Protective Effects of B.W. 501 C 67 and B.W. 204 C 67 Against Lethal Effects of Exudates From Burnt Rat Skin

Abstract

Rocha e Silva and Rosenthal (1) MATERIALS AND METHODSThe method of Rocha e Silva and Rosenthal (1) was utilized to obtain the exudates. Albino rats weighing between 120 and 150 g were anaesthetized with nembutal sodium (30 mg/kg intraperitoneally) and then 30 ml of air was injected beneath the dorsal skin to raise a pouch of 7 x 4 x 3 cm. This done of the pocket was submerged in water at 95°C for 15 to 20 sec. After scalding, the animal was suspended by its paws and 5 ml of Tyrode's solution was injected into the pouch. The animal was then shaken for 2 min after which the fluid was withdrawn by syringe and needle. The washing was repeated at regular intervals after burning and a maximum of 5 washings were done to each animal. The fluids thus obtained were mixed and injected intraperitoneally into albino rats taken in groups of ten and the percentage mortality at three different dose levels were determined during the next 24 hr. Results were taken as control readings.B.W. 501 C 67 and B.W. 204 C 67 were injected intraperitoneally in different doses one hour before administration of the exudate in a dose of 5 ml/l00 g of body weight to each rat and at least three dose levels between 0 and l00 mortality were determined for each drug.