Protective effect of the ultra-filtration extract from Xin Mai Jia on human aortic smooth muscle cell injury induced by hydrogen peroxide

Wan, Jia; Yin, Yulong; Sun, Ruili; Pan, Guopin; Li, Peng; Jia, Yanjie; G, Wan; Liu, Zhangsuo

doi:10.3892/etm.2013.1365

Cited by 8 publications

(6 citation statements)

References 27 publications

(19 reference statements)

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“…By using gene expression array, we identified several signaling pathways related to NO, FOXO, apoptosis, cell cycle, AMPK, MAPK, NF-kB, and PPAR, which were affected by XMJ in endothelial cells. We choose NO signaling as a candidate because NO signaling was in the pecking order among these signaling, consistent with our previous studies that XMJ produces the anti-inflammatory and antioxidant effects in HUVECs by increasing NO productions 19 26 27 . This is also highly related to clinical investigations that Chinese medicine has globally recognized to improve health conditions by activating NO signaling 28 .…”

Section: Discussionmentioning

confidence: 91%

Traditional Chinese medicine xin-mai-jia recouples endothelial nitric oxide synthase to prevent atherosclerosis in vivo

Yin

Zhu

Wan

et al. 2017

Sci Rep

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Endothelial dysfunction, which is caused by endothelial nitric oxide synthase (eNOS) uncoupling, is an initial step in atherosclerosis. This study was designed to explore whether Chinese medicine xin-mai-jia (XMJ) recouples eNOS to exert anti-atherosclerotic effects. Pretreatment of XMJ (25, 50, 100 μg/ml) for 30 minutes concentration-dependently activated eNOS, improved cell viabilities, increased NO generations, and reduced ROS productions in human umbilical vein endothelial cells incubated with H2O2 for 2 hours, accompanied with restoration of BH4. Importantly, these protective effects produced by XMJ were abolished by eNOS inhibitor L-NAME or specific eNOS siRNA in H2O2-treated cells. In ex vivo experiments, exposure of isolated aortic rings from rats to H2O2 for 6 hours dramatically impaired acetylcholine-induced vasorelaxation, reduced NO levels and increased ROS productions, which were ablated by XMJ in concentration-dependent manner. In vivo analysis indicated that administration of XMJ (0.6, 2.0, 6.0 g/kg/d) for 12 weeks remarkably recoupled eNOS and reduced the size of carotid atherosclerotic plaque in rats feeding with high fat diet plus balloon injury. In conclusion, XMJ recouples eNOS to prevent the growth of atherosclerosis in rats. Clinically, XMJ is potentially considered as a medicine to treat patients with atherosclerosis.

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Section: Discussionmentioning

confidence: 91%

Traditional Chinese medicine xin-mai-jia recouples endothelial nitric oxide synthase to prevent atherosclerosis in vivo

Yin

Zhu

Wan

et al. 2017

Sci Rep

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“…NO is the catalytic product of NOS that causes the vasodilation response ( 16 , 17 ). Considering that NO is a gas, directly measuring NO levels is not easy; therefore, reflecting the biological effects of NO is usually performed through the indirect study of NOS ( 18 – 20 ).…”

Section: Discussionmentioning

confidence: 99%

“…A previous study has shown that XMJ can alleviate cardiovascular and cerebrovascular diseases and decrease blood lipid levels ( 17 ). XMJ has clear anti-inflammatory and antioxidant effects and significantly inhibits the proliferation and migration of human aortic smooth muscle cells ( 18 ). Although the effect of XMJ is satisfactory, the exact mechanism of its anti-arteriosclerotic action has not been confirmed.…”

Section: Introductionmentioning

confidence: 99%

Effects and mechanism of Xin Mai Jia in a rabbit model of atherosclerosis

Zhao

Jia

et al. 2015

Experimental and Therapeutic Medicine

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The aim of this study was to investigate the protective effects of Xin Mai Jia (XMJ) on atherosclerosis (AS) in rabbits and to explore the underlying mechanisms in order to provide experimental evidence for the clinical application of XMJ. An intraperitoneal injection of vitamin D3, combined with a high-fat diet and sacculus injury, was utilized to establish the AS rabbit model. Following the oral administration of lovastatin, Zhibituo and different dosages of XMJ, respectively, blood was drawn from each rabbit for the detection of blood rheological indicators, such as serum lipids. The pathological changes in the right common carotid artery were observed. Vascular function experiments and the expression detection of common carotid artery-related proteins by immunohistochemistry were conducted. XMJ was observed to decrease the blood lipid levels of the AS rabbits; increase the concentration of high-density lipoprotein and apolipoprotein A; decrease blood viscosity, erythrocyte sedimentation rate and hematocrit; elevate the levels of endothelial nitric oxide synthase (eNOS) and Na+/H+ exchanger 1 in vascular tissues and decrease the levels of angiotensin II receptor, type 1 (AT-1) and endothelin-1 (ET-1). In conclusion, XMJ was shown to lower the blood lipid levels of the experimental AS rabbits, improve the abnormal changes in hemorheology, increase the eNOS content in the vascular tissue, decrease the AT-1 and ET-1 levels and increase the endothelium-dependent vasodilation reaction. XMJ therefore has an anti-AS effect.

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“…The XTT method was used to observe the effects of SIPS on the proliferation of CHRF and K562 cells according to previously reported methods 62 . In brief, 100 μl CHRF or K562 cells were seeded into 96-well plates at a concentration of 2 × 10 4 cells/well and cultured in RPMI-1640 medium (contains 15% FBS).…”

Section: Methodsmentioning

confidence: 99%

Sarcodon imbricatus polysaccharides improve mouse hematopoietic function after cyclophosphamide-induced damage via G-CSF mediated JAK2/STAT3 pathway

et al. 2018

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Sarcodon imbricatus, a rare medicinal and edible fungus, has various pharmacological bioactivities. We investigated the effects of S. imbricatus polysaccharides (SIPS) on hematopoietic function and identified the underlying mechanisms using in vitro experiments with CHRF, K562, and bone marrow mononuclear cells (BMMNCs) and in vivo experiments with a mouse model of cyclophosphamide-induced hematopoietic dysfunction. We found that SIPS induced proliferation and differentiation of CHRF and K562 cells and upregulated the expression of hematopoietic-related proteins, including p90 ribosomal S6 kinases (RSK1p90), c-Myc, and ETS transcription factor, in the two cell lines. After 28 days of treatment, SIPS enhanced the bodyweight and thymus indices of the mice, alleviated enlargement of the spleen and liver, and contributed to the recovery of peripheral blood to normal levels. More importantly, the percentages of B lymphocytes and hematopoietic stem cells or hematopoietic progenitor cells were significantly elevated in bone marrow. Based on an antibody chip analysis and enzyme-linked immunosorbent assay, SIPS were found to successfully regulate 12 cytokines to healthy levels in serum and spleen. The cytokines included the following: interleukins 1Ra, 2, 3, 4, 5, and 6, tumor necrosis factor α, interferon−γ, granulocyte colony-stimulating factor (G-CSF) and macrophage colony-stimulating factor (M-CSF), C-C motif chemokine1, and monocyte chemoattractant protein−1. Moreover, SIPS upregulated the phosphorylation levels of janus kinase 2 (JAK2) and the signal transducer and activator of transcription 3 (STAT3) in the spleen, and similar results were validated in CHRF cells, K562 cells, and BMMNCs. The data indicate that SIPS activated the JAK2/STAT3 pathway, possibly by interactions among multiple cytokines, particularly G-CSF. We found that SIPS was remarkably beneficial to the bone marrow hematopoietic system, and we anticipate that it could improve myelosuppression induced by long-term radiotherapy or chemotherapy.

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Protective effect of the ultra-filtration extract from Xin Mai Jia on human aortic smooth muscle cell injury induced by hydrogen peroxide

Cited by 8 publications

References 27 publications

Traditional Chinese medicine xin-mai-jia recouples endothelial nitric oxide synthase to prevent atherosclerosis in vivo

Traditional Chinese medicine xin-mai-jia recouples endothelial nitric oxide synthase to prevent atherosclerosis in vivo

Effects and mechanism of Xin Mai Jia in a rabbit model of atherosclerosis

Sarcodon imbricatus polysaccharides improve mouse hematopoietic function after cyclophosphamide-induced damage via G-CSF mediated JAK2/STAT3 pathway

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