Protective effects of azarole polyphenolic extracts against oxidative damage using in vitro biomolecular and cellular models

Belkhir, Manel; Dhaouadi, Karima; Rosa, Antonella; Atzeri, Angela; Nieddu, Mariella; Tuberoso, Carlo Ignazio Giovanni; Rescigno, Antonio; Amri, Mohamed; Fattouch, Sami

doi:10.1016/j.indcrop.2016.04.003

Cited by 7 publications

(9 citation statements)

References 34 publications

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“…These compounds have gained importance in recent years due to their high cytotoxic potential against several cancer cells. It was shown that polyphenol-rich extracts from various anatomical parts of Crataegus promoted apoptosis of breast adenocarcinoma (MCF-7) [ 43 ], cervical cancer (HeLa), liver cancer (HepG2), neuroblastoma (IMR-32), colorectal adenocarcinoma (SW480, Coco-2) [ 44 , 45 ], and melanoma (B16F10) [ 21 ]. At the same time, these extracts showed no cytotoxic effects on healthy colon epithelial cells [ 45 ] and normal human peripheral mononuclear cells [ 46 ].…”

Section: Discussionmentioning

confidence: 99%

Polyphenolic Compounds of Crataegus Berry, Leaf, and Flower Extracts Affect Viability and Invasive Potential of Human Glioblastoma Cells

et al. 2021

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Crataegus contains numerous health-promoting compounds that are also proposed to have anti-cancer properties. Herein, we aimed at a contemporaneous evaluation of the effects of polyphenol-rich extracts of berries, leaves, and flowers of six Crataegus species on the viability and invasive potential on the highly aggressive human glioblastoma U87MG cell line. The treatment with the extracts evoked cytotoxic effects, with the strongest in the berry extracts. All extracts not only promoted the apoptosis-related cleavage of poly (ADP-ribose) polymerase 1 (PARP1) but also substantially inhibited the activity of pro-survival kinases, focal adhesion kinase (FAK), and protein kinase B (PKB; also known as Akt), thus indicating the suppression of proliferative and invasive potentials of the examined glioblastoma cells. The qualitative and quantitative characterization of the extracts’ content was also performed and revealed that amongst 37 polyphenolic compounds identified in the examined Crataegus extracts, the majority (29) was detected in berries; the leaf and flower extracts, exerting milder cytotoxic effects, contained only 14 and 13 compounds, respectively. The highest polyphenol content was found in the berries of C. laevigata x rhipidophylla x monogyna, in which flavan-3-ols and phenolic acids predominated. Our results demonstrated that a high content of polyphenolic compounds correlated with the extract cytotoxicity, and especially berries were a valuable source of compounds with anti-cancer potential. This might be a promising option for the development of an effective therapeutic strategy against highly malignant glioblastomas in the future.

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Section: Discussionmentioning

confidence: 99%

Polyphenolic Compounds of Crataegus Berry, Leaf, and Flower Extracts Affect Viability and Invasive Potential of Human Glioblastoma Cells

et al. 2021

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“…Hypolipidemic and antioxidant effects of aqueous and ethanolic extracts of C. pinnatifida fruits were compared in hyperlipidemia rats and obvious differences between extracts were observed, probably due to the existing differences in the content of phenols (Shao et al, 2016). Polyphenolic extracts of C. azarolus and C. monogyna leaves, fruit peel, and pulp, possessing antioxidant activity, protected against thermal-cholesterol degradation while pre-treatment with the extracts preserved liposomes and rat liver-homogenate from oxidative Cu 2+ and Fe 2+ induced damage (Belkhir et al, 2016). Synergic effects and potential mechanisms of action of Astragalus polysaccharides combined with Crataegus flavonoids were studied with diabetic mice.…”

Section: Hypolipidemic Effectsmentioning

confidence: 99%

“…The extract from hawthorn fruit peel (EPHF), rich in phenolic compounds, exhibited dose-dependent cytotoxicity on MCF-7 and SKOV-3 human tumor cell lines with the IC 50 of 2.76 and 80.11 μg/mL, respectively (Wu et al, 2017). Polyphenolic extracts of C. azarolus and C. monogyna leaves, fruit peel, and pulp (0.24-4.8 mg/mL) were cytotoxic to cancer Caco-2 cells, at the same time being nontoxic for differentiated Caco-2 cells (Belkhir et al, 2016). Ethanolic extract of C. microphylla flowers and leaf buds demonstrated cytotoxic and antiproliferative activity against HeLa cell (IC 50 = 0.871 mg/mL) and least cytotoxicity on normal human peripheral mononuclear cells (Bura et al, 2016).…”

Section: Venskutonismentioning

confidence: 99%

Phytochemical composition and bioactivities of hawthorn (Crataegus spp.): review of recent research advances

Venskutonis¹

2018

JFB

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Hawthorn (Crataegus spp.) is one of the most famous plants which has been used as natural medicine and nutraceutical. Its phytochemical composition, bioactive compounds and health benefits have been intensively studied and hawthorn preparations may be recognized as classical natural products for cardiovascular health. Polyphenolic compounds of different hawthorn anatomical parts as well as their extracts have been the focus of a majority of these studies, although various other classes of natural health promoting constituents have also been isolated, identified and characterized. Regardless, numerous published reports have particularly focused on the activity mechanisms which are very important for supporting various health benefits. This review summarizes the most recent studies on hawthorn, mainly published since 2015. Search of different databases indicates that approximately 200 publications, which are relevant to phytochemistry and health benefits of Crataegus spp., have appeared since then, most of them have not been included in the existing reviews.

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“…Our body can counteract the activity of free radicals through endogenous antioxidant mechanisms and the introduction through the diet of exogenous substances [89]. Among the endogenous factors are enzymes such as superoxide dismutase (SOD; EC 1.15.1.1) which catalyzes the dismutation of the superoxide radical (O 2 − ) into either molecular oxygen (O 2 ) or hydrogen peroxide (H 2 O 2 ), catalase (CAT; EC 1.11.1.6), which removes intracellular H 2 O 2 , and reduced glutathione (GSH) [90].…”

Section: Biological Activities Lamium Plantsmentioning

confidence: 99%

Lamium Plants—A Comprehensive Review on Health Benefits and Biological Activities

et al. 2019

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This work is an updated snapshot of Lamium plants and their biological activities. The main features of the plant are described and the components of its essential oils are summarized. The traditional medicinal uses of Lamium plants has been reported. The presence of these chemicals i.e., hydroxycinnamic acids, iridoids, secoiridoids, flavonoids, anthocyanins, phenylpropanoids, phytoecdysteroids, benzoxazinoids, betaine can provide biological activities. After the discussion of antioxidant properties documented for Lamium plants, the biological activities, studied using in vitro models, antimicrobial, antiviral, anti-inflammatory, anti-nociceptive activity, and pain therapy and cytotoxicity and cytoprotective activity are here described and discussed. Finally, targeted examples of in vivo studies are reported.

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Protective effects of azarole polyphenolic extracts against oxidative damage using in vitro biomolecular and cellular models

Cited by 7 publications

References 34 publications

Polyphenolic Compounds of Crataegus Berry, Leaf, and Flower Extracts Affect Viability and Invasive Potential of Human Glioblastoma Cells

Polyphenolic Compounds of Crataegus Berry, Leaf, and Flower Extracts Affect Viability and Invasive Potential of Human Glioblastoma Cells

Phytochemical composition and bioactivities of hawthorn (Crataegus spp.): review of recent research advances

Lamium Plants—A Comprehensive Review on Health Benefits and Biological Activities

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