Protective effects of bee bread on testicular oxidative stress, NF-κB-mediated inflammation, apoptosis and lactate transport decline in obese male rats

Suleiman, Joseph Bagi; Nna, Victor Udo; Zakaria, Zaida; Othman, Zaidatul Akmal; Eleazu, Chinedum; Bakar, Ainul Bahiyah Abu; Ahmad, Azlina; Usman, Umar; Rahman, Wan Faiziah Wan Abdul; Mohamed, Mahaneem

doi:10.1016/j.biopha.2020.110781

Cited by 21 publications

(27 citation statements)

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“…MAFLD is a metabolic disorder associated with obesity, hyperlipidemia, hyperglycemia, insulin resistance, oxidative stress and inflammation [ 61 ]. There are now growing evidences that have claimed the health benefits of bee bread in reducing the risk of metabolic syndrome [ 38 , 41 , 42 , 43 , 44 , 45 ]. Consequently, this study was performed to investigate, for the first time, the possible antioxidative, anti-inflammatory, anti-steatotic and anti-fibrotic effects of bee bread in the HFD-induced MAFLD rat model.…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies have demonstrated that bee bread possesses hepatoprotective properties as shown by decreases in the severity of liver damage in alcoholic fatty liver disease patients [ 38 ], and in aluminum [ 39 ] and carbon tetrachloride (CCl 4 )-induced liver injury [ 40 ] in rats. Bee bread has also shown its medicinal effects in other varieties of ailments including cardiovascular diseases [ 41 ], male fertility [ 42 ], renal dysfunction [ 43 ] and cancer [ 44 ] in in vitro and in vivo studies. Nevertheless, there is only one set of data demonstrating the effects of bee bread on the high-fat diet (HFD)-induced fatty liver disease rat model, which reported that the consumption of bee bread (80, 400 and 800 mg/kg/day) downregulated the expressions of hepatic lipogenic protein and genes such as fatty acid synthase (FAS) and acetyl-CoA carboxylase (ACC) levels, hence inhibiting hepatic lipid synthesis [ 45 ].…”

Section: Introductionmentioning

confidence: 99%

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Hepatoprotective Effect of Bee Bread in Metabolic Dysfunction-Associated Fatty Liver Disease (MAFLD) Rats: Impact on Oxidative Stress and Inflammation

et al. 2021

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Metabolic dysfunction-associated fatty liver disease (MAFLD) is a pathological accumulation of hepatic lipid closely linked with many metabolic disorders, oxidative stress and inflammation. We aimed to evaluate the hepatoprotective effect of bee bread on oxidative stress and inflammatory parameters in MAFLD rats. Twenty-eight male Sprague-Dawley rats were assigned into four groups (n = 7/group): normal control (NC), high-fat diet (HFD), bee bread (HFD + Bb, HFD + 0.5 g/kg/day bee bread) and orlistat (HFD + Or, HFD + 10 mg/kg/day orlistat) groups. After 12 weeks, the HFD group demonstrated significantly higher body weight gain, serum levels of lipids (TG, TC, LDL), liver enzymes (AST, ALT, ALP) and adiponectin, liver lipids (TG, TC) and insulin resistance (HOMA-IR). Furthermore, the HFD group showed significantly decreased antioxidant enzyme activities (GPx, GST, GR, SOD, CAT) and GSH level, and increased liver oxidative stress (TBARS, NO), translocation of Nrf2 to the nucleus, Keap1 expression and inflammation (TNF-α, NF-κβ, MCP-1) together with histopathological alterations (steatosis, hepatocyte hypertrophy, inflammatory cell infiltration, collagen deposition), which indicated the presence of non-alcoholic steatohepatitis (NASH) and fibrosis. Bee bread significantly attenuated all these changes exerted by HFD feeding. In conclusion, our results suggest that bee bread might have antioxidant, anti-inflammatory, anti-steatotic and anti-fibrotic effects that are beneficial in protecting liver progression towards NASH and fibrosis.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Hepatoprotective Effect of Bee Bread in Metabolic Dysfunction-Associated Fatty Liver Disease (MAFLD) Rats: Impact on Oxidative Stress and Inflammation

et al. 2021

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“…Several studies have demonstrated that treatment with orlistat ameliorated metabolic variables [31][32][33] and oxidative stress [34,35] in obese rats. Our previous research reported an up-regulation of Nrf2 gene expression in the testis of HFD rats after orlistat intake for 12 weeks [36]. Nevertheless, no finding has been reported on the activation of Nrf2 in the liver after orlistat administration.…”

Section: Introductionmentioning

confidence: 89%

Protective and Therapeutic Effects of Orlistat on Metabolic Syndrome and Oxidative Stress in High-Fat Diet-Induced Metabolic Dysfunction-Associated Fatty Liver Disease (MAFLD) in Rats: Role on Nrf2 Activation

Zakaria

Othman

Suleiman

et al. 2021

Veterinary Sciences

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Metabolic dysfunction-associated fatty liver disease (MAFLD) is an excessive buildup of liver lipids closely associated with various kinds of undesirable metabolic effects and oxidative stress. We aimed to investigate the protective and therapeutic effects of orlistat on metabolic syndrome and oxidative stress parameters in high-fat diet (HFD) induced-MAFLD rats. Twenty-four male Sprague-Dawley rats were randomly divided into four groups (n = 6/group), i.e., Normal control (N), HFD, HFD + orlistat (HFD + O) (10 mg/kg/day administered concomitantly for 12 weeks as a protective model), and obese+orlistat (OB + O) (10 mg/kg/day administered 6 weeks after induction of obesity as a therapeutic model) groups. After 12 weeks, the HFD group had significantly increased Lee obesity index, serum alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, total cholesterol, triglyceride, low-density lipoprotein levels, liver total cholesterol and triglyceride levels, insulin resistance and non-alcoholic steatohepatitis (NASH) together with decreased serum high-density lipoprotein level. Additionally, the HFD group also showed increased Nrf2 translocation to the nucleus with high Keap1 expression and increased liver oxidative stress parameters. Orlistat significantly improved all these alterations in HFD rats. We demonstrated that orlistat might have protective and therapeutic effects against HFD-induced MAFLD rats by its activation on Nrf2 signaling pathway, which subsequently improved metabolic syndrome and oxidative stress parameters.

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“…In our previous study (Eleazu et al., 2020), we reported the modulatory properties of Malaysian BB on serum renal function parameters, oxidative stress, inflammatory, and B‐cell associated protein X (Bax) expression levels in the kidneys of high‐fat diet (HFD)‐induced obese rats. Further studies by our group also showed the protective effects of bee bread on testicular oxidative stress, NF‐κB‐mediated inflammation, apoptosis, and lactate transport decline in obese male rats (Suleiman et al., 2020).…”

Section: Introductionmentioning

confidence: 79%

Effect of bee bread on some biochemical parameters and skeletal muscle histology of high‐fat diet‐induced obese Sprague‐Dawley rats

et al. 2021

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Obesity is a disorder of body weight regulatory systems that is characterized by an accumulation of excess body fat due to hyperplasia and hypertrophy of adipocytes (Eleazu et al., 2019; Ferrier, 2014). Several factors have been implicated in the etiology of obesity including genetic and environmental factors, stress, disequilibrium in energy intake and expenditure, increased energy from gut/intestinal

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Protective effects of bee bread on testicular oxidative stress, NF-κB-mediated inflammation, apoptosis and lactate transport decline in obese male rats

Cited by 21 publications

References 56 publications

Hepatoprotective Effect of Bee Bread in Metabolic Dysfunction-Associated Fatty Liver Disease (MAFLD) Rats: Impact on Oxidative Stress and Inflammation

Hepatoprotective Effect of Bee Bread in Metabolic Dysfunction-Associated Fatty Liver Disease (MAFLD) Rats: Impact on Oxidative Stress and Inflammation

Protective and Therapeutic Effects of Orlistat on Metabolic Syndrome and Oxidative Stress in High-Fat Diet-Induced Metabolic Dysfunction-Associated Fatty Liver Disease (MAFLD) in Rats: Role on Nrf2 Activation

Effect of bee bread on some biochemical parameters and skeletal muscle histology of high‐fat diet‐induced obese Sprague‐Dawley rats

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