin-1. In conclusion, these results show that FPB can improve cognitive impairment via AKT/NF-κB pathways in ethanol-induced-dementia mice.

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Protective effects of edible insect protein extracts from Protaetia brevitarsis against H2O2-induced oxidative stress in mouse C2C12 myoblast cells

Cited by 16 publications

References 43 publications

Fermented Protaetia brevitarsis Larvae Ameliorates Chronic Ethanol-Induced Hepatotoxicity in Mice via AMPK and TLR-4/TGF-β1 Pathways

Fermented Protaetia brevitarsis Larvae Ameliorates Chronic Ethanol-Induced Hepatotoxicity in Mice via AMPK and TLR-4/TGF-β1 Pathways

Effects of Horse Meat Hydrolysate on Oxidative Stress, Proinflammatory Cytokines, and the Ubiquitin-Proteasomal System of C2C12 Cells

Fermented Protaetia brevitarsis Larvae Improves Neurotoxicity in Chronic Ethanol-Induced-Dementia Mice via Suppressing AKT and NF-κB Signaling Pathway

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