Protective effects of curcumin on kidney tissue in ischemia-reperfusion injury in rats

Abstract

Aim: Kidney damage caused by ischemia-reperfusion (IR) is a serious clinical problem. Many studies have emphasized the antioxidant and anti-inflammatory properties of Curcumin. Material and Methods: Wistar-Albino male rats were divided into three groups. The sham group was the group in which only laparotomy was performed, the IR group was the group in which the infrarenal aorta was clamped after laparotomy and ischemia-reperfusion was created, and the IR+ curcumin group was the group in which intraperitoneal curcumin was given 1 hour before the procedure and the same procedures were repeated with the IR group. After creating the IR, blood and kidney tissue were taken from the rats and biochemical and histopathological examinations were performed. Results: Potassium (p=0.005), urea (p=0.050), and blood urea nitrogen (p=0.050) levels were higher in the IR+curcumin group. While total antioxidant status was found to be high in the IR+curcumin group (p=0.021), there was no difference in total oxidant status across the groups (p=0.069). Interleukin (IL)-1β and IL-6 were found to be higher in the IR group (p=0.014, p=0.022, respectively). Tumour necrosis factor-α was higher in the IR group than in other groups (p=0.020). Interferon-γ did not differ across groups (p=0.140). In the histopathological examination, the IR group had more damage to the glomerulus and tubular epithelial cells than the other groups (p<0.001). Conclusion: Curcumin, despite its anti-inflammatory and antioxidant characteristics, had no protective effect on renal functions in IR-induced kidney injury. It was found that it suppresses the inflammatory response and is effective in preserving renal tissue structure.

Protective effects of safranal on kidney tissue in a rat model of distant ischemiareperfusion injury with infrarenal aortic occlusion

“…The IR experimental model in the current study was established by collecting samples from the rats after 1 h of ischemia and 2 h of reperfusion, in accordance with previous studies [ 3 , 10 , 11 ]. In addition, safranaline was administered as a single dose of 1 mg/kg [ 10 , 30 ].…”

“…Xylazine HCl (Rompun; 23.32 mg/mL 50 mL Viel Bayer, İstanbul, Türkiye) was injected intramuscularly at a dose of 13 mg/kg as premedication, and the rats were anesthetised with ketamine HCl (Ketalar; 50 mg/mL, 10 mL Viel Parke-Davis, Eczacıbaşı, Türkiye) at a dose of 87 mg/kg injected intramuscularly [ 3 , 10 , 11 ]. In contrast to the other groups, rats in the IR-safranal group received 1 mg/kg of safranal (Sigma-Aldrich: W338907, Merck KGaA, Darmstadt, Germany) 30 min before laparotomy [ 10 , 11 ]. After anaesthesia, the rats were placed on the operating table.…”

“…At the end of the second hour of reperfusion, the rats were euthanized with an intraperitoneal injection of 150 mg/kg of thiopental sodium (Pental Na, İ.E. Ulagay, Türkiye) [ 3 , 11 ].…”

“…Lesions observed on the stained slides, including congestion, cellular vacuolization, interstitial oedema, luminal debris, and intratubular detachment, were scored between 0 and 4 points. On observation of no abnormality was scored as 0, mild lesions affecting 25% of the kidney specimens was scored as 1, lesions affecting 25%–50% was scored as 2, lesions affecting 50%–75% was scored as 3, and lesions affecting more than 75% was scored as 4 points [ 11 ]. The scores were summed, and the total histopathological damage score was calculated for each group.…”