Protective Effect of Tuna Bioactive Peptide on Dextran Sulfate Sodium-Induced Colitis in Mice

Abstract

Bioactive peptides isolated from marine organisms have shown to have potential anti-inflammatory effects. This study aimed to investigate the intestinal protection effect of low molecular peptides (Mw < 1 kDa) produced through enzymatic hydrolysis of tuna processing waste (tuna bioactive peptides (TBP)) on dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) in BALB/c mice. Here, we randomly divided twenty-four male BALB/c mice into four groups: (i) normal (untreated), (ii) DSS-induced model colitis, (iii) low dose TBP+DSS-treated (200 mg/kg/d), and (iv) high dose TBP+DSS-treated groups (500 mg/kg/d). The results showed that TBP significantly reduced mice weight loss and improved morphological and pathological characteristics of colon tissues. In addition, it increased the activities of antioxidant enzymes (SOD and GSH-Px) and decreased inflammatory factors (LPS, IL-6, and TNF-α) expression. TBP increased the gene expression levels of some tight junction (TJ) proteins. Moreover, TBP increased the short-chain fatty acids (SCFAs) levels and the diversity and imbalance of intestinal flora. Therefore, TBP plays some protective roles in the intestinal tract by enhancing antioxidant and anti-inflammatory abilities of the body, improving the intestinal barrier and metabolic abnormalities, and adjusting intestinal flora imbalance.

Oyster (Crassostrea gigas) Extract Attenuates Dextran Sulfate Sodium-Induced Acute Experimental Colitis by Improving Gut Microbiota and Short-Chain Fatty Acids Compositions in Mice

“…Other researchers have also reported that it is possible to induce a mouse model of acute experimental colitis models using methods similar to those reported in this study [39,40]. A series of studies have shown that food components have favorable ameliorative effects on acute colitis [8][9][10][11][12]. We conducted the first study on the attenuation of acute experimental colitis by OE.…”

“…These alternative therapies are of great interest because of their low side effects [7]. Among these, algal oil [8], propolis extract [9], peptides derived from tuna [10], taurine [11], and astragalin [12] have been reported to exert protective effects against experimental colitis induced by dextran sulfate sodium (DSS) in mice. Thus, the potential exists for IBD patients to have a greater variety of substances that are effective, in the form of alternative treatments.…”

Bioactive peptides from nuts: A review

“…The pecan protein isolate (PPI) hydrolysate showed differential antioxidant activities after fractionation by ultrafiltration through cut‐off membranes with molecular weights of 10, 5, and 3 kDa, where the highest antioxidant effect was observed in the fraction containing the lowest molecular weight peptides (<3 kDa) (Hu et al ., 2018). This enhancement in biological activity can be attributed to a lower steric hindrance and a reduction in repulsion between peptides (Nwachukwu & Aluko, 2019; Sun & Udenigwe, 2020; Xiang et al ., 2021). Likewise, these low molecular weight peptides show better absorption efficiency across biological membranes than larger amino acid sequences (Xiang et al ., 2021).…”

Fish and Shellfish-Derived Anti-Inflammatory Protein Products: Properties and Mechanisms

“…Anti-inflammatory protein products are often short, LMW peptides [ 43 , 52 , 63 ]. This is fortuitous because small peptides are more readily absorbed by the body, and this allows the possibility that the anti-inflammatory peptides may be utilized as orally administered therapeutics [ 3 , 64 ]. LMW protein products are more likely to cross the intestinal barrier intact, thus increasing the possibility that they can exert anti-inflammatory action in the body [ 34 , 48 , 65 ].…”