Protective effects of chlorogenic acid on inflammatory responses induced by Staphylococcus aureus and milk protein synthesis in bovine mammary epithelial cells

Ji, Qiang; Zhang, Meng; Wang, Yanfeng; Chen, Yuhao; Wang, Liping; Lu, Xinyue; Bai, Linfeng; Wang, Manshulin; Bao, Lili; Huang, Hao; Wang, Zhigang

doi:10.1016/j.micpath.2022.105726

Cited by 6 publications

(4 citation statements)

References 54 publications

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“…Moreover, CGA reverses the inflammation-mediated decline in the transcription factor SF-1, that reduces expression of the milk protein genes LALBA, BLG, and CSN2. [119] Thus, further studies to explore CGA as an anti-inflammatory galactagogue in breastfeeding women may be important to explore.…”

Section: Effects Of Isolated Polyphenols On Lactationmentioning

confidence: 99%

Polyphenols and Lactation: Molecular Evidence to Support the Use of Botanical Galactagogues

Kelleher,

Burkinshaw,

Kuyooro

2024

Molecular Nutrition Food Res

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Botanicals and herbal supplements contain a diverse array of polyphenols that may affect mammary gland function and promote galactagogue activity. This scoping review is conducted to identify scientific literature elucidating how polyphenols affect mammary gland biology and cellular mechanisms critical for lactation. A literature search of PubMed and Medline reviews relevant studies in dairy animals, rodent models, and cultured mammary epithelial cells that are published from January 2010 until July 2023, to ascertain effects of polyphenols on mechanisms regulating milk production and composition. The PRISMA‐ScR (Preferred Reporting Items for Systematic Reviews and Meta‐Analyses for Scoping Review) strategy is applied and 80 studies on polyphenols and their implications on milk production and composition are included in this review. Limited information delineating effects of polyphenols on the molecular pathways that affect lactation are found, although available information suggests modulation of Stat5 signaling/differentiation, Stat3 signaling/remodeling, mTOR and insulin signaling/energy production, and nuclear factor kappa beta (NFκβ) signaling/oxidative stress and inflammation may play roles. A profound lack of mechanistic information underscores the critical need for further research to understand the impact of botanical supplements and polyphenols on milk production and composition in humans to establish maternal nutritional guidelines to support lactation and breastfeeding goals.

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Section: Effects Of Isolated Polyphenols On Lactationmentioning

confidence: 99%

Polyphenols and Lactation: Molecular Evidence to Support the Use of Botanical Galactagogues

Kelleher,

Burkinshaw,

Kuyooro

2024

Molecular Nutrition Food Res

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“…Upon activation, IκB undergoes phosphorylation and ubiquitination, facilitating the nuclear translocation of NF-κB to bind target genes, orchestrating the transcriptional modulation of the inflammatory cascade ( Liu and Yao, 2005 ). Chlorogenic acid’s capacity to mitigate inflammation via impeding this signaling conduit has been substantiated across diverse cellular and tissue frameworks ( Chen et al, 2021 ; Duan et al, 2022 ; Ji et al, 2022 ; Zhang and Xu, 2022 ). For instance, investigations have discerned that chlorogenic acid attenuates the inflammatory milieu in rheumatoid arthritis by suppressing NF-κB expression and its consequent DNA-binding activity, culminating in the inhibition of downstream TNF-α secretion ( Fu et al, 2019 ).…”

Section: Regulation Of Cellular Signaling Pathways By Chlorogenic Acidmentioning

confidence: 99%

Chlorogenic acid: a review on its mechanisms of anti-inflammation, disease treatment, and related delivery systems

Huang,

Xie,

et al. 2023

Front. Pharmacol.

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Chlorogenic acid is a bioactive compound ubiquitously present in the natural realm, lauded for its salient anti-inflammatory and antioxidant attributes. It executes its anti-inflammatory function by moderating the synthesis and secretion of inflammatory mediators, namely, TNF-α, IL-1β, IL-6, IL-8, NO, and PGE2. Concurrently, it modulates key signaling pathways and associated factors, including NF-κB, MAPK, Nrf2, and others, bestowing protection upon cells and tissues against afflictions such as cardio-cerebrovascular and diabetes mellitus. Nevertheless, the inherent low bioavailability of chlorogenic acid poses challenges in practical deployments. To surmount this limitation, sophisticated delivery systems, encompassing liposomes, micelles, and nanoparticles, have been devised, accentuating their stability, release mechanisms, and bioactivity. Given its innate anti-inflammatory prowess and safety profile, chlorogenic acid stands as a promising contender for advanced biomedical investigations and translational clinical endeavors.

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“…Mastitis causes a decrease in milk quality and quantity in lactating mammary glands in cows [ 51 ]. At the cellular level, the expression levels of factors related to milk production are decreased in cultured mouse and bovine MECs in association with inactivation of STAT5 [ 30 , 52 ]. In addition, the increase in CLDN4 expression and translocation of CLDN3 occurs concurrently with the weakness of the TJ barrier by injection of lipopolysaccharide, one of the endotoxins from Escherichia coli in mouse MECs [ 53 ].…”

Section: Overview Of Alveolar Luminal Mammary Epithelial Cell (Mec) F...mentioning

confidence: 99%

Culture Models to Investigate Mechanisms of Milk Production and Blood-Milk Barrier in Mammary Epithelial Cells: a Review and a Protocol

Kobayashi

2023

J Mammary Gland Biol Neoplasia

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Mammary epithelial cells (MECs) are the only cell type that produces milk during lactation. MECs also form less-permeable tight junctions (TJs) to prevent the leakage of milk and blood components through the paracellular pathway (blood-milk barrier). Multiple factors that include hormones, cytokines, nutrition, and temperature regulate milk production and TJ formation in MECs. Multiple intracellular signaling pathways that positively and negatively regulate milk production and TJ formation have been reported. However, their regulatory mechanisms have not been fully elucidated. In addition, unidentified components that regulate milk production in MECs likely exist in foods, for example plants. Culture models of functional MECs that recapitulate milk production and TJs are useful tools for their study. Such models enable the elimination of indirect effects via cells other than MECs and allows for more detailed experimental conditions. However, culture models of MECs with inappropriate functionality may result in unphysiological reactions that never occur in lactating mammary glands in vivo. Here, I briefly review the physiological functions of alveolar MECs during lactation in vivo and culture models of MECs that feature milk production and less-permeable TJs, together with a protocol for establishment of MEC culture with functional TJ barrier and milk production capability using cell culture inserts.

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Protective effects of chlorogenic acid on inflammatory responses induced by Staphylococcus aureus and milk protein synthesis in bovine mammary epithelial cells

Cited by 6 publications

References 54 publications

Polyphenols and Lactation: Molecular Evidence to Support the Use of Botanical Galactagogues

Polyphenols and Lactation: Molecular Evidence to Support the Use of Botanical Galactagogues

Chlorogenic acid: a review on its mechanisms of anti-inflammation, disease treatment, and related delivery systems

Culture Models to Investigate Mechanisms of Milk Production and Blood-Milk Barrier in Mammary Epithelial Cells: a Review and a Protocol

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