Protective effect of surface layer proteins isolated from four Lactobacillus strains on hydrogen-peroxide-induced HT-29 cells oxidative stress

Zhao, Binbin; Meng, Jianghong; Zhang, Qiuxiang; Kang, Tingting; Lu, Rongrong

doi:10.1016/j.ijbiomac.2017.03.160

Cited by 29 publications

(18 citation statements)

References 39 publications

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“…In general, SLPs are thought to contribute to interactions with the external environment. Lactobacillus SLPs have been reported to enhance adhesion to the intestinal wall (Hynönen and Palva, 2013), protect against infection (Martínez et al, 2012; Prado Acosta et al, 2016), regulate the immune system (Konstantinov et al, 2008; Beganović et al, 2011; Taverniti et al, 2013), and exhibit an anti-oxidative function (Zhao et al, 2017). Thus, Lactobacillus SLPs have beneficial effects on their host as biogenics.…”

Section: Discussionmentioning

confidence: 99%

S-Layer Protein of Lactobacillus helveticus SBT2171 Promotes Human β-Defensin 2 Expression via TLR2–JNK Signaling

Kobatake

Kabuki

2019

Front. Microbiol.

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Antimicrobial peptides that contribute to innate immunity are among the most important protective measures against infection in many organisms. Several substances are known to regulate the expression of antimicrobial peptides. In this study, we investigated the factors in lactic acid bacteria (LAB) that induce antimicrobial peptide expression in the host. We found that Lactobacillus helveticus SBT2171 (LH2171) induced the expression of human β-defensin (hBD)2 in Caco-2 human colonic epithelial cells. Specifically, surface layer protein (SLP) of LH2171 stimulated hBD2 expression by activating c-Jun N-terminal kinase (JNK) signaling via Toll-like receptor (TLR)2 in Caco-2 cells. SLPs extracted from other lactobacilli similarly increased hBD2 expression, suggesting that this stimulatory effect is common feature of Lactobacillus SLPs. Interestingly, Lactobacillus strains that strongly induced hBD2 expression also potently activated JNK signaling. Thus, upregulation of hBD2 induced by TLR2–JNK signaling contributes to protection of the host against infection.

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Section: Discussionmentioning

confidence: 99%

S-Layer Protein of Lactobacillus helveticus SBT2171 Promotes Human β-Defensin 2 Expression via TLR2–JNK Signaling

Kobatake

Kabuki

2019

Front. Microbiol.

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“…Lactobacillus strains possess an antioxidative capacity which may be related to cell metabolites. A surface layer protein (SLP) isolated from L. acidophilus NCFM played an important role in the antioxidative capacity of HT-29 cells induced by H 2 O 2 (Zhao, Meng, Zhang, Kang, & Lu, 2017). This SLP improved the total antioxidative capacity (T-AOC) and the activities of CAT and SOD and decreased the level of malondialdehyde (Zhao et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

“…A surface layer protein (SLP) isolated from L. acidophilus NCFM played an important role in the antioxidative capacity of HT‐29 cells induced by H 2 O 2 (Zhao, Meng, Zhang, Kang, & Lu, ). This SLP improved the total antioxidative capacity (T‐AOC) and the activities of CAT and SOD and decreased the level of malondialdehyde (Zhao et al., ). In addition, the antioxidative property of exopolysaccharides (EPS) has been assessed in several probiotics such as Lactococcus lactis and Lactobacillus plantarum which have been reported to promote the production of antioxidative enzymes such as SOD (Deepak et al., ).…”

Section: Discussionmentioning

confidence: 99%

Germinated brown rice combined with Lactobacillus acidophilus and Bifidobacterium animalis subsp. lactis inhibits colorectal carcinogenesis in rats

Lin

et al. 2018

Food Science & Nutrition

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Colorectal cancer is a common cancer strongly associated with diet. Certain probiotics and prebiotics possess an inhibitory activity against colorectal cancer, while synbiotics may be more effective in preventing this cancer than either prebiotics or probiotics alone. Germinated brown rice (GBR) is considered as a candidate prebiotics with anticancer potential. However, the effect of GBR combined with probiotics on colorectal cancer is not clear. The present study investigated the preventive effect of combination of GBR and Lactobacillus acidophilus, Bifidobacterium animalis subsp. lactis, or both on colorectal carcinogenesis and the possible mechanism in rats treated with 1,2‐dimethylhydrazine (DMH) and dextran sulfate sodium (DSS). DMH/DSS treatment induced preneoplastic aberrant crypt foci (ACF) and mucin‐depleted foci (MDF), reduced superoxide dismutase (SOD) activity, increased anti‐apoptotic Bcl‐2 expression, and decreased the expression of pro‐apoptotic p53, Bax, and caspase‐3 in the colon. Germinated brown rice alone or combined with probiotics inhibited the formation of MDF in the middle colon, enhanced the colonic expression of p53 and Bax, and increased the ratio of Bax/Bcl‐2. Combined treatment of GBR and probiotics inhibited the formation of ACF‐producing sialomucin (SIM‐ACF) and recovered the activity of SOD in the colon. Combination of GBR and L. acidophilus further increased caspase‐3 expression and decreased Bcl‐2 expression. These findings suggest that GBR combined with L. acidophilus and/or B. animalis subsp. lactis may inhibit colorectal carcinogenesis by enhancing antioxidative capacity and inducing apoptosis. This synbiotics may be a potential functional food or chemopreventive agent for controlling colorectal cancer.

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“…Firstly, LGG is capable of counteracting cancer growth. It has been demonstrated within several in vitro tumor models (including colorectal, ovary, breast, cervical, hepatic and oral squamous) that LGG is able to exert either anti-proliferative effects or anti-metastatic effects [102,103,104,105,106]. This could be mediated through the direct modulation of several host’s proliferation pathways, such as mTOR or WNT [107].…”

Section: Lactobacillus Rhamnosus Gg: a Probiotic Model In Cancermentioning

confidence: 99%

Gut Microbiota and Cancer: From Pathogenesis to Therapy

Vivarelli

Salemi

Candido

et al. 2019

Cancers

432

302

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Cancer is a multifactorial pathology and it represents the second leading cause of death worldwide. In the recent years, numerous studies highlighted the dual role of the gut microbiota in preserving host’s health. Gut resident bacteria are able to produce a number of metabolites and bioproducts necessary to protect host’s and gut’s homeostasis. Conversely, several microbiota subpopulations may expand during pathological dysbiosis and therefore produce high levels of toxins capable, in turn, to trigger both inflammation and tumorigenesis. Importantly, gut microbiota can interact with the host either modulating directly the gut epithelium or the immune system. Numerous gut populating bacteria, called probiotics, have been identified as protective against the genesis of tumors. Given their capability of preserving gut homeostasis, probiotics are currently tested to help to fight dysbiosis in cancer patients subjected to chemotherapy and radiotherapy. Most recently, three independent studies show that specific gut resident species may potentiate the positive outcome of anti-cancer immunotherapy. The highly significant studies, uncovering the tight association between gut microbiota and tumorigenesis, as well as gut microbiota and anti-cancer therapy, are here described. The role of the Lactobacillus rhamnosus GG (LGG), as the most studied probiotic model in cancer, is also reported. Overall, according to the findings here summarized, novel strategies integrating probiotics, such as LGG, with conventional anti-cancer therapies are strongly encouraged.

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Protective effect of surface layer proteins isolated from four Lactobacillus strains on hydrogen-peroxide-induced HT-29 cells oxidative stress

Cited by 29 publications

References 39 publications

S-Layer Protein of Lactobacillus helveticus SBT2171 Promotes Human β-Defensin 2 Expression via TLR2–JNK Signaling

S-Layer Protein of Lactobacillus helveticus SBT2171 Promotes Human β-Defensin 2 Expression via TLR2–JNK Signaling

Germinated brown rice combined with Lactobacillus acidophilus and Bifidobacterium animalis subsp. lactis inhibits colorectal carcinogenesis in rats

Gut Microbiota and Cancer: From Pathogenesis to Therapy

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