Protective effects of buckwheat honey on DNA damage induced by hydroxyl radicals

Zhou, Juan; Li, Peng; Cheng, Ni; Gao, Hui; Wang, Bini; Wei, Yahui; Cao, Wei

doi:10.1016/j.fct.2012.05.046

Cited by 58 publications

(45 citation statements)

References 46 publications

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“…The thymine radical then undergoes a series of reactions, including reaction with O 2 to give a thymine peroxyradical (Dizdaroglu & Jaruga, 2012). Thus, if OHÁ is generated adjacent to DNA, it damages the bases (and deoxyribose sugar) and induces strand breaks (Zhou et al, 2012). The DNA damage was analyzed by single cell gel electrophoresis (comet assay) and DNA fragmentation assay.…”

Section: Discussionmentioning

confidence: 99%

Phytochemical investigation and radical scavenging activities ofMelia azedarachand its DNA protective effect in cultured lymphocytes

Srinivasan¹,

Balakrishnan²,

Nair³

2013

Pharmaceutical Biology

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Context: Melia azedarach Linn (Meliaceae) is an Ayurvedic medicinal plant which is native to India. It is traditionally used for the treatment of leprosy, inflammation, scrofula, anthelmintic, antilithic, diuretic, deobstruent and cardiac disorders. Objective: To evaluate the phytochemical constituents and antioxidant activities of the ethanol leaf extract of Melia azedarach (MA) and its protective effect against H 2 O 2 -induced cellular damage in cultured lymphocytes. Materials and methods:The dose-dependent study of MA (20, 40, 60, 80, 100 mg/ml) was used to study in vitro radical scavenging assays. The effective dose of MA (60 mg/ml) was further used to study the H 2 O 2 -induced DNA damage (comet assay and DNA fragmentation assay) in cultured lymphocytes. Results: The ethanol extract of MA (20, 40, 60, 80, 100 mg/ml) exhibited a significant dosedependent inhibition of in vitro radical scavenging assays and their corresponding IC 50 values as follows: hydroxyl radical (26.50 AE 0.26 mg/ml), superoxide anion (30.00 AE 0.32 mg/ml), nitric oxide radical (48.00 AE 0.48 mg/ml), DPPH radical (30.55 AE 0.32 mg/ml) and reducing power (22.00 AE 0.22 mg/ml). The increase in the severity of DNA damage and TBARS was increased significantly (p50.05) at 500 mM H 2 O 2 -treated cultured lymphocytes and RBC cellular membranes. The phytochemical screening studies identified 13 chemical constituents present in the leaf extract of MA. Discussion and conclusion: The results of this study demonstrate that MA offers protection against H 2 O 2 -induced cellular damage and it can be developed as an effective antioxidant during oxidative stress.

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Section: Discussionmentioning

confidence: 99%

Phytochemical investigation and radical scavenging activities ofMelia azedarachand its DNA protective effect in cultured lymphocytes

Srinivasan¹,

Balakrishnan²,

Nair³

2013

Pharmaceutical Biology

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“…9, No. 13;2017 150 In the non-specific system, plasmid DNA which present in supercoiled form decrease by 68.6% when treated with honey (Zhou et al, 2012). Propolis also has a potential to increase the expression of DNA repair enzyme such as hOGG-1 and NEIL-1 (Turan et al, 2015).…”

Section: In Vitro Studiesmentioning

confidence: 99%

“…13; 2017 Table 1 summarizes the effect of honey and its derivatives on genome health in in vitro studies. Published articles claimed that honey, propolis and bee pollen can reduce the DNA damage induced by genotoxic agent such as benzo (α) pyrene (Russo et al, 2006), peroxynitrate (Luo et al, 2007), hydrogen peroxide (Afroz et al, 2016;Cheng et al, 2013;Habib et al, 2014;Sherin et al, 2015;Zhou et al, 2012), chlorpyrifos (pesticide) (Alleva et al, 2016) and tertiary-butyl-hydroperoxide (Turan et al, 2015). Generally, antioxidant can scavenge free radicals and reduce the potential of oxidative damage in the cell.…”

Section: Alves De Lima Et Al (2005)mentioning

confidence: 99%

“…Generally, antioxidant can scavenge free radicals and reduce the potential of oxidative damage in the cell. There was a study conducted by Zhou et al (2012) stated that honey has a capability to protect DNA in the non-specific system from being damaged by ROS.…”

Section: Alves De Lima Et Al (2005)mentioning

confidence: 99%

“…Most of the published articles showed that the honey and its derivatives can reduce the DNA damage by scavenging of the reactive oxygen species (ROS) when cell or mice were induced with genotoxic agents (Cheng et al, 2015(Cheng et al, , 2017Kumari et al, 2017;Zhou et al, 2012). Reducing or inhibiting the free radical can decrease the potential of having genotoxic related diseases such as cancer, inflammation disorder and cardiovascular disease (Wen et al, 2012).…”

Section: In Vivo Studiesmentioning

confidence: 99%

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Current Knowledge on Honey and Its Derivatives with Genomic Stability: A Mini Review

Yaacob¹,

Stanis²,

Rajab³

et al. 2017

JAS

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Genome health is an important factor that plays a role in various degenerative diseases. Instability of genome is the prevalence of mutation within the genome such as changed in nucleic acid and chromosomal arrangement and also the presence of abnormal number of chromosome in cell. Therefore, several method were used to overcome this problem and one of them is by using natural product such as honey, propolis, bee pollen and royal jelly that is high in antioxidant. Those are products that derive from honey bee and had been used as food supplement to increase the quality of life. Therefore, this systematic review provides the updates on the potential of honey bee products to decrease DNA damage both in in vivo and in vitro approaches. Search term of "honey", "propolis", "bee pollen", royal jelly", "DNA damage", "genome integrity", and "telomere" were used for searching purpose in three databases (Scopus, Pubmed and Medline) and also Google Scholar. All the published articles were assessed using PRISMA guidelines and finally after the eligibility process, only 34 published articles were selected for this review. Based on the reports, the product from the honey bee decrease the genome related diseases by reducing the accumulation of free radical, increase the DNA repair protein expression and decrease the telomerase activity in the cell. This provides a large gap in the research field focusing on the effect of those derivatives from bees on genomic stability.

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Reformulation of spray‐dried apple concentrate and honey for the enhancement of drying process performance and the physicochemical properties of powders

et al. 2020

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BACKGROUND Apple concentrate and honey were spray‐dried with traditional carriers (maltodextrin, Nutriose®, Kleptose®) and with skimmed milk powder as a carrier substitute offering added value for the final product. RESULTS Skimmed milk powder, although having the lowest glass transition temperature, was the best carrier in terms of the highest powder recovery (higher than 80% was achieved) and with powder physical properties comparable to those obtained with other carriers. Thus, skimmed milk powder was used to produce honey powder of reduced carrier content (to 25% solids). The physical properties of the powders obtained and lower powder recovery indicated more problematic drying of AC. Powders produced with skimmed milk were characterized by enhanced nutritional value compared with variants with traditional carriers. CONCLUSION Thus, skimmed milk is proposed as a natural carrier for spray‐drying, offering high processing yield, the possibility to reduce carrier content in honey powder, and to create products of nutritional added value. © 2020 Society of Chemical Industry

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Protective effects of buckwheat honey on DNA damage induced by hydroxyl radicals

Cited by 58 publications

References 46 publications

Phytochemical investigation and radical scavenging activities ofMelia azedarachand its DNA protective effect in cultured lymphocytes

Phytochemical investigation and radical scavenging activities ofMelia azedarachand its DNA protective effect in cultured lymphocytes

Current Knowledge on Honey and Its Derivatives with Genomic Stability: A Mini Review

Reformulation of spray‐dried apple concentrate and honey for the enhancement of drying process performance and the physicochemical properties of powders

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