Protective Effect on Lipids Profile of Lion’s Foot (Alchemilla Vulgaris ) Leaves against Ccl4 Toxicity and its Fortified to Guava and Mango Pulp

Abstract

Lion's foot (Alchemilla vulgaris L.) which is widely known as lady's mantle, bear's foot or lion's foot is traditionally used due to their tannin contents for the treatment of inflammations, diarrhea, wound healing and inhibit cardiovascular diseases and cystic fibrosis. The present work aims to high light the reverse effect of dried lion's foot (Alchemilla vulgaris) leaves and its water and ethanol extracts at various ratios against the toxicity of CCl 4 on serum lipids profile that may occur on albino rats. Also, preparation of guava and mango pulp that fortified with lion's foot powder and its water and ethanol extracts as an available product rich in bioactive component to help hyperlipidemic patients. Fifty-six male albino rats were used in this experiment. These rats were divided into eight main groups (seven rats of each) and were fed on diets for 45 days as follows: Group 1: Negative control group was fed on basal diet. Forty nine rats were fed on basal diet and treated with CCl 4 subcutaneous injection to induce toxicity, then divided into 7 groups form group 2 to group 8. Group 2: Positive control group will be fed on basal diet till final experiment. Groups 3:8 as the same of the second group with (50 and 100 ppm ethanol extract, 50 and 100 ppm water extract, 1% and 2% dry leaves powder respectively) daily orally. Moreover, guava and mango pulps were classified to 7 groups as follows: control sample, and the samples (2 to 7) were fortified with lion's foot as follows ethanol extract 50ppm and 100ppm , water extract 50ppm and 100ppm, 1% and 2% dried leaves for sensory evaluation. All these samples were organoleptically evaluated for general appearance, color, flavor, taste, consistency and overall acceptability. Results: The results revealed that treatment with lion's foot leaves and its ethanol and water extracts caused significant decreased levels of serum lipid profile of total cholesterol, triglycerides, total lipids, LDL-C and VLDL-C induced by CCl 4 and enhancement HDL-C in all groups. The best result of lipid profile was in groups treatment with the high level 2% dried lion foot followed 1 % dried lion's foot and 100ppm ethanol extract. These results due to the antioxidants content in Lion's foot as polyphenols, flavonoids, tannins and saponins (359.65, 183.10, 150.64 and 296.32, respectively). The best sample of fortified guava pulp was 1% dried leaves. While, the best result of mango pulp was in the samples fortified with 50ppm ethanol extract, water extracts (50 and 100 ppm) and 1% dried leaves. Conclusion:This study showed that the fortification of Alchemilla vulgaris L. can be proposed to protect the toxicity induced by CCl 4 in rats, also to help inhibit the improvement cardiovascular diseases and cystic fibrosis.