Protective effects of abdominal electroacupuncture on oxidative stress and inflammation due to testis torsion/detorsion in rats

Abstract

Conception, design, intellectual and scientific content of the study, critical revision, final approval of the manuscript. ABSTRACT PURPOSE:To evaluate the effects of acupuncture (Ac) and electroacupuncture (EAc) on oxidative stress and inflammation in testis torsion/detorsion (T/D) model in rats. METHODS:Thirty male Wistar rats were randomized into five groups. G1 Group (Sham) served as control. The remaining groups were submitted to spermatic cord torsion (720°) for 3 hours, followed by detorsion and reperfusion for 4 hours. Before detorsion G3, G4 and G5 rats were treated with Ac, EAc 2Hz and EAc 10 Hz, respectively, applied to acupoint Gulai (S-29) bilaterally under anesthesia for 5 minutes. Next, the testes were detorsioned and reperfused for 4 hours. Afterwards, blood samples and the right testis were collected for biochemical assays: reduced Glutathione (GSH), Malonaldehyde (MDA), Myeloperoxidase (MPO). RESULTS:EAc stimulation (2 and 10 Hz) promoted significant increase in concentrations of GSH in plasma and testis of G4-G5 rats, compared with G1. There was significant increase of tissue MDA in groups G4-G5 and plasma MDA in all groups, compared with G1.There was a significant reduction in MPO activity in groups G4-G5 compared with G1. CONCLUSION:Electroacupuncture stimulation (2 and 10 Hz) attenuates oxidative stress and inflammatory response in rats subjected to testicular torsion/detorsion. Key words: Spermatic Cord Torsion. Ischemia. Reperfusion Injury. Electroacupuncture. Rats. Protective effects of abdominal electroacupuncture on oxidative stress and inflammation due to testis torsion/detorsion in rats

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