Protective effects of curcumin on methylglyoxal-induced oxidative DNA damage and cell injury in human mononuclear cells

Abstract

Aim: To examine the effect of curcumin on oxidative DNA damage and cell apoptosis and injury caused by the reaction of methylglyoxal(MG) with amino acids. Methods: We used DNA strand breaks to examine the effect of curcumin on oxidative DNA damage. In addition, reactive oxygen species(ROS) formation occurs in MG‐treated mononuclear cells, so the effect of curcumin on ROS generation was measured using 2′,7′‐dichlorofluorescin diacetate(DCF‐DA) as the detection reagent. Moreover, the impact effects of curcumin on MG‐induced cell apoptosis and ROS injury were analyzed by TUNEL and ELISA assay. The collagen I attachment ability of mononuclear cells was examined by trypan blue staining. Results: Our results revealed that curcumin prevented MG/lysine‐induced oxidative stress and DNA damage. Curcumin also inhibited MG‐induced apoptosis and generation of ROS in mononuclear cells. MG‐treated mononuclear cells displayed a lower degree of attachment to collagen (the major component of the vessel wall subendothelium), whereas cells pretreated with curcumin before MG treatment exhibited restored affinities for collagen. Conclusion: These results demonstrated that oxidative stress plays a role in MG‐induced cell injury and alterations in attachment ability, and that curcumin blocks these effects by virtue of its antioxidant properties.

Protective effect of curcumin against arsenic-induced apoptosis in murine splenocytesin vitro

“…In vitro studies have also reported Cur-related modulation of arsenic-induced genotoxicity in human lymphocytes (Sinha et al, 2009). Along these lines, Cur was also found to be effective in preventing methylglyoxal (MG)-induced oxidative DNA damage, cell injury, apoptosis, and generation of ROS in mononuclear cells (Chan and Wu, 2006).…”

Effect of Curcumin Against Oxidation of Biomolecules by Hydroxyl Radicals

“…DNA fragmentation has been utilized as a characteristic feature of apoptosis [50]. conducted by Ryu Ar et al, [53], Madkour NK [54] Zhu YG et al, [55], Chan WH et al, [56], who stated that curcumin can also inhibit the oxidative DNA damage by Chrysotile, Lambda cyhalothrin, tertbutyl hydroperoxide (t-BHP) and Methylglyoxal respectively.…”

Concurrent Administration of Curcumin Mitigates Arsenic - and Chlorpyrifos - induced Apoptosis in Rat Thymocytes

“…Curcumin is a potent scavenger of a variety of reactive oxygen species including superoxide anion radicals, hydroxyl radicals and inhibit lipid peroxidation and effectively block thiol depletion (Chattopadhyay et al, 2005). Furthermore, Curcumin was also found to be effective in preventing methylglyoxal (MG)-induced oxidative DNA damage, cell injury, apoptosis, and generation of ROS in mononuclear cells (Chan and Wu, 2006) .…”