Protective Effect of the Traditional Chinese Patent Medicine Qing‐Xuan Granule against Bleomycin‐Induced Pulmonary Fibrosis in Mice

Abstract

Pulmonary fibrosis (PF) is a chronic obstructive pulmonary disease without effective clinical drug treatment. Qing-Xuan Granule (QX) as a traditional Chinese patent medicine is clinically used to cure children's cough. This study was designed to investigate the effects of QX and possible molecular mechanisms for bleomycin-induced PF. The work used Western blotting and Q-PCR to explore the vitro and vivo mechanisms of QX treatment, while using HPLC-TOF/MS to explore the composition of QX. QX was given daily orally for two weeks after bleomycin intratracheal instillation. The protective effects of QX on lung function, inflammation, growth factors, hydroxyproline content and deposition of extracellular matrix were investigated. QX decreased expression of Col I and α-SMA in lung tissues by down-regulating TGF-β1-Smad2/3 signaling and suppressed epithelialmesenchymal transition and effectively reversed abnormal mRNA levels of MMP-1and TIMP-1 as well as LOXL-2 in lung tissues. HPLC-TOF/MS indicate that six substances could be the main active components, which were reported to protect against experimental lung disease.

Schisandra Inhibit Bleomycin-Induced Idiopathic Pulmonary Fibrosis in Rats via Suppressing M2 Macrophage Polarization

“…In recent years, TCM and their formulations have been shown to exhibit great efficacy in treating lung fibrosis with good prospects for application [20][21][22][23]. Schisandra, the fruit of Schisandra chinensis (Turcz.)…”

From Basic Research to Clinical Practice: Considerations for Treatment Drugs for Silicosis

“…Numerous studies have demonstrated the potential of various TCMs, including Xin Jia Xuan Bai Cheng Qi Decoction extract, Renshen Pingfei Decoction, Yangyin Yiqi Mixture, and Qing-Xuan Granules, to ameliorate the progression of pulmonary fibrosis. The mechanism may involve the suppression of the TGF-β1/Smad signal pathway and EMT in BLM-induced pulmonary fibrosis [114][115][116][117]. Other drugs, such as Feifukang, Modified Kushen Gancao Formula, Jinshui Huanxian Formula, and Yifei Sanjie Formula, target different pathways to alleviate pulmonary fibrosis [118][119][120][121].…”

Traditional Chinese medicine combined with pulmonary drug delivery system and idiopathic pulmonary fibrosis: Rationale and therapeutic potential

“…They use decoction, Chinese patent medicine and TCM injections to perform treatment in patients at different stages of IPF by adjusting the match of the monarch, minister, assistant and guide in TCM. Through the collection and research of a large number of literatures, twenty-seven compound prescriptions of TCM with good curative effect for the treatment of IPF are shown in Table 1 [ [21] , [22] , [23] , [24] , [25] , [26] , [27] , [28] , [29] , [30] , [31] , [32] , [33] , [34] , [35] , [36] , [37] , [38] , [39] , [40] , [41] , [42] , [43] , [44] , [45] , [46] , [47] ].…”