Protective Effect of Triphlorethol-A from Ecklonia cava against Ionizing Radiation in vitro

Abstract

We studied the cytoprotective effect of triphlorethol-A against gamma-ray radiation-induced oxidative stress. In this study, hydrogen peroxide, which is a reactive oxygen species (ROS), was detected using 2',7'-dichlorodihydrofluorescein diacetate (DCF-DA) assay. Triphlorethol-A reduced intracellular hydrogen peroxide generated by gamma-ray radiation. This compound provided protection against radiation-induced membrane lipid peroxidation and cellular DNA damage which are the main targets of radiation-induced damage. Triphlorethol-A protected the cell viability damaged by the radiation through inhibition of apoptosis. Triphlorethol-A reduced the expression of bax and activated caspase 3 induced by radiation, but recovered the expression of bcl-2 decreased by radiation. Taken together, the results suggest that triphlorethol-A protects cells against oxidative damage induced by radiation through reducing ROS.

Triphlorethol-A Improves the Non-Homologous End Joining and Base-Excision Repair Capacity Impaired by Formaldehyde

“…In addition, triphlorethol-A protected against cellular damage from ionizing radiation via inhibition of apoptosis (Kang et al 2006). Triphlorethol-A induced the antioxidant enzyme heme oxygenase-1 via activation of the NF-E2-related factor 2 transcription factor ).…”

Radioprotective effect of geraniin via the inhibition of apoptosis triggered by γ-radiation-induced oxidative stress

“…Thus, the elimination of the free radical species can act as a target in the search for radioprotective compounds. To date, several natural products have been shown to protect cells against radiation-induced damage by virtue of their antioxidant properties (Kang et al 2006). The present study showed that geraniin, a tannin isolated from N. tetragona Georgi var., effectively protected cells against radiation-induced damage via a mechanism involving the regulation of cellular antioxidant enzyme activity, by inhibition of oxidative stress and free radical scavenging.…”

“…Cells were exposed to γ-ray from a 60 Co γ-ray source (MDS Nordion C-188 standard source, Jeju National University, Jeju, Korea) at a dose rate of 1.5 Gy/min (Kang et al 2006). …”

“…Extensive studies have been conducted to evaluate the potential beneficial effects of natural phytochemicals in radiorecovery and the protection of normal tissue during exposure to radiation (Petrovic et al 2008). Increased understanding of the interrelationship between oxygen effect and radiation exposure have led to a rational application of natural antioxidants Landauer 2000, 2003;Kang et al 2006;Hosseinimehr 2007).…”

Inhibition of Lipid Peroxidation Induced by Ultraviolet Radiation by Crude Phlorotannis Isolated from Brown Algae <i>Sargassum hystrix v. buxifolium</i> C. Agardh

“…This mainly occurs in biomembranes, where the content of unsaturated fatty acids is relatively high. This leads to the destruction of cellular membranes, and is related to the development of many chronic diseases such as aging and cancer [2][3]. Other studies have measured several markers of lipid peroxidation in the human body such as the thiobarbituric acid reactive substances (TBARS), ethylene (ethene) and pentane in the breath gas, lipid hydroperoxides, and aldehydes such as malonaldehyde (MDA) [4].…”