Protective effects and mechanisms of Baicalin and octreotide on renal injury of rats with severe acute pancreatitis

Abstract

AIM:To i n v e s t i g a t e t h e p r o t e c t i v e e f fe c t s a n d mechanisms of Baicalin and octreotide on renal injury of rats with severe acute pancreatitis (SAP). METHODS:One hundred and eighty SD rats were randomly assigned to the model group, Baicalin-treated group, octreotide-treated group and sham operation group. The mortality, plasma endotoxin level, contents of blood urea nitrogen (BUN), creatinine (CREA), phospholipase A2 (PLA2), nitrogen monoxide (NO), tumor necrosis factor (TNF)-α, IL-6 and endothelin-1 (ET-1) in serum, expression levels of renal Bax and Bcl-2 protein, apoptotic indexes and pathological changes of kidney were observed at 3, 6 and 12 h after operation. RESULTS:The renal pathological changes were milder in treated group than in model group. The survival at 12 h and renal apoptotic indexes at 6 h were significantly (P < 0.05) higher in treated group than in model group [66.67% vs 100%; 0.00 (0.02)% and 0.00 (0.04)% vs 0.00 (0.00)%, respectively]. The serum CREA content was markedly lower in octreotide-treated group than in model group at 3 h and 6 h (P < 0.01, 29.200 ± 5.710 μmol/L vs 38.400 ± 11.344 μmol/L; P < 0.05, 33.533 ± 10.106 μmol/L vs 45.154 ± 17.435 μmol/L, respectively).The expression level of renal Bax protein was not significantly different between model group and treated groups at all time points. The expression level of renal Bcl-2 protein was lower in Baicalin-treated group than in model group at 6 h [P < 0.001, 0.00 (0.00) grade score vs 3.00 (3.00) grade score]. The Bcl-2 expression level was lower in octreotide-treated group than in model group at 6 h and 12 h [P < 0.05, 0.00 (0.00) grade score vs 3.00 (3.00) grade score; 0.00 (0.00) grade score vs 0.00 (1.25) grade score, respectively]. The serum NO contents were lower in treated groups than in model group at 3 h and 12 h [P < 0.05, 57.50 (22.50) and 52.50 (15.00) μmol/L vs 65.00 (7.50) μmol/L; P < 0.01, 57.50 (27.50) and 45.00 (12.50) μmol/L vs 74.10 (26.15) μmol/L, respectively]. The plasma endotoxin content and serum BUN content (at 6 h and 12 h) were lower in treated groups than in model group. The contents of IL-6, ET-1, TNF-α (at 6 h) and PLA2 (at 6 h and 12 h) were lower in treated groups than in model group [P < 0.001, 3.031 (0.870) Key words: S e ve re a c u t e p a n c re a t i t i s ; B a i c a l i n ; Octreotide; Renal injury; Rats; Tissue microarrays

Acute kidney injury following acute pancreatitis: A review

“…In one experimental animal study, Zhang XP et al reported some protective effects and the mechanism of Baicalin and Octreotide on renal injury in rats with SAP. The authors showed from histopathological tissue microarray sample of kidney inhibition of inflammatory mediators, decreasing the expression level of Bcl-2 protein and enhancing the apoptosis-promoting effect of Bax dimmer to induce apoptosis of renal tubular cells 104 .…”

Study of dexamethasone, baicalin and octreotide on brain injury of rats with severe acute pancreatitis

“…After systemic inflammatory response syndrome (SIRS) was introduced, the important role of excessive inflammatory reaction in the pathogenesis of SAP has been gradually recognized [31][32][33][34]. The symptoms, such as disorientation, clouding of consciousness, as well as agitation accompanied by delusions and hallucinations, present in acute pancreatitis is called pancreatic encephalopathy.…”

“…Furthermore, baicalin also has some protective effects on the nervous system [16][17][18][19]. These observations suggest that baicalin may be used to treat SAP [20][21][22][23].…”

Influence of dexamethasone on the expression levels of P-selectin protein in multiple organs of rats with severe acute pancreatitis

“…The standards for the pathological severity scores of pancreas, liver, kidney, and lung have been shown in our series of reports [8,21,22]. The standard for the pathological severity score for the spleen was as follows: a score of 0 was assigned if spleen structure was normal; 1 if necrosis in follicular center was seen; 2 if blood sinus expansion and arteriolosclerosis were observed; 3 if necrosis in follicular center, blood sinus expansion, and arteriolosclerosis were observed.…”