Protective effects and molecular mechanisms of baicalein on thioacetamide-induced toxicity in zebrafish larvae

Zhang, June; Deng, Yunyun; Cheng, Bo; Huang, Yong; Meng, Yunlong; Zhong, Keyuan; Xiong, Gaofeng; Guo, Jing; Liu, Yi; Lu, Huiqiang

doi:10.1016/j.chemosphere.2020.127038

Cited by 21 publications

(13 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Zebrafish larvae were stimulated with 0.4 mg/ml TAA for 72 h to establish the NAFLD model successfully, which manifested by hepatocytes swelling and steatosis according to previous research ( Zhang et al, 2020 ). Based on this model, the protective effect of limonin on NAFLD was evaluated.…”

Section: Resultsmentioning

confidence: 99%

Limonin Alleviates Non-alcoholic Fatty Liver Disease by Reducing Lipid Accumulation, Suppressing Inflammation and Oxidative Stress

Yang

Lin

et al. 2022

Front. Pharmacol.

View full text Add to dashboard Cite

Non-alcoholic fatty liver disease (NAFLD) is the most common cause of chronic liver disease and continues to rise in the worldwide. Limonin is a triterpenoid compound widely found in the fruits of citrus plants with a wide range of pharmacological effects, including anti-cancer, anti-inflammation, anti-viral, anti-oxidation and liver protection properties. However, the potential molecular mechanism of limonin on NAFLD in zebrafish remains unknown. In this study, zebrafish larvae were exposed to thioacetamide to establish an NAFLD model and the larvae were treated with limonin for 72 h simultaneously. The human liver cell line was stimulated with lipid mixture and meanwhile incubated with limonin for 24 h. The results showed that Limonin significantly reduced the accumulation of lipid droplets in the liver and down-regulated the levels of lipogenic transcription factors FASN and SREBP1 in NAFLD. Limonin suppressed macrophages infiltration and the down-regulated the relative expression levels of the pro-inflammatory factors IL-6, IL-1β and TNF-α secreted by macrophages. Besides, limonin could reversed the reduction of glutathione and the accumulation of reactive oxygen species through up-regulating NRF2/HO-1 signaling pathway in the liver. In conclusion, this study revealed that limonin has a protective effect on NAFLD due to its resistance to lipid deposition as well as antioxidant and anti-inflammatory actions.

show abstract

Section: Resultsmentioning

confidence: 99%

Limonin Alleviates Non-alcoholic Fatty Liver Disease by Reducing Lipid Accumulation, Suppressing Inflammation and Oxidative Stress

Yang

Lin

et al. 2022

Front. Pharmacol.

View full text Add to dashboard Cite

show abstract

“…Specially, zebrafish completes primary liver morphogenesis in 48 h postfertilization (hpf) and full liver function in 72 hpf. The quick liver development and its high conservativeness made zebrafish a universal model organism for hepatotoxicity and hepatoprotection screening, basic understanding of acquired liver diseases [ 35 ]. Hereby, to further investigate antioxidant activities of LFP-a1 and its potential application in NAFLD treatment, larval zebrafish were exposed to exogenous addition of LFP-a1 in TAA-induced NAFLD model.…”

Section: Resultsmentioning

confidence: 99%

Hepatoprotection of Lycii Fructus Polysaccharide against Oxidative Stress in Hepatocytes and Larval Zebrafish

Zhang

et al. 2021

Oxidative Medicine and Cellular Longevity

View full text Add to dashboard Cite

Scavenging of oxidative stress by antioxidants may provide a therapeutic strategy for nonalcoholic fatty liver disease (NAFLD). Increasing evidence is supporting the potential application of natural resourced polysaccharides as promising prevention or treatment strategies against NAFLD. In the current study, an acidic heteropolysaccharide, LFP-a1, was isolated and purified from Lycii fructus with successively hot water refluxing extraction, alcohol precipitation, protein removal, and DEAE-52 cellulose chromatographic separation. LFP-a1 was a complicated structured polysaccharide with an average MW of 4.74 × 104 Da and composed of 6 monosaccharides and 1 uronic acid. Preexposure of LFP-a1 could increase the cell viability and reverse the abnormal oxidative stress though inhibition of mitochondrial-mediated apoptotic pathway and correction of cell cycle progression against H2O2 hepatoxicity in NAFLD model L02 cells. Consistently, in vivo study in thioacetamide- (TAA-) induced NAFLD model zebrafish larvae showed LFP-a1 preserved the liver integrity and alleviated TAA-induced oxidative stress through downregulation of abnormal apoptosis. These observations indicated the hepatoprotective activity of LFP-a1, which may be applied for the prevention or treatment of NAFLD or other oxidative stress-related diseases.

show abstract

“…From this behavioral analysis associated with the indices of histopathological alterations (section 3.4), it is possible to conclude that BASF does not have an acute toxic effect on animals, since the avonoid BA is capable of producing protective effects against induced toxicity, already proven in zebra sh larvae (Zhang et al 2020a).…”

Section: Dpph Antioxidant Potentialmentioning

confidence: 92%

Anti-inflammatory potential of Baicalein combined with silk fibroin protein in a Zebrafish model (Danio rerio)

Holanda

Ribeiro

Sánchez-Ortíz

et al. 2022

Preprint

View full text Add to dashboard Cite

Baicalein (BA) is a flavonoid with wide-ranging pharmacological activity. However, its biological evaluation is hampered by its low solubility in aqueous medium, making forms of incorporation that improve its solubility necessary. In the present study, BA was combined with a solution of silk fibroin protein (SF), a biomaterial used as a drug carrier, to evaluate the anti-inflammatory potential of this combination, in vivo, in an experimental model, zebrafish (Danio rerio). Baicalein-Silk fibroin (BASF) improved the DPPH (2,2‐diphenyl‐1‐picryl‐hydrazyl‐hydrate) free radical scavenging rate (95%) in comparison with BA in solution. The acute toxicity study and histopathological analysis in zebrafish showed that BASF showed low cytotoxic potential up to a dose of 2.000 mg/kg. The use of BA in combination with SF enhanced the anti-inflammatory effect of flavonoids by inducing inflammatory peritoneal edema through carrageenan and achieved 77,6% inhibition of abdominal edema at a dose of 75 mg/kg. The results showed that the BASF, significantly increases the bioavailability and therapeutic effect of flavonoids and several results observed in this study may help in the development of new drugs.

show abstract

Protective effects and molecular mechanisms of baicalein on thioacetamide-induced toxicity in zebrafish larvae

Cited by 21 publications

References 35 publications

Limonin Alleviates Non-alcoholic Fatty Liver Disease by Reducing Lipid Accumulation, Suppressing Inflammation and Oxidative Stress

Limonin Alleviates Non-alcoholic Fatty Liver Disease by Reducing Lipid Accumulation, Suppressing Inflammation and Oxidative Stress

Hepatoprotection of Lycii Fructus Polysaccharide against Oxidative Stress in Hepatocytes and Larval Zebrafish

Anti-inflammatory potential of Baicalein combined with silk fibroin protein in a Zebrafish model (Danio rerio)

Contact Info

Product

Resources

About