Protective Effect of Vaccination with <i>Toxoplasma</i> Lysate Antigen and CpG as an Adjuvant against <i>Toxoplasma gondii</i> in Susceptible C57BL/6 Mice

El-Malky, Mohamed; Lu, Shaohong; Kumagai, Toru; Yabu, Yoshisada; Noureldin, Mohamed S.; Saudy, Niveen; Maruyama, Haruhiko; Ohta, Nobuhiro

doi:10.1111/j.1348-0421.2005.tb03656.x

Cited by 39 publications

(18 citation statements)

References 34 publications

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“…El‐Malky et al. (28) and Kringel et al. (40) reported CpG ODN to be an effective adjuvant for use in vaccination against toxoplasmosis in mice and pigs.…”

Section: Discussionmentioning

confidence: 99%

“…Hitherto, the role of CpG ODN as an adjuvant in vaccination against T. gondii infection has been debatable. El-Malky et al (28) and Kringel et al (40) reported CpG ODN to be an effective adjuvant for use in vaccination against toxoplasmosis in mice and pigs. However, Saavedra et al (41) found that CpG ODN had only a limited role in the protection against T. gondii in mice.…”

Section: Discussionmentioning

confidence: 99%

“…El‐Malky et al. (28) has shown that CpG ODN provides a stable and effective adjuvant for use in vaccination against toxoplasmosis.…”

Section: Introductionmentioning

confidence: 99%

“…Co-delivery of plasmid encoding GM-CSF has been shown to enhance antibody level to target antigens in sheep (27). El-Malky et al (28) has shown that CpG ODN provides a stable and effective adjuvant for use in vaccination against toxoplasmosis.…”

Section: Introductionmentioning

confidence: 99%

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Immunological response of sheep to injections of plasmids encoding Toxoplasma gondii SAG1 and ROP1 genes

Olędzka

McFarlane

et al. 2010

Parasite Immunology

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Infection with the intracellular protozoan parasite Toxoplasma gondii (T. gondii) causes health problems to both humans and livestock and has a large economic impact worldwide. The immune response in sheep following infection with T. gondii was evaluated using six different combinations of plasmid DNA, recombinant antigen and adjuvant. Sheep were generally vaccinated twice by intramuscular injection with plasmid DNA containing gene sequences for either the surface antigen (SAG1) or the rhoptry protein (ROP1) of T. gondii. Two of the groups injected with plasmid DNA SAG1 were boosted with recombinant protein (SAG1). We investigated the efficacy of including oligodeoxynucleotides (ODN) that contain CG motifs (CpG) and the gene coding for ovine granulocyte-macrophage colony stimulating factor (GM-CSF) as potential adjuvants. Administration of the plasmid encoding the ROP1 gene significantly enhanced both IFN-gamma production from peripheral blood cells when cultured in vitro with Toxoplasma antigen, and ROP1-specific IgG1 and IgG2 antibody levels present in serum. However, injection with SAG1 did not stimulate IFN-gamma production. These results indicate the potential of ROP1, given as plasmid DNA, as a potential vaccine candidate to protect sheep against T. gondii infection.

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“…El‐Malky et al. (28) and Kringel et al. (40) reported CpG ODN to be an effective adjuvant for use in vaccination against toxoplasmosis in mice and pigs.…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

“…El‐Malky et al. (28) has shown that CpG ODN provides a stable and effective adjuvant for use in vaccination against toxoplasmosis.…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Immunological response of sheep to injections of plasmids encoding Toxoplasma gondii SAG1 and ROP1 genes

Olędzka

McFarlane

et al. 2010

Parasite Immunology

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show abstract

“…with either low 1 × 10 2 or high (1 × 10 3 or 1 × 10 5 ) doses of viable RH (25). Total tachyzoite lysate antigen was prepared from in vitro HFF cultures of RH as described (26, 27). For cytokine assays mice were infected i.p.…”

Section: Methodsmentioning

confidence: 99%

Cell-Mediated Immunity toToxoplasma gondiiDevelops Primarily by Local Th1 Host Immune Responses in the Absence of Parasite Replication

Gigley

Fox

Bzik

2009

The Journal of Immunology

120

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A single inoculation of mice with the live, attenuated Toxoplasma gondii uracil auxotroph strain cps1-1 induces long-lasting immunity against lethal challenge with hypervirulent strain RH. The mechanism for this robust immunity in the absence of parasite replication has not been addressed. The mechanism of long-lasting immunity, the importance of route of immunization, cellular recruitment to the site of infection, and local and systemic inflammation were evaluated. Our results show that infection with cps1-1 elicits long-lasting CD8+ T cell- mediated immunity. We show that immunization with cps1-1-infected dendritic cells elicits long-lasting immunity. Intraperitoneal infection with cps1-1 induced a rapid influx of GR1+ neutrophils and two stages of GR1+CD68+ inflammatory monocyte infiltration into the site of inoculation. CD19+ B cells and CD3+ T cells steadily increase for 8 days after infection. CD8+ T cells were rapidly recruited to the site of infection and increased faster than CD4+ T cells. Surprisingly, cps1-1 infection induced high systemic levels of bioactive IL-12p70 and a very low level and transient systemic IFN-γ. Furthermore, we show significant levels of these inflammatory cytokines were locally produced at the site of cps1-1 inoculation. These findings offer new insight into immunological mechanisms and local host responses to a non-replicating type I parasite infection associated with development of long-lasting immunity to Toxoplasma gondii.

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Evaluation of the adjuvant effect of pidotimod on the immune protection induced by UV-attenuated Toxoplasma gondii in mouse models

Zhao

Huang

et al. 2013

Parasitol Res

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The current anti-Toxoplasma gondii drugs have many shortcomings and effective vaccines against T. gondii may contribute to the control of this pathogen. Pidotimod is a synthetic substance capable of stimulating both cellular and humoral immunity. To investigate the possible adjuvant effect of pidotimod on the immune response to T. gondii in Kunming mice induced by ultraviolet-attenuated T. gondii (UV-T.g), in this study, mice were immunized intraperitoneal (i.p.) with UV-T.g or UV-T.g co-administered with pidotimod (UV-T.g + PT). After infection or challenge by i.p. injection of 10(2) RH tachyzoites, the animal survival rate, parasite burden in peritoneal lavage fluids, liver histopathology, the level of serum anti-toxoplasma IgG antibody, and the mRNA expressions of IL-2, IFN-γ, and TNF-α from spleen analyzed using real-time PCR, were compared among different groups. The results showed that, compared with infected controls, infected mice treated with pidotimod had significantly increased survival rate and extended survival time, decreased parasite burden, improved liver histopathology, increased level of anti-toxoplasma IgG antibody, and increased mRNA expressions of Th1-type cytokine (IL-2, IFN-γ, and TNF-α) (P < 0.01), while mice vaccinated with UV-T.g and then challenged had even significantly increased survival rate and extended survival time, decreased parasite burden, improved liver histopathology, and increased mRNA expressions of Th1-type cytokines (IL-2, IFN-γ, and TNF-α) (P < 0.01); furthermore, vaccinated mice co-administered with pidotimod had even more lower parasite burden, milder liver histopathology, and higher levels of Th1-type cytokine and anti-toxoplasma IgG antibody (P < 0.01). Our data demonstrated that pidotimod in vivo could promote strong and specific humoral and cellular immune response to T. gondii challenge infection when co-administered with UV-attenuated T. gondii. It suggests that pidotimod may have the potential to be used as an effective vaccine adjuvant.

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Protective Effect of Vaccination with Toxoplasma Lysate Antigen and CpG as an Adjuvant against Toxoplasma gondii in Susceptible C57BL/6 Mice

Cited by 39 publications

References 34 publications

Immunological response of sheep to injections of plasmids encoding Toxoplasma gondii SAG1 and ROP1 genes

Immunological response of sheep to injections of plasmids encoding Toxoplasma gondii SAG1 and ROP1 genes

Cell-Mediated Immunity toToxoplasma gondiiDevelops Primarily by Local Th1 Host Immune Responses in the Absence of Parasite Replication

Evaluation of the adjuvant effect of pidotimod on the immune protection induced by UV-attenuated Toxoplasma gondii in mouse models

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