Protective Effect of Yinhua Miyanling Tablet on Lipopolysaccharide‐Induced Inflammation through Suppression of NLRP3/Caspase‐1 Inflammasome in Human Peripheral Blood Mononuclear Cells

Abstract

Yinhua Miyanling Tablet (YMT), the Chinese formula, has long been administrated in clinical practice for the treatment of acute pyelonephritis and acute urocystitis. In the current study, we aimed to investigate the anti-inflammatory effect of YMT in vitro and to evaluate the association between anti-inflammation and innate immune response. Human peripheral blood mononuclear cells (PBMCs) were isolated using Ficoll density gradient centrifugation and then were stimulated by Lipopolysaccharide (LPS). The differential gene expression of inflammation-related genes after drug administration was assessed using PCR array, and the protein levels of differential genes were measured by ELISA and Western blot. The result showed that YMT significantly inhibited the expression of NLRP3, Caspase-1, and the downstream cytokine IL-1β and suppressed the production of inflammatory mediators TNF-α, IL-6, IL-10, and MCP-1 in a dose-dependent manner compared to the LPS group (P < 0.01). The finding indicated that YMT exhibited anti-inflammatory effect in vitro by suppressing the NLRP3/Caspase-1 inflammasome, and that may have therapeutic potential for the treatment of inflammatory diseases.

Anti‐inflammatory character of Phelligridin D modulates periodontal regeneration in lipopolysaccharide‐induced human periodontal ligament cells

“…Oral inflammatory diseases are mainly caused by bacteria infections to the dentin or root canal . LPS, the main bacteria toxoid, induces inflammatory cytokines IL‐1, ‐6, ‐8 and TNF‐α and inflammatory molecules, matrix metalloproteinases (MMP‐9 and MMP‐2) in human peripheral blood mononuclear cells, dental pulp cells and gingival fibroblasts . In this study, a low concentration of phelligridin D , eg, <5 μmol/L, did not have cytotoxicity in HDPLCs.…”

N6-Methyladenosine Modification of Fatty Acid Amide Hydrolase Messenger RNA in Circular RNA STAG1–Regulated Astrocyte Dysfunction and Depressive-like Behaviors

“…In addition to binding with enzymes/proteins, circRNAs exert their biological functions via other mechanisms. Accumulating evidence indicates that circRNA HRCR (heart-related circRNA) binds miR-223 to regulate cardiac hypertrophy and heart failure (38), and another circRNA, SRY (sex-determining region Y), acts as a sponge for miR-138 (13). In addition, some circRNAs have demonstrated excess ability to encode proteins or peptides (39).…”

The Role of Regulatory T Cell in Nontypeable Haemophilus influenzae-Induced Acute Exacerbation of Chronic Obstructive Pulmonary Disease

“…Lung tissue from mice was ground into homogenates at the condition of 4°C for protein detection [ 26 ]. Total protein was extracted via a Total Protein Extraction Kit (Invent Biotechnologies Inc., USA) according to the manufacturer's protocol.…”