Protective effect of urapidil on testicular torsion–detorsion injury in rats

Meštrović, Jakov; Pogorelić, Zenon; Drmić-Hofman, Irena; Vilović, Katarina; Todorić, Davor; Popović, Marijana

doi:10.1007/s00595-016-1388-3

Cited by 28 publications

(27 citation statements)

References 31 publications

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“…Also, in a study on effect of intraperitoneal injection of the Nifedipine (100 mg/kg) on testicular torsion-detorsion injury in rats, Mestrovic et al 34 reported Nifedipine significantly decreased MDA and increased SOD and GPx levels which our results was in agreement to this report. On protective effect of Urapidil on testicular torsion Meštrovic´ et al 35 reported intraperitoneal injection of the 10 ml/kg of the Urapidil 30 min before detorsion significantly significantly decreased MDA and increased SOD and GPx levels on testicular torsion-detorsion injury in rats. Also, Ozbek et al 36 revealed intraperitoneal injection of the Apocynin (20 mg/kg) significantly increased SOD, GPx and CAT and decreased MDA levels in 4h torsion followed by 1h detorsion rats.…”

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confidence: 99%

Effects of folic acid administration on testicular ischemia/reperfusion injury in rats

et al. 2017

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1 8-Experimental SurgeryActa Cir Bras. 2017;32(9):755-766 Abstract Purpose: To determine the effect of folic acid (FA) on experimental testicular ischemia/reperfusion (I/R) in rats. Methods: Sixty male Wistar rats were randomly divided into 6 groups. The control group received physiologic saline orally. The sham-operated group received physiologic saline orally then exposed to midline laparotomy without clamping the IR. The I/R rats received oral gavage of the saline then subjected to 1h ischemia /24h reperfusion, period. In folic acid (2mg/ kg+IR) rats received oral gavage of the FA (2mg/kg) then subjected to 1h I/24h R. groups 5-6 received FA (5 and 10 mg/kg), then subjected to 1 h I/24 h, respectively. At the end of the study, semen samples were collected for spermatozoa characteristics. The left testis was removed for histological analysis and superoxide dismutase (SOD), malondialdehyde (MDA) and glutathione peroxidase (GPx) measurement. Results: Spermatozoa mobility, mortality (%) significantly decreased in I/R group (P<0.05). Dose dependent increase observed on spermatozoa mobility, mortality (%) using different levels of the FA (2, 5 and 10 mg/kg) treated rat (P<0.05). Tissue MDA levels significantly increased in I/R rat (P<0.05) while FA (2, 5 and 10mg/kg) in a dose dependent manner decreased I/R-induced MDA (P<0.05). Experimental I/R significantly decreased SOD and GPx activity (P<0.05). Administration of the FA (2, 5 and 10mg/kg) significantly increased tissue SOD and GPx activity in I/R rat (P<0.05). Seminiferous tubules degenerated and loss of spermatogenesis with few spermatocytes was observed in degenerated testis tubules in I/R rat. Orally administration of the FA (5 and 10 mg/kg) improved testis characteristics with few normal seminiferous tubules and spermatocyte in seminiferous tubules in experimental I/Rinduced rat. Conclusion:The treatment of folic acid had a benefit effect against ischemia-reperfusion.

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confidence: 99%

Effects of folic acid administration on testicular ischemia/reperfusion injury in rats

et al. 2017

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“…In an experimental study conducted by Mestrovic J. et al, the rats treated with nifedipine had a significant decrease in malondialdehyde level and apoptosis and had significant increases in superoxide dismutase and glutathione peroxidase activities in ipsilateral testes compared with those not treated with nifedipin (Mestrovic, 2014). Also in another study, Meštrović et al (2017) shown protective effect of urapidil on testicular torsion-detorsion injury in rats.…”

Section: Xue Et Al Investigated the Effect Of Gradual Low-flow Repermentioning

confidence: 82%

“…In another study, IMA level was significantly higher in both the 2-hr acute torsion and 2-month chronic torsion groups, and it was stated that IMA level might have a predictive value for fertility in addition to its use in the diagnosis of testicular torsion (Mentese et al, 2012). Also in another study, Meštrović et al (2017) shown protective effect of urapidil on testicular torsion-detorsion injury in rats. Pre-orchiectomy IMA levels were significantly higher in the sham group than in the other groups.…”

Section: Xue Et Al Investigated the Effect Of Gradual Low-flow Repermentioning

confidence: 90%

Predictive value of ischaemia‐modified albumin in spermatogenesis in an experimental testicular torsion model

et al. 2019

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Our aim was to measure the ability of ischaemia-modified albumin (IMA) to predict testicular histopathological damage in the testes of rats with short-and long-term ischaemia using experimental testicular torsion and subsequent reperfusion via detorsion.21 Wistar Albino rats were randomized into three groups. The sham group was subjected to a mid-scrotal incision only. The 4-and 8-hr T/D (Torsion/Detorsion) groups were subjected to left testicular torsion by twisting the testes by 720 degrees counterclockwise. 2 cc venous blood samples were taken from the sham group after the mid-scrotal incision, and from the 4-and 8-hr T/D groups after 4 and 8 hr respectively. After that, the 4-and 8-hr T/D groups were subjected to detorsion.Two days later, orchiectomy was performed. Ischaemia-modified albumin levels were significantly different among the groups at 48 hr prior to orchiectomy (reperfusion; p = .003). Based on the results of the paired comparisons, it was found that IMA levels of the sham group were significantly higher than those of the 4-and 8-hr T/D groups (p = .002 and .009 respectively). Our study has showed that IMA may be used to predict ischaemia/reperfusion injury, which is another complication that may occur following detorsion in testicular torsion. K E Y W O R D Sexperimental testicular torsion, ischaemia, ischaemia-modified albumin, rats, reperfusion, spermatogenesis

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“…Diclofenac, an anti-inflammatory agent, exerted similar effects, as evidenced by elevated levels of DNA fragments detected using the TUNEL assay to measure the levels of germ-cell apoptosis (Mogilner et al, 2006). Bozlu et al (2009) When given before the detorsion, Nifedipine, a calcium channel blocker vasodilator and Urapidil, a α1-adrenoceptor antagonist sympatholytic prevented I/R damage in the testicular tissue cells (Meštrović et al, 2014(Meštrović et al, , 2017. Furthermore, apocynin, an NADPH oxidase inhibitor, reduced the degree of lipid peroxidation, induced the formation of antioxidant substances, and reversed the I/R injury in the rat testis cells (Ozbek et al, 2015;Şener et al, 2015).…”

Section: Caspase-3 Normally Exists In the Cytosolic Fraction Of Cells Asmentioning

confidence: 98%

“…Antioxidants such as N-acetylcysteine (Cay et al, 2006) and garlic extract (Unsal et al, 2006) have been used to lower the amounts of overgenerated ROS. Bozlu et al (2009) When given before the detorsion, Nifedipine, a calcium channel blocker vasodilator and Urapidil, a α1-adrenoceptor antagonist sympatholytic prevented I/R damage in the testicular tissue cells (Meštrović et al, 2014(Meštrović et al, , 2017. Diclofenac, an anti-inflammatory agent, exerted similar effects, as evidenced by elevated levels of DNA fragments detected using the TUNEL assay to measure the levels of germ-cell apoptosis (Mogilner et al, 2006).…”

Section: Caspase-3 Normally Exists In the Cytosolic Fraction Of Cells Asmentioning

confidence: 99%

Metformin decreases testicular damages following ischaemia/reperfusion injury in rats

et al. 2019

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The effects of metformin on a testicular torsion injury in adolescent rat testis after I/R were evaluated in the present study. Forty adolescent rats were divided into five groups with eight rats per group: a control group; a sham‐operated group; an ischaemia group, where torsion was applied for 4 hr and testis was examined immediately after detorsion; an I/R group, where torsion was applied for 4 hr and the testis was examined 4 hr after detorsion; and an I/R + M group, where the metformin (300 mg/kg) administration was added to the identical procedures used for the I/R group. Spermatogenesis, basal membrane integrity and cleaved caspase‐3 expression were assessed. The I/R + M group had a significantly higher Johnsen score than the I/R group (7.9 ± 0.1 vs. 7.5 ± 0.2; p < .001; F‐value = 14.2). Failure of basal membrane integrity was highest in the ischaemia group (45 ± 5) compared to the other groups (control group, 20 ± 5; sham‐operated group, 16.6 ± 2.8), but not different between the I/R + M (31.6 ± 12.5) and the I/R groups (25 ± 3.5). Cleaved caspase‐3 expression was highest in the ischaemia group (73.5 ± 0.7), and significantly lower in the I/R + M group (33.4 ± 0.9) than the I/R group (58.5 ± 0.2; p < .05; F‐value = 7.6). Metformin decreases testicular damage by exerting protection against the harmful effects of I/R on spermatogenesis and alleviating apoptosis in adolescent rat testis.

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Protective effect of urapidil on testicular torsion–detorsion injury in rats

Abstract: The administration of urapidil before detorsion prevents ischemia/reperfusion cellular damage in testicular tissue. LMWH was not found to have a beneficial effect on testicular T/D injury in rats.

Cited by 28 publications

References 31 publications

Effects of folic acid administration on testicular ischemia/reperfusion injury in rats

Effects of folic acid administration on testicular ischemia/reperfusion injury in rats

Predictive value of ischaemia‐modified albumin in spermatogenesis in an experimental testicular torsion model

Metformin decreases testicular damages following ischaemia/reperfusion injury in rats

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