Protective Effects of Carbon Dots Derived from Armeniacae Semen Amarum Carbonisata Against Acute Lung Injury Induced by Lipopolysaccharides in Rats

Abstract

Introduction:The charcoal processed product of Armeniacae Semen Amarum (ASA), ASA Carbonisata (ASAC), has long been used for its anti-inflammatory effects. However, the material basis and mechanism of action of ASAC remain unclear. Aim: To explore the anti-inflammatory effects of Armeniacae Semen Amarum Carbonisataderived carbon dots (ASAC-CDs). Methods: The physicochemical properties of ASAC-CDs including morphology, optical properties, functional groups were characterized by a series of methods, mainly including electron microscopy, optical technology and X-ray photoelectron spectroscopy. The antiinflammatory effect of ASAC-CDs was evaluated and confirmed using acute lung injury (ALI) induced by lipopolysaccharides (LPS) in rats. Results:The ASAC-CDs ranged from 1.5 to 5.5 nm in diameter, with a quantum yield of 3.17%. ASAC-CDs alleviated LPS-induced inflammation, as demonstrated by reducing the levels of IL-6, IL-1β and TNF-α and increasing the contents of IL-10 in rat serum. More interestingly, ASAC-CDs reduce the content of MDA and MPO and increase the activity of SOD and the content of GSH, indicating the antioxidant activity of ASAC-CDs. Conclusion:These results demonstrate the remarkable anti-inflammatory effects of ASAC-CDs against ALI induced by LPS, which provide an important basis for the application of ASAC-CDs in clinical anti-pneumonia, and lay an experimental foundation for the research and development of novel nano-drugs.

Aspirin eugenol ester alleviates lipopolysaccharide-induced acute lung injury in rats while stabilizing serum metabolites levels

“…According to the preliminary results of in vitro experiments, in vivo experiments were also conducted. Experimental animals were selected as Wistar rats, and the modeling drug was chosen as LPS with the concentration of 5 mg•kg -1 (46,47).…”

“…Neutrophil aggregation is a key character of inflammation in the lung. MPO is a marker of neutrophil function and activation and its activity level can implicitly reflect the extent of neutrophil aggregation at the site of inflammation (46). Therefore, neutrophils were not tested in this study, but the changes in neutrophils could be inferred from the changes in MPO.…”

Carbon Dots Derived from Traditional Chinese Medicines with Bioactivities: A Rising Star in Clinical Treatment

“…TCMs-CDs have attracted extensive research interest in the biomedical treatment of inflammation by activating the expression of anti-inflammatory factors. At present, CDs have been made from mulberry silkworm cocoon (MSC), Lonicerae japonicae Flos carbonisata (LJFC), Armeniacae Semen Amarum carbonisata (ASAC), AFIC, Puerariae lobatae radix (PLR), radix Sophorae Flavescentis carbonisata (RSFC), and these TCMs-CDs have significant anti-inflammatory activities.…”

“…Moreover, the presence of CDs encourages the mRNA expression of associated genes, producing additional antioxidant proteins that protect zebrafish from oxidative damage . From the study of Zhao et al, ASAC-CDs can increase SOD activity and GSH content in rat lung tissue to some extent, suggesting that ASAC-CDs have antioxidant action. Using 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and H 2 O 2 scavenging activities, ferroelectric reduction power, and Ferric ion reducing antioxidant power (FRAP) test, Neeraj Tejwan et al evaluated the radical scavenging capability of the CDs from red Korean ginseng in a cell-free system.…”

“…Inflammation is a dynamic process that includes injury, anti-injury, and repair. 22 Lonicerae japonicae Flos carbonisata (LJFC), 104 Armeniacae Semen Amarum carbonisata (ASAC), 105 AFIC, 102 Puerariae lobatae radix (PLR), 103 radix Sophorae Flavescentis carbonisata (RSFC), 106 and these TCMs-CDs have significant anti-inflammatory activities. Wu et al 104 explored the effects of LJFC-CDs on lipopolysaccharide (LPS)-induced fever and hypothermia rat models.…”