Protective effects of boron on cyclophosphamide induced lipid peroxidation and genotoxicity in rats

İnce, Sinan; Küçükkurt, İ̇smail; Demirel, Hasan Hüseyin; Acaröz, Damla Arslan; Akbel, Erten; Çiğerci, İbrahim Hakkı

doi:10.1016/j.chemosphere.2014.01.038

Cited by 104 publications

(47 citation statements)

References 52 publications

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“…In addition to in vitro studies, research evaluating arsenic‐induced chromosomal aberration, sister chromatid exchanges, and hypermethylation or hypomethylation of DNA in mice, rats, fish, and humans has been conducted . In the present work, arsenic treatment caused increased levels of DNA damage in rat mononuclear leukocytes, whereas boron (particularly at doses of 10 and 20 mg/kg) showed antigenotoxic effects and protected cells from DNA damage with its cell protective effect …”

Section: Discussionsupporting

confidence: 51%

“…In the current study, boron (particularly at doses of 10 and 20 mg/kg) suppressed the expression of proinflammatory cytokine genes (IFN‐γ, IL‐1β, TNF‐α, and NFκB). This may be explained by the antioxidant effects of boron, which inhibit the generation of ROS.…”

Section: Discussionmentioning

confidence: 99%

“…The first group, served as control, was treated orally (vehicle treated) with physiological saline, and the second group was treated with arsenic at the dose of 100 mg/L given in drinking water. The third, fourth, and fifth groups were treated with arsenic (100 mg/L in a drinking water) and boron at 5, 10, and 20 mg/kg/d by gastric gavage, respectively for 30 days.…”

Section: Methodsmentioning

confidence: 99%

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Boron ameliorates arsenic‐induced DNA damage, proinflammatory cytokine gene expressions, oxidant/antioxidant status, and biochemical parameters in rats

İnce

Küçükkurt

Acaröz

et al. 2018

J Biochem & Molecular Tox

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Arsenic, an element found in nature, causes hazardous effects on living organisms. Meanwhile, natural compounds exhibit protective effects against hazardous substances. This study evaluated the effects of boron against arsenic-induced genotoxicity and altered biochemical parameters in rats. Thirty-five male Wistar albino rats were equally divided into five groups, and the experimental period lasted 30 days. One group was used as the control, and another group was treated with 100 mg/L arsenic in drinking water. The other groups were orally treated with 5, 10, and 20 mg/kg boron plus arsenic (100 mg/L via drinking water). Arsenic caused changes in biochemical parameters, total oxidant/antioxidant status, and DNA damage in mononuclear leukocytes. Moreover, it increased IFN-γ, IL-1β, TNF-α, and NFκB mRNA expression levels in rat tissue. However, boron treatment improved arsenic-induced alterations in biochemical parameters and increases in DNA damage and proinflammatory cytokine gene expressions.

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Section: Discussionsupporting

confidence: 51%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Boron ameliorates arsenic‐induced DNA damage, proinflammatory cytokine gene expressions, oxidant/antioxidant status, and biochemical parameters in rats

İnce

Küçükkurt

Acaröz

et al. 2018

J Biochem & Molecular Tox

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“…A recent study concluded that boron compounds increase antioxidant enzyme activities and prevent lipid peroxidation (LPO), thus showing an antioxidant effect . At the same time, boron demonstrates hepatoprotective and DNA damage preventing effects . The aim of the present study was to examine the effect of boron antioxidant efficiency of which is known against exposure to arsenic, which is prevalent in nature and especially in waters.…”

Section: Introductionmentioning

confidence: 96%

The Effects of Boron on Arsenic-Induced Lipid Peroxidation and Antioxidant Status in Male and Female Rats

Küçükkurt

İnce

Demirel

et al. 2015

J Biochem Mol Toxicol

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The aim of the present study was to investigate the possible protective effects of boron, an antioxidant agent, against arsenic-induced oxidative stress in male and female rats. In total, 42 Wistar albino male and female rats were divided into three equal groups: The animals in the control group were given normal drinking water, the second group was given drinking water with 100 mg/L arsenic, and the third group was orally administered drinking water with 100 mg/kg boron together with arsenic. At the end of the 28-day experiment, arsenic increased lipid peroxidation and damage in the tissues of rats. However, boron treatment reversed this arsenic-induced lipid peroxidation and activities of antioxidant enzymes in rats. Moreover, boron exhibited a protective action against arsenic-induced histopathological changes in the tissues of rats. In conclusion, boron was found to be effective in protecting rats against arsenic-induced lipid peroxidation by enhancing antioxidant defense mechanisms.

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“…4 CP is used in high doses for the chemotherapy of various forms of cancer, in low doses in the treatment of autoimmune diseases, and also as an immunosuppressant after organ transplants. 5 CP's chemically reactive metabolic products induce cytotoxicity by alkylating DNA and proteins. 3 CP is known as human carcinogen and has an increased incidence of chromosome aberrations in lymphocytes from patients with malignant and non-malignant diseases.…”

Section: Introductionmentioning

confidence: 99%

Assessment of <i>In Vitro</i> Antigenotoxic Effect of <i>Nigella Sativa</i> Oil

Zor¹,

Kurtoğlu²

2020

tjps

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ÖZAmaç: Siklofosfamid, antineoplastik ve immünosüpresif ajan olarak yaygın olarak kullanılan alkilleyici bir ajandır. Siklofosfamid'in genotoksisitesi çeşitli in vivo ve in vitro sistemde çalışılmış ve rutin olarak genotoksisite testlerinde pozitif kontrol olarak kullanılmaktadır. Geleneksel tıp da Nigella sativa L. (N. sativa), Ranunculaceae familyasından olup günümüzde başta Doğu Akdeniz ülkeleri olmak üzere birçok ülkede yaygın olarak yetişen ve hem baharat hem de halk ilacı olarak Dioscorides zamanından beri kullanılan bir bitkidir. Bu çalışmada Siklofosfamid'in genotoksik etkilerine karşı çörek otu yağının değişik konsantrasyonlardaki koruyucu etkilerininin mikronükleus testi ile gösterilmesi amaçlanmıştır. Gereç ve Yöntemler: Bu amaçla, sağlıklı hücreler 1, 5, 10 µg/mL konsantrasyonlarında N. sativa yağı ile ve pozitif kontrol olarak Siklofosfamid ile 68 saat boyunca in vitro muamele edildi. Daha sonra ise mikronükleuslar sayıldı. Bulgular: N. sativa yağının 1, 5, 10 µg/mL konsantrasyonlarındaki uygulaması negatif kontrolle karşılaştırıldığında mikronükleus frekansında anlamlı bir artış gözlenmedi. N. sativa yağı ve Siklofosfamid'in birlikte uygulandığı gruplarda her üç konsantrasyonda da (1, 5, 10 µg/mL) Siklofosfamid grubuna göre mikronükleus sayısında azalma olduğu görüldü. Sonuç: N. sativa yağının genotoksisite ajanlarına karşı in vitro koruyucu etkilerinin olabileceği gösterilmiştir. N. sativa yağının genotoksisiteyi giderici etkilerinin mekanizmasının anlaşılması için daha fazla çalışmaya ihtiyaç vardır. ABSTRACTObjectives: Cyclophosphamide (CP) is an alkylating agent widely used as an antineoplastic and immunosuppressive agent. The genotoxicity of CP has been studied in a variety of in vivo and in vitro systems and is routinely used as a positive control in genotoxicity tests. Traditional medicine Nigella sativa L., (N. sativa), Ranunculaceae family, especially in the Eastern Mediterranean countries, especially in many countries, and is widely used in many countries as a spice and folk medicine since the time of Dioscorides used as a plant. In this study, it was aimed to show the protective effects of N. sativa oil at different concentrations against the genotoxic effects of CP by micronucleus test. Materials and Methods: For this purpose, healthy cells were treated in vitro with N. sativa oil at concentrations of 1, 5, 10 µg/mL and CP as positive control for 68 hours. The micronuclei were then counted. Results: No significant increase in micronucleus frequency was observed when the application of N. sativa oil at concentrations of 1, 5, 10 µg/mL compared with the negative control. There was a decrease in the number of micronucleus in all three concentrations (1, 5, 10 µg/mL) compared to the CP group in the groups treated with N. sativa oil and CP. Conclusion: It has been shown that N. sativa oil may have protective effects against genotoxicity agents in vitro. But more work is needed to understand the mechanism of the genotoxicity effects of N. sativa oil.

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Protective effects of boron on cyclophosphamide induced lipid peroxidation and genotoxicity in rats

Cited by 104 publications

References 52 publications

Boron ameliorates arsenic‐induced DNA damage, proinflammatory cytokine gene expressions, oxidant/antioxidant status, and biochemical parameters in rats

Boron ameliorates arsenic‐induced DNA damage, proinflammatory cytokine gene expressions, oxidant/antioxidant status, and biochemical parameters in rats

The Effects of Boron on Arsenic-Induced Lipid Peroxidation and Antioxidant Status in Male and Female Rats

Assessment of <i>In Vitro</i> Antigenotoxic Effect of <i>Nigella Sativa</i> Oil

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