Protective effects of an extract from Citrus bergamia against inflammatory injury in interferon-gamma and histamine exposed human keratinocytes

Graziano, Adriana Carol Eleonora; Cardile, Venera; Crascì, Lucia; Caggia, Silvia; Dugo, Paola; Bonina, Francesco; Panico, Annamaria

doi:10.1016/j.lfs.2012.04.043

Cited by 45 publications

(33 citation statements)

References 45 publications

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“…Moreover, these data are also in accord with Graziano et al. who reported the anti‐inflammatory effect of naringin, neohesperidin, apigenin, and neoeriocitrin on inflammatory response in keratinocytes. Interestingly our results highlight the importance of the contemporary presence of all these compounds in Citrus Bergamia because the contemporary presence of all the four compounds together increase the anti‐inflammatory effect with respect to the single compounds alone being significant versus LPS alone also at the lowest concentration (Fig.…”

Section: Resultssupporting

confidence: 90%

Multidimensional preparative liquid chromatography to isolate flavonoids from bergamot juice and evaluation of their anti-inflammatory potential

et al. 2015

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Important objectives of a high-performance liquid chromatography preparative process are: purity of products isolated, yield, and throughput. The multidimensional preparative liquid chromatography method used in this work was developed mainly to increase the throughput; moreover purity and yield are increased thanks to the automated collection of the molecules based on the intensity of a signal generated from the mass spectrometer detector, in this way only a specific product can be targeted. This preparative system allowed, in few analyses both in the first and second dimensions, the isolation of eight pure compounds present at very different concentration in the original sample with high purity (>95%) and yield, which showed how the system is efficient and versatile. Pure molecules were used to validate the analytical method and to test the anti-inflammatory and antiproliferative potential of flavonoids. The contemporary presence, in bergamot juice, of all the flavonoids together increases the anti-inflammatory effect with respect to the single compound alone.

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Section: Resultssupporting

confidence: 90%

Multidimensional preparative liquid chromatography to isolate flavonoids from bergamot juice and evaluation of their anti-inflammatory potential

et al. 2015

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“…). It has been reported that ROS act as key intermediates that are directly involved in the histamine‐mediated inflammation pathway . In this study, we revealed that a pathological dose of histamine could promote the formation of intracellular ROS and stimulate inflammation‐related cytokines in A7r5 cells (Fig.…”

Section: Discussionmentioning

confidence: 58%

Diallyl sulfide as a potential dietary agent to reduce TNF‐α‐ and histamine‐induced proinflammatory responses in A7r5 cells

Weng

Jhang

et al. 2014

Molecular Nutrition Food Res

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“…To Cell viability was evaluated by the colorimetric 3-(4,5dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) assay (MTT test) as reported 14 . Briefly, 20 ml of 0.5% MTT in phosphate saline buffer (PBS) were added to each microwell and cells were incubated for 4 h at 37 C in a humidified 95% air/ 5% CO 2 mixture.…”

Section: Mtt Assaymentioning

confidence: 99%

A study on liposomal encapsulation of a lipophilic prodrug of LHRH

Pignatello

Musumeci

Graziano

et al. 2015

Pharmaceutical Development and Technology

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This study aimed at evaluating whether derivatization of luteinizing hormone-releasing hormone (LHRH) peptide with an amphiphilic lipoamino acid moiety could allow, along with other technological and/or pharmacokinetic advantages, to improve its encapsulation in liposomes, potentially driving its further body distribution and cellular uptake. Experimental data confirmed that a lipophilic derivative of LHRH was efficiently incorporated in various liposomal systems, differing in lipid composition and surface charge, and obtained using different methods of production. Incubation of liposomes, loaded with a fluorescent derivative of the LHRH prodrug, with NCTC keratinocytes or Caco-2 cell cultures showed that the carriers can be rapidly internalized. Conversely, the internalization of the free prodrug occurred only at very high concentrations.

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Protective effects of an extract from Citrus bergamia against inflammatory injury in interferon-gamma and histamine exposed human keratinocytes

Cited by 45 publications

References 45 publications

Multidimensional preparative liquid chromatography to isolate flavonoids from bergamot juice and evaluation of their anti-inflammatory potential

Multidimensional preparative liquid chromatography to isolate flavonoids from bergamot juice and evaluation of their anti-inflammatory potential

Diallyl sulfide as a potential dietary agent to reduce TNF‐α‐ and histamine‐induced proinflammatory responses in A7r5 cells

A study on liposomal encapsulation of a lipophilic prodrug of LHRH

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