Protective effects of alfalfa saponins on oxidative stress-induced apoptotic cells

Cui, Yalei; Liu, Boshuai; Sun, Xiao Wei; Li, Zidan; Chen, Yanyan; Guo, Zhiguo; Liu, Hua; Li, Defeng; Wang, Chengzhang; Zhu, Xiaoyan; Ya, Shi

doi:10.1039/d0fo01797c

Cited by 20 publications

(13 citation statements)

References 31 publications

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“…In this regard, plant extracts showed remarkable anti-inflammatory properties thanks to the presence of multiple molecules [66,75]. At the same time, other authors highlighted the anti-inflammatory actions of single molecules purified from plant extracts [5,36,38]. Further studies will be needed to evaluate whether the phytocomplex of GP shows a greater anti-inflammatory power than any single molecule present in the same phytocomplex.…”

Section: Discussionmentioning

confidence: 99%

“…Like all secondary metabolites produced by plants, the saponin content is also influenced by various biotic stimuli such as parasite attacks, pathogenic infections, the mutualistic symbiosis of plants with rhizobium bacteria and mycorrhizal fungi [35]. Saponins are functional components of many plant-based drugs and popular medicines and have shown an important function in improving the inflammatory, immune and anticancer response and in reducing cholesterol [36,37]. Furthermore, saponins showed good activity in cellular protection from ischemia and hypoxia [38].…”

Section: Saponinsmentioning

confidence: 99%

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Protective Effects of Gynostemma pentaphyllum (var. Ginpent) against Lipopolysaccharide-Induced Inflammation and Motor Alteration in Mice

et al. 2021

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Gynostemma pentaphyllum (var. Ginpent) (GP) is a variety of Cucurbit with anti-inflammatory and antioxidant effects in patients. In this manuscript, the main components present in the dry extract of GP have been identified using Ultra High Performance Liquid Chromatography quadrupole-time-of-flight mass spectrometry (UHPLC/Q-TOF-MS). In addition, the anti-inflammatory action of GP was evaluated in animal models with acute peripheral inflammation and motor alteration induced by lipopolysaccharide. The results showed that GP dry extract is rich in secondary metabolites with potential antioxidant and anti-inflammatory properties. We found that the treatment with GP induced a recovery of motor function measured with the rotarod test and pole test, and a reduction in inflammatory cytokines such as interleukin-1β and interleukin-6 measured with the ELISA test. The data collected in this study on the effects of GP in in vivo models may help integrate the therapeutic strategies of inflammatory-based disorders.

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Section: Discussionmentioning

confidence: 99%

Section: Saponinsmentioning

confidence: 99%

Protective Effects of Gynostemma pentaphyllum (var. Ginpent) against Lipopolysaccharide-Induced Inflammation and Motor Alteration in Mice

et al. 2021

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“…Concentration-dependent and time-dependent assays were conducted to determine the cell viability of cells decreased by about half after the treatment with 300 μmol/L H 2 O 2 for 24 h compared with the control group. The results indicated that the median lethal concentration of H 2 O 2 in IPEC-J2 cells was 300 μmol/L, which was higher than the median lethal concentration (150 μmol/L) of H 2 O 2 in IEC-6 cells, indicating IPEC-J2 cells were much more tolerant to H 2 O 2 than IEC-6 cells [ 16 ]. For the suitable concentration and appropriate time of IPEC-J2 oxidative stress stimulation, cells treated with 300 μmol/L H 2 O 2 for 24 h were determined as the oxidative stress model of this experiment.…”

Section: Discussionmentioning

confidence: 99%

“…Intestinal mucosal epithelial cells are important functional cells of the intestine which play a key role in digestive enzymes’ secretion, nutrients’ digestion, absorption, transport, stress response and immune barrier [ 14 , 15 ]. The previous study has shown the antioxidant effects of alfalfa saponins on rat intestinal epithelial cells (IEC-6 cell line) [ 16 ]. However, IEC-6 is a cell line from rat, one kind of model animal, whose digestive tube construction is very different from that of swine [ 17 ].…”

Section: Introductionmentioning

confidence: 99%

Alfalfa saponins inhibit oxidative stress-induced cell apoptosis through the MAPK signaling pathway

Cui

Zhu

et al. 2021

Redox Report

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Background: Oxidative stress could seriously affect the growth performance of piglets. As natural extracts of Alfalfa (Medicago sativa) , alfalfa saponins have been shown to function as antioxidants in piglets in vivo . However, few studies have investigated the effects and mechanism of alfalfa saponins against oxidative stress in piglet cells in vitro . In the current study, piglets’ small intestinal epithelial cell line (IPEC-J2) was explored to investigate the protective effects of alfalfa saponins on injured cells induced by H 2 O 2 . Methods: To investigate the effects and mechanism of alfalfa saponins against oxidative stress in piglet cells, the cell viability, activity of antioxidant enzymes, LDH and the amount of MDA were detected in H 2 O 2 -treated cells after the cells were pre-incubated with alfalfa saponins. The mechanism of alfalfa saponins against H 2 O 2 -induced oxidative cell damage was explored by detecting the expression of mitochondrial apoptosis-related proteins. Furthermore, the signaling pathway of alfalfa saponins in IPEC-J2 cells under oxidative stress was also investigated. Results: The results indicated that alfalfa saponins could rescue cell viability, elevate the activity of antioxidant enzymes and down-regulate the activity of LDH and the amount of MDA in H 2 O 2 -induced cells. Conclusion: Alfalfa saponins could inhibit oxidative stress-induced cell mitochondrial apoptosis through the MAPK signaling pathway, thereby providing a new method for improving antioxidant stress ability by means of nutritional regulation.

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“…These compounds are responsible for antidiabetic, anti-obesity, antioxidant, as well as many other biological activities. Other non-phenols such as the saponin component of alfalfa play key biological functions in the body [ 23 ]. Saponins and their derivatives such as prosapogenins and sapogenins have been reported to exert a high antimicrobial activity against yeasts and bacterial strains [ 24 ].…”

Section: Phytochemicals In Selected Edible Plant Sproutsmentioning

confidence: 99%

Edible Plant Sprouts: Health Benefits, Trends, and Opportunities for Novel Exploration

et al. 2021

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The consumption of plant sprouts as part of human day-to-day diets is gradually increasing, and their health benefit is attracting interest across multiple disciplines. The purpose of this review was to (a) critically evaluate the phytochemicals in selected sprouts (alfalfa, buckwheat, broccoli, and red cabbage), (b) describe the health benefits of sprouts, (c) assess the recent advances in sprout production, (d) rigorously evaluate their safety, and (e) suggest directions that merit special consideration for further novel research on sprouts. Young shoots are characterized by high levels of health-benefitting phytochemicals. Their utility as functional ingredients have been extensively described. Tremendous advances in the production and safety of sprouts have been made over the recent past and numerous reports have appeared in mainstream scientific journals describing their nutritional and medicinal properties. However, subjects such as application of sprouted seed flours in processed products, utilizing sprouts as leads in the synthesis of nanoparticles, and assessing the dynamics of a relationship between sprouts and gut health require special attention for future clinical exploration. Sprouting is an effective strategy allowing manipulation of phytochemicals in seeds to improve their health benefits.

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Protective effects of alfalfa saponins on oxidative stress-induced apoptotic cells

Abstract: As it is known, alfalfa saponin can be used as a feed additive in the pig’s diet. And the addition of alfalfa saponin to the pig’s diet could improve animal...

Cited by 20 publications

References 31 publications

Protective Effects of Gynostemma pentaphyllum (var. Ginpent) against Lipopolysaccharide-Induced Inflammation and Motor Alteration in Mice

Protective Effects of Gynostemma pentaphyllum (var. Ginpent) against Lipopolysaccharide-Induced Inflammation and Motor Alteration in Mice

Alfalfa saponins inhibit oxidative stress-induced cell apoptosis through the MAPK signaling pathway

Edible Plant Sprouts: Health Benefits, Trends, and Opportunities for Novel Exploration

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