Protective Effects and Molecular Mechanism of Total Flavonoids from Lycium Barbarum Leaves on Photoaged Human Dermal Fibroblasts

Abstract

Objective. To investigate the effects and corresponding mechanisms of total flavonoids (TFL) from Lycium barbarum leaves on photoaged human dermal fibroblasts (HDFs). Methods. Crude TFL was extracted with 70% ethanol, and a Rutin standard curve was drawn using the sodium nitrite-aluminum nitrate-sodium hydroxide colorimetry method to calculate its yield and mass concentration. After that, the photoaging HDFs model was established by UVA combined with 8-MOP. CCK-8 was performed to assess the influence of TFL on the proliferation of HDFs and photoaging HDFs. β-galactosidase (SA-β-gal) staining and activity assays were performed to evaluate the activity of SA-β-gal and the rate of SA-β-gal-positive cells in HDFs cells. The level of skin ECM proteins and oxidative stress-related substances in HDFs cells of each group was determined by ELISA and biochemical detection, respectively. Apoptosis of HDFs in each group was assessed by flow cytometry. The expressions of MAPK signaling pathway-related proteins in HDFs were detected by western blot. Results. The yield rate of TFL extracted by 70% ethanol was 41.9%, and its purity rate was 34.6%. TFL at 25, 50, and 100 μg/mL was able to greatly promote the proliferation of HDFs. A photoaged HDFs model was successfully constructed by combining UVA irradiation at 9 J/cm2 and 8-MOP at 50 mg/L. TFL treatment could significantly inhibit apoptosis, SA-β-gal-positive cell staining rate, SA-β-gal activity, lactate dehydrogenase (LDH) leakage, and malondialdehyde (MDA) content in photoaged HDFs. Further, TFL increased the proliferative activity, superoxide dismutase (SOD) activity, catalase (CAT) activity, type I collagen (Col I), hydroxyproline (HYP), and hyaluronic acid (HA) level of photoaged HDFs in a dose-dependent manner. Additional experiments suggested that TFL played a protective role by downregulating MAPK signaling pathway activity in photoaged HDFs cells. Conclusion. TFL could inhibit oxidative stress and apoptosis, promote cell proliferation and the level of ECM-related component proteins, and participate in antiphotoaging in a concentration-dependent manner. The protective role of TFL in photoaged HDFs might be related to its inhibition of MAPK signaling pathways.

OsCM regulates rice defence system in response to UV light supplemented with drought stress

“…A recent study showed that, SOD and MDA are negatively correlated, which means that increasing the SOD activity reduces MDA content (Song et al . 2022).…”

“…A possible reason for this negative correlation is the accumulation of flavonoids. Flavonoids increases cell proliferation, SOD, and CAT activity, which inhibits oxidative stress (Song et al 2022). As SOD is present in almost all plants that generate activated oxygen, it has been predicted that SOD has a central role in oxidative (El-Beltagi & Mohamed 2013).…”

“…As SOD is present in almost all plants that generate activated oxygen, it has been predicted that SOD has a central role in oxidative (El-Beltagi & Mohamed 2013). A recent study showed that, SOD and MDA are negatively correlated, which means that increasing the SOD activity reduces MDA content (Song et al 2022).…”

Heat-Killed Lacticaseibacillus paracasei Ameliorated UVB-Induced Oxidative Damage and Photoaging and Its Underlying Mechanisms

“…A similar result was found in the study on flavonoids, one kind of metabolites in heat-killed probiotics. Song et al (2022) reported that total flavonoids from lycium barbarum leaves could decrease SA-β-gal-positive cell staining rate, thereby protecting against UVA-induced photoaging in human dermal fibroblasts [ 71 ].…”

Optimization of Extraction of Compound Flavonoids from Chinese Herbal Medicines Based on Quantification Theory and Evaluation of Their Antioxidant Activity

“…After vacuum filtration, the hydroethanolic extracts were concentrated at 40 °C by a rotary evaporator (SY-2000, Yarong Technology and Science Inc., Shanghai, China), then freeze-dried at −20 °C using a lyophilizer (FD-1A, Boyikang Experimental Instrument Co., Ltd., Beijing, China), and finally ground into powder (termed CFs). e sodium nitrite-aluminum nitrate method [19] was utilized to determine the content of CFs with a UV-Vis spectrophotometer (UV-2500PC, Shimadzu Co., Kyoto, Japan). e absorbance was measured at 510 nm, and the standard curve with reference substance of rutin was Y � 7.1105X-0.004 (R 2 � 0.9994), where Y is the absorbance and X is the concentration of rutin.…”