Protective effects of caffeic acid phenethyl ester on the dose‐dependent acute nephrotoxicity with paraquat in a rat model

Abstract

Paraquat (PQ), which is used extensively as a potent herbicide throughout the world, is highly toxic in humans. We aimed to determine PQ-induced biochemical and histologic changes in the kidneys, and to evaluate the ability of the protective effects of caffeic acid phenethyl ester (CAPE) against PQ-induced injury in rats. Forty-eight rats were divided into eight groups of six: Group 1: Control; Group 2: 10 μmol/kg CAPE; Group 3: 15 mg/kg PQ; Group 4: 30 mg/kg PQ; Group 5: 45 mg/kg PQ; Group 6: 15 mg/kg PQ+CAPE; Group 7: 30 mg/kg PQ+CAPE; Group 8: 45 mg/kg PQ+CAPE. PQ and CAPE were injected intraperitoneally. The levels of the total oxidant status (TOS) and total antioxidant status (TAS) were determined in the supernatants of the excised left kidney. Right kidney tissue of each rat was removed to obtain a histologic score. When PQ-administrated (15, 30, 45) groups compared with other groups, TOS values were found to be significantly higher (p < 0.01). PQ (15, 30, 45) groups had significantly diminished values of TAS than the other groups (p < 0.001). Of histologic score evaluation, only the PQ45 group had a significantly higher value than the sham, and CAPE groups (p < 0.05). Moreover, in CAPE+PQ45 group, the level of histologic score was decreased compared to PQ45 group (p < 0.001). In conclusion, the evaluation of the data suggests that CAPE can be used to prevent the acute effects of PQ nephrotoxicity.

Alpha-Amanitin Poisoning, Nephrotoxicity and Oxidative Stress: An Experimental Mouse Model

“…There have also been different clinical studies on TAS and TOS measurements as an indicator of oxidative/antioxidative balance ( 38 - 40 ). In experimental studies investigating the relationship between oxidative stress following toxic exposure and nephrotoxicity, we observe that while the TOS level increased significantly in the presence of oxidative stress, the TAS level decreased significantly ( 41 , 42 ). However, we could not find any studies in the literature investigating TAS and TOS activity in renal cells following in vivo α-AMA exposure.…”

Ferulic acid grafted into β‐cyclodextrin nanosponges ameliorates Paraquat‐induced human MRC‐5 fibroblast injury

“…Death from Paraquat poisoning is the main clinical problem in the world. 45,46 Local and systemic effects are produced by PQ toxicity, which damages multiple tissues and organs in humans 47 and animals. 48,49 The most considerable damage happens to the lung tissue 50,51 owing to the accumulation and uptake of Paraquat by lung tissue.…”

Caffeic Acid Phenethyl Ester as a Protective Agent against Nephrotoxicity and/or Oxidative Kidney Damage: A Detailed Systematic Review

“…Therefore, several studies have been conducted to find an available agent to prevent paraquat toxicity. Rifaioglu et al investigated paraquat-induced biochemical and histological changes in kidney, one of main targets of paraquat, and the possible protective effects of CAPE in rats [ 74 ]. By measuring two important ROS-related parameters, total antioxidant capacity and total oxidant status, as well as histologic scores of affected kidneys, they found that CAPE can be used to prevent the acute effects of paraquat nephrotoxicity.…”