Protective effects of doxycycline in ischemia/reperfusion injury on kidney

Küçük, Ayşegül; Kabadere, Selda; Tosun, Murat; Köken, Tülay; Kinaci, M. Kenan; Işikli, Burhanettin; Erkasap, Nilüfer

doi:10.1007/bf03179069

Cited by 33 publications

(28 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…I/R induces oxidative stress in the kidneys, which causes the enhancement of the reactive oxygen products and the disappearance of the antioxidant defense system (8). In the present study, I/R injury was created in the renal tissue of rats and it was found that the renal damage created by I/R was significantly decreased by quercetin treatment.…”

Section: Discussionsupporting

confidence: 50%

See 1 more Smart Citation

Effects of quercetin on apoptosis, NF-κB and NOS gene expression in renal ischemia/reperfusion injury

Kinaci

Erkasap

Küçük

et al. 2011

Experimental and Therapeutic Medicine

View full text Add to dashboard Cite

Abstract. The aim of this study was to investigate the effects of quercetin on nitric oxide synthase (NOS), nuclear factor-κB (NF-κB) and apoptosis in renal ischemia/reperfusion (I/R) injury in rats. A total of 42 Sprague-Dawley rats were divided into three groups. The control, I/R and I/R+quercetin (I/R+Q) groups were treated with quercetin (50 mg/kg intraperitoneal) 1 h prior to the induction of ischemia. Tissue malondialdehyde (MDA) and glutathione (GSH) levels were determined by high-performance liquid chromatography (HPLC). p53, endothelial NOS (eNOS) and NF-κB expression were assessed immunohistochemically, and apoptosis assesment was performed using terminal deoxy nucleotidyl transferase dUTP nick end-labeling (TUNEL) assay. The mRNA levels of inducible NOS (iNOS) in renal tissue were determined by realtime polymerase chain reaction (RT-PCR). MDA levels were significantly decreased in the quercetin group compared to the I/R group. However, GSH levels were significantly increased with quercetin treatment in the I/R group. Histological results, the number of apoptotic and p53-positive cells, NF-κB and eNOS expression levels were significantly decreased in the quercetin treatment group compared to the I/R group. iNOS gene expression increased in the I/R group, but no significant difference was found between the I/R and quercetin treatment groups. Therefore, quercetin not only has antioxidant and antiapoptotic activities, but also has an inhibitory effect on eNOS and NF-κB for renal tissue protection during I/R injury in rats. Therefore, quercetin may be a promising renoprotective therapeutic agent.

show abstract

Section: Discussionsupporting

confidence: 50%

“…Apoptosis is a form of programmed cell death, and in this way cells that have been damaged in the organism are removed without harming their environment (8,9). p53 is a tumor suppressor gene that plays an important role in cell cycle control and apoptosis.…”

Section: Introductionmentioning

confidence: 99%

Effects of quercetin on apoptosis, NF-κB and NOS gene expression in renal ischemia/reperfusion injury

Kinaci

Erkasap

Küçük

et al. 2011

Experimental and Therapeutic Medicine

View full text Add to dashboard Cite

show abstract

“…The function and structure of TECs depend on the establishment and maintenance of surface membrane polarity. Ischemic acute renal failure has been shown to cause apical membrane disruption, collapse of microvilli, and loss of cell polarity in proximal tubules [2,3,4,5]. A growing body of literature has demonstrated that the actin cytoskeleton plays a role in the regulation of several signaling pathways.…”

Section: Introductionmentioning

confidence: 99%

Kidney Protection against Ischemia/Reperfusion Injury by Myofibrillogenesis Regulator-1

Wang

Tao²,

Ding³

et al. 2014

Am J Nephrol

View full text Add to dashboard Cite

Background/Aims: Ischemia/reperfusion (I/R) injury is characterized by cytoskeletal reorganization and loss of polarity in proximal tubule epithelial cells. Previously, we showed that myofibrillogenesis regulator (MR)-1 promoted actin organization in cardiomyocytes. MR-1 is also expressed in the kidney. Methods: In this study, we investigated MR-1 expression in acute renal failure induced by I/R in Sprague-Dawley rats. We determined the MR-1 expression and the ratio of fibrous actin (F-actin) to globular actin (G-actin). HK-2 cells were treated with or without hypoxia/reoxygenation (H/R), and MR-1 levels were increased by adenoviral overexpression or silenced by RNA interference. Results: I/R and H/R resulted in cellular injury and decreases of MR-1, the F-/G-actin ratio, and myosin light chain (MLC)-2. MR-1 overexpression attenuated H/R-induced cell injury and loss of surface membrane polarity of actin. MR-1 overexpression also increased the expression and phosphorylation of MLC-2 and MLC kinase, which were decreased in MR-1-silenced and H/R-treated cells. Conclusion: Together, these data show that MR-1 promoted actin polarity on the membrane surface and protected HK-2 cells from H/R injury. The mechanism might involve the rapid organization of F-actin through the upregulation and phosphorylation of MLC-2.

show abstract

“…El uso de doxiciclina, tanto en animales infectados por E. canis como en animales sanos, demostró disminuir progresivamente la concentración de creatinina sérica que sugiere un efecto nefroprotector (5). Algunos estudios han descrito, que a altas dosis el uso de doxiciclina puede bajar la tasa de filtración glomerular causando estados de isquemia en la arteriola eferente que puede llegar a inducir el incremento en los valores de creatinina sérica, una de las teorías es la reducción en el estrés oxidativo, disminuyendo los niveles de citoquinas e inhibición de la actividad de metaloproteinasas de matriz (5,30). Se ha determinado en diferentes estudios las propiedades antiinflamatorias e inmunomoduladoras de la doxiciclina asociadas a estos efectos (31,32).…”

Section: Ehrlichia Canis and The Effects Of Doxycycline In The Treatmentunclassified

“…One of the theories is the reduction of the oxidative stress, decreasing cytokine levels and inhibiting the activity of matrix metalloproteinases (5,30). The anti-inflammatory and immunomodulatory properties of doxycycline associated with these effects have been determined in different studies (31,32).…”

Section: Sistema Inmunementioning

confidence: 99%

Farmacocinética y efectos adversos de la doxiciclina en el tratamiento de la Ehrlichiosis: fundamentos teóricos para ensayos clínicos en caninos

Molina

et al. 2017

Rev MVZ Córdoba

View full text Add to dashboard Cite

Ehrlichia canis is the causative bacterium of canine monocytic ehrlichiosis (CME), a disease of global importance in veterinary and human medicine. Several studies have addressed the therapeutic efficacy of tetracycline hydrochloride and doxycycline hyclate (HD) for the treatment of CME, however the results are still controversial. Doxycycline is the treatment of choice for canine monocytic ehrlichiosis (CME), a well characterized disease that can serve as a model for research in diseases of the Rickettsial order and tick-borne zoonoses. Although the pharmacokinetics and efficacy of the treatment in the acute and subclinical CME phases have been known for decades, some results also indicate that Ehrlichia canis may persist in clinically normal dogs, even after an extensive treatment regimen. The purpose of this review is to (major delve into/further investigate) the pharmacokinetics and side effects of doxycycline in the treatment of canine ehrlichiosis.Keywords: Blood chemistry, Ehrlichia canis, hematology, pharmacokinetics, tetracycline (Source: CAB). RESUMENEhrlichia canis es la bacteria causante de la Ehrlichiosis monocítica canina (EMC), una enfermedad de importancia global en la medicina veterinaria y humana. Varios estudios han abordado la eficacia terapéutica de hidrocloruro de tetraciclina e hiclato de doxiciclina (HD) para el tratamiento de la EMC, sin embargo los resultados obtenidos siguen siendo controvertidos. La doxiciclina es el tratamiento de elección para la EMC, enfermedad bien caracterizada que puede servir como modelo para las investigaciones en el estudio de las enfermedades del orden de los Rickettsiales y las zoonosis transmitidas por garrapatas. Aunque desde décadas atrás se conoce su farmacocinética y la eficacia en el tratamiento de la EMC en fase aguda y subclínica, algunos resultados también indican que Ehrlichia canis puede persistir en perros clínicamente normales, incluso después de un amplio régimen de tratamiento. El propósito de esta revisión es hacer un acercamiento referente a la farmacocinética y los efectos adversos de la doxiciclina en el tratamiento de la EMC.

show abstract

Protective effects of doxycycline in ischemia/reperfusion injury on kidney

Cited by 33 publications

References 30 publications

Effects of quercetin on apoptosis, NF-κB and NOS gene expression in renal ischemia/reperfusion injury

Effects of quercetin on apoptosis, NF-κB and NOS gene expression in renal ischemia/reperfusion injury

Kidney Protection against Ischemia/Reperfusion Injury by Myofibrillogenesis Regulator-1

Farmacocinética y efectos adversos de la doxiciclina en el tratamiento de la Ehrlichiosis: fundamentos teóricos para ensayos clínicos en caninos

Contact Info

Product

Resources

About