Protective effects of Annona muricata ethanolic leaf extract against Dimethylnitrosamine (DMN)-Induced Hepatotoxicity

Usunobun, Usunomena

doi:10.9790/3008-09420106

Cited by 3 publications

(4 citation statements)

References 33 publications

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“…In rats administered DMN-alone, the significant increase in total bilirubin level compared to control and extract pre-treated rats gives credence to our previous study where we reported increases in AST, ALT, ALP and GGT (Usunobun, 2014. The abnormal high level of serum total bilirubin indicates liver dysfunction and thus damaged structural integrity of the liver.…”

Section: Discussionsupporting

confidence: 60%

“…However, Annona muricata pre-treatment prior to DMN administration significantly lowered the serum urea and creatinine levels, thus enhancing renal function. In this study, DMN decreased white blood cells WBCs, Platelets, RBCs, PCV, and Hb in DMN treated group compared to control groups similar to our previous study (Usunobun, 2014). However, pre-treatment with 400mg/kg Annona muricata prior to DMN administration significantly (P˂0.05) enhanced the hematological parameters, thus indicating that Annona muricata improve immunity function and decrease inflammation.…”

Section: Discussionsupporting

confidence: 51%

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Effect of Annona muricata pre-treatment on liver synthetic ability, kidney function and hematological parameters in dimethylnitrosamine (DMN)-administered rats

Usunobun

Okolie

2016

IJM

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Background:Dimethylnitrosamine (DMN) is a potent hepatotoxin, carcinogen and mutagen which exerts carcinogenic effects and induces hepatic necrosis in experimental animals through metabolic activation by CYP2E1. This study thus evaluated efficacies of Annona muricata pre-treatment on acute dimethylnitrosamine (DMN)-induced anemia and biochemical alterations in male albino rats. Methods:Four (4) groups of six (6) rats each were used for the study. Group 1 served as control and was untreated, group 2 and 3 were pre-treated with 400mg/kg Annona muricata ethanol leaf extract for one week while group 3 and 4 each received single dose of 20mg/kg DMN (orally) after one week. The rats were sacrificed 48hrs after DMN administration. Results:In rats administered 20mg/kg DMN, toxicty was clearly shown by decreased activities of serum liver synthetic molecules namely total protein, albumin, increased total bilirubin, increased kidney parameters such as creatinine and urea as well as decreased body weight gain. The toxic effect of DMN was also indicated by significantly decreased levels of red blood cells (RBC), hemoglobin (Hb), packed cell volume (PCV), white blood cells (WBC) and platelet count (Plt count). However, in rats pre-treated with 400mg/kg Annona muricata prior to DMN administration, there were significant reversals in the activities of serum liver synthetic molecules, kidney profiles, and hematological parameters when compared to DMN-alone administered rats. Conclusion:Annona muricata pre-treatment exhibited in vivo protective and anti-anemic effects against DMN-induced injury.

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Section: Discussionsupporting

confidence: 60%

Section: Discussionsupporting

confidence: 51%

Effect of Annona muricata pre-treatment on liver synthetic ability, kidney function and hematological parameters in dimethylnitrosamine (DMN)-administered rats

Usunobun

Okolie

2016

IJM

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“…White blood cells play the main role in immune responses. These cells carry out the many tasks required to protect the body against disease-causing microbes and abnormal cells (Lee et al, 2012;Usunobun, 2014). In the present study, DMH increased the WBCs, lymphocytes and granulocytes in DMH alone treated group compared to control and extract pretreated and post treated groups.…”

Section: Olude O M; Chukwu O Jsupporting

confidence: 55%

Protective Effect of Ethanol Extract of Annona Muricata Leaves on Hematological Profile, Histology and Oxidative Stress in 1,2-Dimethylhydrazine-induced Colorectal Carcinogenesis in Rats

Olude¹,

Omoregie²,

Chukwu³

2023

jasem

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Cancer starts when cells in the body start to grow out of control (malignant growth). Colorectal cancer starts in the colon or the rectum. Annona Muricata is a medicinal plant and is considered as a basis for health preservation and care worldwide. Colorectal cancer has reached epidemic proportions and is considered as a serious health problem, therefore treatment and cure of this disease is of great clinical importance. 1,2 dimethylhydrazine (DMH) is a potent carcinogen for colon cancer in rats. In this study, rats were randomly allocated into six groups: control, extract alone, DMH, extract and DMH together, extract before DMH, DMH before extract. DMH was injected subcutaneously at 25mg/1000g body weight once a week for 16 weeks, while the ethanol extract of Annona muricata leaves (EEAML) were administered to rats orally at 120mg/1000g. After sacrifice, colon and liver sections were dissected out for histological and some biochemical analysis. Blood was also collected in EDTA bottles for hematological assays. The group injected with DMH (subcutaneously) and orally administered leaves extract of A. Muricata simultaneously showed similar results with the rats fed with normal feed, as there was absence of polyps in the rats’ colon when compared with those injected with DMH alone for 16 weeks. Malondialdehyde level of rats injected with DMH were significantly increased (p ˂ 0.05) compared to the control. Hematology indices and caspase 3 activity show the protective effects of EEAML against DMH.

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“…This current study shows that ethanol extract of Annona muricata leaves have both combined anticarcinogenic and antioxidant activities that inhibited colon carcinogenesis and simultaneously suppress oxidative stress. The efficacy of EEAML on DMH induced colon carcinogenesis inhibition is likely boosted by its antioxidative action and presence of some compounds like acetogenins, flavonoids, tannins, proanthocyanidin, phenols, ferric acid reducing antioxidant potential of EEAML and DPPH radical scavenging activity of EEAML (Olude et al, 2020;Usunobun et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

Antioxidant potential of ethanol extract of Annona muricata leaves and its inhibitory effect on lipid peroxidation in 1,2-dimethylhydrazine induced colon carcinogenesis

Olude,

Omoregie

2023

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Annona muricata, as it is scientifically called but known in most parts of Nigeria as soursop. A fruit tree with various therapeutic uses, the plant belongs to the family of Annonaceae and has several medicinal properties. The medicinal properties of Annona muricata are well known, and they can be used to treat a variety of illnesses and conditions. Effects of ethanol extract of Annona muricata leaves (EEAML) on the enzymatic antioxidants of the kidney and colon in Wistar rats induced with 1, 2 Dimethylhydrazine (DMH) at 25 mg/kg bodyweight s.c. were investigated in this study. Thirty-six male Wistar rats weighing 110–170g were acclimated for two weeks and randomized into six groups (six per group). Group A (control), Group B (extract; 120 mg/kg body weight of ethanolic extract of A. muricata), Group C (DMH only), Group D (DMH + EXTRACT), Group E (pretreatment), and Group F (posttreatment). The extract was administered daily via oral gavage, while the DMH was given subcutaneously at a dosage of 25 mg/kg bodyweight. This study reveals the carcinogenic effect of DMH induced oxidative stress were ameliorated by the administration of this leaf extract of EEAML at a dose of 120mg/kg b.w. The colon and kidney homogenates of rats administered EEAML (co-treated, pretreatment and post- treatment) showed increased enzymatic antioxidant; glutathione peroxidase, catalase activities and SOD activities. But a reduction in malondialdehyde levels was observed. These findings validate the use of Annona muricata in traditional medicine by showing that the ethanolic extract of the leaves can reduce DMH-induced oxidative stress and alter the enzymatic antioxidant profile in colon carcinogenesis.

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Protective effects of Annona muricata ethanolic leaf extract against Dimethylnitrosamine (DMN)-Induced Hepatotoxicity

Cited by 3 publications

References 33 publications

Effect of Annona muricata pre-treatment on liver synthetic ability, kidney function and hematological parameters in dimethylnitrosamine (DMN)-administered rats

Effect of Annona muricata pre-treatment on liver synthetic ability, kidney function and hematological parameters in dimethylnitrosamine (DMN)-administered rats

Protective Effect of Ethanol Extract of Annona Muricata Leaves on Hematological Profile, Histology and Oxidative Stress in 1,2-Dimethylhydrazine-induced Colorectal Carcinogenesis in Rats

Antioxidant potential of ethanol extract of Annona muricata leaves and its inhibitory effect on lipid peroxidation in 1,2-dimethylhydrazine induced colon carcinogenesis

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