Protective Effect of Tadenan of Bladder Function Secondary to Partial Outlet Obstruction

Levin, Robert M.; Riffaud, J.-P.; Bellamy, F.; Rohrmann, Dorothea; Habib, M. Hadj; Krasnopolsky, Lev; Zhao, Yang; Wein, Alan J.

doi:10.1016/s0022-5347(01)66308-0

Cited by 55 publications

(25 citation statements)

References 16 publications

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“…Both species respond similarly (qualitatively) to bladder outlet partial obstruction with progressive increases in bladder mass resulting from hypertrophy and hyperplasia, as well as increased wall thickness and decreased bladder compliance [for reviews, see 3,4,22,23]. Both species respond with progressive contractile dysfunctions and increased incidence of hyperreflexia and unstable bladder contractions [3,4,23,24].…”

Section: Discussionmentioning

confidence: 99%

Rabbit versus Rat Urinary Bladder: Effects of in vitro Hypoxia

Whitbeck

Barreto²,

Horan³

et al. 1999

Pharmacology

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Purpose: Studies indicate that bladder hypoxia may be an etiological factor for lower urinary tract dysfunction. Rat and rabbit are two species of experimental animals used frequently to study lower urinary tract function and dysfunction. The objective of this study was to compare directly effects of in vitro hypoxia on contractile responses of rat and rabbit urinary bladder to different forms of stimulation. Methods: Sexually mature male New Zealand White rabbits and Sprague-Dawley rats were compared. Each bladder was excised while the animal was anesthetized, and longitudinal bladder strips were cut, then mounted in organ baths. A tension of 2 g was placed on all strips. Effects of 1, 2, 3 and 4 h hypoxia followed by 1 h of reoxygenation on contractile responses of bladder strips to field stimulation (FS), carbachol (100 μmol/l), ATP (1 mmol/l) and KCl (120 mmol/l) were determined. Results: Contractility, per unit tissue mass, of rat bladder strips was significantly greater than that of rabbit bladder strips in response to FS (all frequencies), carbachol, KCl and ATP. Hypoxia (followed by reoxygenation) resulted in time-dependent progressive reduction in contractile responses of bladder strips to all stimuli. Rat bladder was significantly more sensitive to hypoxia than rabbit bladder in response to FS and carbachol. Hypoxia induced similar effects on rat and rabbit bladder responses to ATP and KCl. Conclusion: Rat bladder neurogenic and cholinergic responses are significantly more sensitive to hypoxia than are those of rabbit bladder, which may be due to the rat bladder’s greater contractile force generation and previously reported higher Ca²⁺-ATPase activity.

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Section: Discussionmentioning

confidence: 99%

Rabbit versus Rat Urinary Bladder: Effects of in vitro Hypoxia

Whitbeck

Barreto²,

Horan³

et al. 1999

Pharmacology

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“…BOO is a common problem in ageing men, occurring in > 80% of men in their eighth decade [1]; causes include BPH, urethral stricture, prostate cancer and detrusor sphincter dyssynergia [2]. With time, the obstructed bladder deteriorates due to alterations in its physiology and structure, and it is known that similar changes can also occur in the hypoxic bladder in experimental models [3].…”

Section: Introductionmentioning

confidence: 99%

“…However, reduced bladder wall blood flow during the emptying phase results in further tissue hypoxia [6]. This observation comes from experimental models in animals, where obstruction is artificially induced from days to several weeks [1][2][3][4][5][6][7].…”

Section: Introductionmentioning

confidence: 99%

Immunohistochemical estimation of hypoxia in human obstructed bladder and correlation with clinical variables

Koritsiadis¹,

Stravodimos²,

Koutalellis³

et al. 2008

BJU International

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OBJECTIVETo investigate the tissue distribution of ischaemia in human detrusor in patients with bladder outlet obstruction (BOO) and to correlate the results with clinical variables, as clinical BOO is a common problem in ageing men and ischaemia might be important in detrusor dysfunction.PATIENTS AND METHODSFrom September 2004 to October 2006, 70 patients were recruited, comprising 60 scheduled for surgery to treat benign prostatic hyperplasia (the study group) and 10 as controls. Detrusor tissue was retrieved and stained for hypoxia‐inducible factor (HIF)‐1α, a cellular marker of hypoxia.RESULTSThe mean (sd) total number of cells immunoreactive to HIF‐1α in the study group was 93.3 (48.09), and in the specimens from the control group only few rare cells showed weak immunoreactivity to HIF‐1α (0–2). Positive cells were in different proportions between muscle bundles and submucosa, expressed mainly in stromal cells. The urothelium and detrusor muscle showed no immunoreactivity to HIF‐1α. There was strong immunoreactivity in patients with prolonged BOO (<10 years), declining thereafter, and in those patients with urinary retention.CONCLUSIONSThe urothelium and detrusor seem to be more resistant to hypoxic stress, while stromal cells perceive low oxygen tension. The bladder response to chronic hypoxia through HIF‐1α expression is limited in time and might depend on the functional status of the detrusor.

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“…Thus a role for RyR-CICR in the in vitro activation of the detrusor must be postulated. The potential importance of an internal Ca2' release process in the activation of detrusor has been highlighted by the finding that in obstructed bladders the contractile dysfunction is linked to a downregulation of CICR (Levin et al 1997). In addition, there are data from the neonatal rabbit bladder suggesting that there are alterations in the density of RyR receptors during development.…”

Section: Discussionmentioning

confidence: 99%

Ryanodine receptors in human bladder smooth muscle

Chambers¹,

Neal²,

Gillespie³

1999

Experimental Physiology

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SUMMARY The role of intracellular Ca2+ release in the activation of human bladder smooth muscle is controversial. We have measured the expression of mRNA encoding for the ryanodine receptor (RyR) isoforms (RyR1, RyR2 and RyR3) in isolated human detrusor smooth muscle. mRNA for RyR2 was detected in all samples but no mRNA for RyR1 or RyR3 could be found. Human bladder smooth muscle cells in culture are unresponsive to caffeine, suggesting the absence of a functional RyR system. However, mRNA encoding for RyR2 was detected in these cells. Using saponin‐permeabilized cells, a Ruthenium Red‐sensitive Ca2+‐dependent 45Ca2+ release could be demonstrated from the sarcoplasmic reticulum (SR). These data confirm the functional presence of Ca2+‐induced Ca2+ release (CICR) in cells and suggest that the properties of the RyR2 isoform in human detrusor may change when the cells are maintained in culture. The implications of these observations to detrusor smooth muscle function are discussed.

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Protective Effect of Tadenan of Bladder Function Secondary to Partial Outlet Obstruction

Abstract: These results clearly demonstrate that Tadenan pretreatment protected the bladder from the contractile dysfunctions induced by partial outlet obstruction.

Cited by 55 publications

References 16 publications

Rabbit versus Rat Urinary Bladder: Effects of in vitro Hypoxia

Rabbit versus Rat Urinary Bladder: Effects of in vitro Hypoxia

Immunohistochemical estimation of hypoxia in human obstructed bladder and correlation with clinical variables

Ryanodine receptors in human bladder smooth muscle

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