Protective Effects of Beetroot Extract against Phenyl Hydrazine Induced Anemia in Rats

Abstract

Background: Anemia can be regarded as one of the oldest blood malformation known over a century. Megaloblastic anemia arises due to curtailed formation of erythrocytes, which leads to formation of a large number of underdeveloped erythrocytes. Proper nutritional supplementation may be useful in such condition. Beta vulgaris or beetroot is one of the important vegetables consumed worldwide. The aim of the present work was to evaluate anti-anemic potential of beetroot. Methods: Beetroot was extracted with ethanol. Phytochemical and phytoanalytical studies were performed on extract. Anemia was induced by phenyl-hydrazine. Animals were treated with extract throughout the study for 24 days. The red blood cell (RBC) number and hemoglobin concentration were determined every 3 days for 24 days. Results: Extract was found to be rich in folic acid, ascorbic acid, and iron. Following the induction of anemia, the number of erythrocytes and the hemoglobin concentration decreased by 62.51% and 69.64%, respectively. Administration of standard hematinic preparation and extract (200 mg/kg) resulted in signifi cant increase (P < 0.001; P < 0.01) in the number of RBCs as well as hemoglobin concentration when compared to the untreated phenyl hydrazine-induced anemic rats. Conclusion: Extract effectively raised the level of hemoglobin and erythrocyte count at dose 200 mg/kg. Vitamin and minerals found in beetroot are most likely active ingredients responsible for its hematinic effects. Still, methodical studies are obligatory to derive its effects on humans.

The anti-anemic effect of dried beet green in phenylhydrazine treated rats

“…B 12 and folic acid in the beetroot leaf which could be reversed the effects of phenylhydrazine drug. These results are agreements with vitamin and minerals found in beetroot are most likely active ingredients responsible for its hematinic effects [45]. On the other hand, the highest significant increases of RBC count, HGB concentration and HCT percentage showed in group II compared with control group at D21 and D42 days.…”

Laboratory evidence for the hematopoietic potential of Beta vulgaris leaf and stalk extract in a phenylhydrazine model of anemia

“…For instance, Jaiswal et al (25) showed that treatment of anemic rats with extracts of the plant root prepared by hot percolation in a Soxhlet apparatus significantly reversed the decrements of RBCs count and HGB concentration. More recently, Al-aboud (26) found that taking eight grams of beetroot for 20 days by female volunteers resulted in mild increase in hemoglobin, ferritin, and serum iron levels.…”

Effects of Sweet Potato Leaves, Roselle Calyces and Beetroot on Body Weight, Selected Hematological and Biochemical Parameters in Broiler Chicken

“…Normal red cell production requires diet containing protein, iron, copper, vitamin B 2 , B 6 , B 12 and folic acid (Nyaulingo, 2013). The study done by Jaiswal et al, (2014) reported that administration of 200 mg/kg beetroot extract to phenyl hydrazine-induced anemic rats significantly increased (p < 0.01) the number of RBCs as well as hemoglobin concentration when compared to the untreated group. Beetroot was found to be rich in folic acid, ascorbic acid, iron, vitamin and minerals which are most likely active ingredients responsible for its haematinic effects.…”