Protective effect of wax apple (Syzygium samarangense (Blume) Merr. &amp; L.M. Perry) against streptozotocin-induced pancreatic ß-cell damage in diabetic rats

Khamchan, Aree; Paseephol, Tatdao; Hanchang, Wanthanee

doi:10.1016/j.biopha.2018.09.072

Cited by 38 publications

(32 citation statements)

References 47 publications

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“…Endogenous antioxidant kinases in the mitochondria, such as SOD, catalase (CAT), and GSH-PX, protect cells from free radical damage. ROS and RNS can directly damage β cells, es-pecially by disrupting the cell mitochondrial structure to cause the loss of antioxidant-related enzymes and promote β cell apoptosis [17][18][19]. In addition, several studies have shown that in T2D patients and animal models, the expression of inflammatory cytokines (IL-1β, IL-6, and TNF-α) was upregulated, and inhibiting the expression of proinflammatory factors significantly reduces the apoptosis level of islet β cells and improves pancreatic function [20].…”

Section: Discussionmentioning

confidence: 99%

Protection of Curcumin against Streptozocin-Induced Pancreatic Cell Destruction in T2D Rats

Shuntian

Zhou

et al. 2019

Planta Med

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As a kind of traditional Chinese medicine extract, curcumin has been proven to be effective in inhibiting inflammation and apoptosis in pancreatic islet β cells in the streptozotocin-induced diabetes mellitus rat model, although the underlying mechanism has not yet been clarified. To examine the effect of curcumin on inflammation and apoptosis in pancreatic islet β cells, we established a type 2 diabetes rat model by feeding the animals a high-fat diet and intraperitoneally injecting streptozotocin. The curcumin was administered by intraperitoneal injection. The rat body weight, fasting blood glucose, intraperitoneal glucose tolerance tests, and insulin tolerance tests were recorded and analyzed. Hematoxylin and eosin staining was used for morphological analysis, and a TUNEL assay was performed to detect the apoptotic cells. The expression levels of proteins related to oxidative stress, inflammation and apoptosis were detected by Western blotting and ELISA. Curcumin administration significantly decreased fasting blood glucose and promoted recovery of pancreas function in type 2 diabetes rats. In curcumin-treated rats, the pancreatic tissue destruction and apoptosis index were reduced. The expression of IL-1β, IL-6, TNF-α, caspase-3, Bax, and malondialdehyde were significantly reduced, and Bcl-2, superoxide dismutase 2, and glutathione peroxidase were significantly increased. Curcumin inhibited the expression of phosphorylated JNK and NF-κB proteins to block the RAGE/JNK/NF-κB signaling pathway. In conclusion, these results indicate that curcumin blocks the phosphorylation of JNK and NF-κB protein to inhibit this signaling pathway, thereby further inhibiting inflammation and apoptosis in pancreatic islet β cells. Curcumin has potential value for the treatment of diabetes.

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Section: Discussionmentioning

confidence: 99%

Protection of Curcumin against Streptozocin-Induced Pancreatic Cell Destruction in T2D Rats

Shuntian

Zhou

et al. 2019

Planta Med

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“…HOMA‐IR and HOMA‐β are models used for estimating the degree of insulin resistance and β‐cell function. They are considered an effective biomarkers for the prediction of insulin sensitivity (Khamchan, Paseephol, & Hanchang, 2017; Xia et al, 2019). In our study the HOMA‐IR index of diabetic rats was significantly and dose dependently upregulated by treatment with T. triandra (100 and 400 mg/kg) indicating the alleviation of insulin resistance .…”

Section: Discussionmentioning

confidence: 99%

Tiliacora triandra extract possesses antidiabetic effects in high fat diet/streptozotocin‐induced diabetes in rats

2020

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The antidiabetic properties of Tiliacora triandra ethanol extract in diabetic rats induced with high‐fat diet (HFD)/streptozotocin (STZ) was investigated. Rats were fed with HFD for 4 weeks to induced insulin resistance, and thereafter administered with 35 mg/kg of STZ to induce diabetes. Diabetic rats received 100 and 400 mg/kg of T. triandra daily for 30 days. The body weight, blood glucose level, food and fluid intake were monitored. Furthermore, biochemical and histological assessment was performed to evaluate the hypoglycemic effect of the extract in the treated rats. T. triandra significantly decreased the blood glucose level, increased the body weight and insulin secretion. Furthermore, T. triandra attenuated hyperlipidemia, improved liver and kidney functions of treated diabetic rats. Thus, T. triandra could effectively attenuate diabetes and it complications. Practical applications Tiliacora triandra is a common vegetable consumed in Thailand and Laos. It is traditionally employed in the treatment of fever, cancer, malaria, and diabetes. The extract from the aerial part was investigated for its antidiabetic properties. The results obtained provides important pharmacological information that supports the use of T. triandra in management of diabetes.

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“…High ROS levels can cause OS and may directly contribute to tissue cellular damage related to the expression of caspase-3. It has been reported that OS induced apoptosis and one of the important proteins in internal and external pathway of apoptosis was caspase-3 [33]. Therefore, caspase-3 expression could increase in cells when exposed to toxic chemicals, and the overexpression of caspase-3 was in accordance with the damage to the tissues.…”

Section: Discussionmentioning

confidence: 94%

“…The results indicated that OS events were independent of caspase-3 activity and other molecular mechanisms may be involved. There is controversy over the activation of caspase pathway associated with the toxicity of NPs [22,33]. Silver nanoparticles (AgNPs) have been shown to induce OS in human colon cancer cells through increased LPO and reduced GSH levels with the induction of mitochondria-involved apoptosis via the elevated levels of Bcl and caspase-3 proteins [34].…”

Section: Discussionmentioning

confidence: 99%

Toxicity of microwave-assisted biosynthesized zinc nanoparticles in mice: a preliminary study

Salimi

Rahimi

Forootanfar

et al. 2019

Artificial Cells, Nanomedicine, and Biotechnology

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This study was designed to describe the oral acute and subacute toxicities and underlying toxicological mechanisms of biogenic Zn NPs in mice. The Zn NPs were prepared by a green microwave-assisted synthesis method in the presence of Lavandula vera leaf extract. Determination of median lethal dose (LD 50) of Zn NPs and the subacute toxicity after 14 days of exposure was performed as a measurement of substance toxicity through general toxicological, hematological, serum, and histopathological investigations. The western blotting was used to determine the cleaved-caspase-3 expression in the sampled tissues. Flame atomic absorption spectrophotometer (AAS) was applied to estimate the Zn levels in tissues. The SEM analyses revealed that the biogenic Zn NPs were spherical-shaped with the size range of 30-80 nm. The LD 50 value above 5 g/kg indicated that biogenic Zn NPs could be classified as nontoxic chemicals. In subacute toxicity, no significant differences were found in the body weight as well as hematological and oxidative stress (OS) biomarkers after exposure to Zn NPs at the dose of 1 g/kg in comparison to the control. The AAS results indicated that Zn NPs were mainly distributed in the testis, liver, and brain. The findings of histology images of Zn NPs at the dose of 1 g/kg were similar to those of the control. Furthermore, no significant differences were observed in cleaved-caspase-3 expression after exposure to Zn NPs at the dose of 5 g/kg. The results demonstrated that changes in the OS were not related to caspase pathway and the no-observed-adverse-effect level (NOAEL) dose of biogenic Zn NPs in 14-days subacute toxicity study was lower than 1 g/kg.

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Protective effect of wax apple (Syzygium samarangense (Blume) Merr. & L.M. Perry) against streptozotocin-induced pancreatic ß-cell damage in diabetic rats

Cited by 38 publications

References 47 publications

Protection of Curcumin against Streptozocin-Induced Pancreatic Cell Destruction in T2D Rats

Protection of Curcumin against Streptozocin-Induced Pancreatic Cell Destruction in T2D Rats

Tiliacora triandra extract possesses antidiabetic effects in high fat diet/streptozotocin‐induced diabetes in rats

Toxicity of microwave-assisted biosynthesized zinc nanoparticles in mice: a preliminary study

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