Protective Effect of Standardized Extract ofGinkgo bilobaagainst Cisplatin-Induced Nephrotoxicity

Abstract

Cisplatin (CDDP) is a potent antitumor compound widely used with a notably side effect of nephrotoxicity inducing oxidative stress and apoptosis in kidneys. Standardized extract from the leaves of the Ginkgo biloba trees, labeled EGb761 (EGb), has been available on the market for its beneficial effects. The purpose of this study was to investigate the ability of EGb to prevent the nephrotoxic effect of CDDP and the mechanisms involved. Our results showed that EGb treatment restored the levels of creatinine, BUN, MDA, NO, SOD, CAT, GPx, and GSSG/GSH ratio in kidneys after CDDP injection. EGb also exhibited a tendency to decrease the elevated NF-κB translocation and caspase-3 protein levels in CDDP-treated kidneys. We further used a porcine kidney proximal tubular epithelial (LLC-PK1) cell line, finding that EGb accordingly inhibited ROS accumulation and iNOS increase induced by CDDP in vitro. EGb also attenuated IκB degradation and p65 NF-κB phosphorylation triggered by CDDP in LLC-PK1 cells. But EGb failed to influence CDDP-stimulated caspase cascade. These findings suggested that EGb's renoprotective effect might be mediated by not only its well-known antioxidant activity but also the anti-inflammatory activity.

Amelioration of cisplatin-induced nephrotoxicity in rats by triterpenoid saponin of Terminalia arjuna

“…Song et al [13] reported the same findings in renal histology after cisplatin treatment. These histopathological findings were ameliorated by Arjunolic acid administration indicating its renopreventive effect against cisplatin-induced nephrotoxicity.…”

“…In this study, Arjunolic acid, a natural product with antioxidant properties, was used for the first time as a renoprotective agent aiming to reduce or reverse cisplatin-induced nephrotoxicity. The experimental design was taken from previous studies that used natural antioxidants as nephroprotective agents in cisplatininduced renal injury [11,13]. The elevation of serum creatinine and BUN was indicated for cisplatin nephrotoxicity as shown in previous studies [16][17][18].…”

“…Arjunolic acid group Rats received 20 mg/kg Arjunolic acid [12] (Sigma-Aldrich Co., St. Louis, MO, USA) daily by oral gavage for 10 days plus an ip injection of a single dose of cisplatin (7 mg/kg) at day 5 [11,13].…”

Protective Activity of <b><i>Dendropanax Morbifera</i></b> Against Cisplatin-Induced Acute Kidney Injury

“…CP triggers ROS generation in the kidney, and these ROS directly act on multiple cell components, including lipids, proteins, and DNA [30]. Interestingly, previous studies have shown that DP showed anti-oxidant activity [18], and our study demonstrated that DP could reverse CP-induced ROS generation in NRK-52E cells.…”

Renoprotective effects of angiotensin receptor blocker and stem cells in acute kidney injury: Involvement of inflammatory and apoptotic markers

“…29 Our experiment showed a significant elevation of renal caspase-3 and bax expressions in CDDP-treated rats in comparison with normal rats which coincides with previous studies. 23,30,31 To our knowledge, this is the first study to examine the effect of candesartan in CDDP-induced nephrotoxicity. Our results demonstrated that both candesartan and BM-MSCs reduced the levels of serum creatinine and urea in CDDPtreated animals.…”