Protective Effect of Seleno-l-Methionine on Cyclophosphamide-Induced Urinary Bladder Toxicity in Rats

Ayhancı, Adnan; Yaman, Suzan; Şahıntürk, Varol; Uyar, Ruhi; Bayramoğlu, Gökhan; Şentürk, Hakan; Altuner, Yılmaz; Appak, Sıla; Güneş, Sibel

doi:10.1007/s12011-009-8458-y

Cited by 14 publications

(9 citation statements)

References 29 publications

(40 reference statements)

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“…23 In addition, CYP-induced HC causes ROS overproduction, which leads to oxidative stress, peroxidation of lipid and loss of protein activities ultimately. 25,54,55 Moreover, another study indicated that glutathione has similar protective efficiency compared with mesna. It is worth to mention that mesna can only be used in CYP-induced HC because it may cause allergic reactions.…”

Section: Discussionmentioning

confidence: 99%

“…52,53 A few studies described the protective effects of antioxidants, especially sulfhydryl-containing compounds, on CYP-induced HC. 25,54,55 Moreover, another study indicated that glutathione has similar protective efficiency compared with mesna. 56 These findings lead us to further investigate the effects of antioxidant and mesna to CYP-induced HC.…”

Section: Discussionmentioning

confidence: 99%

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Protective effects of lipoic acid and mesna on cyclophosphamide‐induced haemorrhagic cystitis in mice

Song

Liu

et al. 2013

Cell Biochemistry & Function

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The protective roles of lipoic acid (LA)/vitamin C (VC) and mesna on preventing cyclophosphamide (CYP)-induced haemorrhagic cystitis (HC) were investigated. Swiss mice were divided into five groups randomly. HC was induced by a single dose of CYP injection (150-mg kg(-1) bodyweight). Group I was injected with saline (four times in total) throughout as control group. Group II received CYP and three equal doses of saline. Group III received CYP and three doses of mesna, whereas Group IV (or Group V) received CYP, mesna + two doses of VC (or LA). All injections were performed intraperitoneally. After 24 h of cystitis induction, the bladders were collected for all the experiments. Histological characterization showed that CYP injection resulted in severe HC. Reactive oxygen species (ROS) and thiobarbituric acid reactive substances' levels were increased in CYP group. The activities of antioxidant enzymes, e.g. superoxide dismutase, catalase, glutathione S-transferase and glutathione peroxidase, were inhibited significantly in CYP groups, respectively. In addition, activation of c-jun N-terminal kinases (JNK) and p38 mitogen-activated protein kinase (MAPK) may be involved in the mechanism of CYP-induced HC but not extracellular signal regulated kinases (ERK). Significant suppression of p38 phosphorylation on Group V suggests that LA and mesna may have synergistic beneficial effect. In Groups III-V, all the parameters of HC and oxidative stress were inhibited significantly. Taking together, we found that these results illustrated that ROS play an important role on CYP-induced HC and the administration of LA/VC with mesna may have therapeutic potential against CYP-induced bladder HC.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Protective effects of lipoic acid and mesna on cyclophosphamide‐induced haemorrhagic cystitis in mice

Song

Liu

et al. 2013

Cell Biochemistry & Function

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“…ere are numerous reports documenting the role of NO in CP-induced HC [6,9,28]. Additionally, it has been shown that bladder damage may be due not only to the overproduction of NO but also of ROS [29][30][31]. It is well known that reactions of ROS with biomolecules such as lipids can initiate chain reactions and lead to tissue damage [32].…”

Section: Discussionmentioning

confidence: 99%

Effects of Ozone Therapy on Cyclophosphamide-induced Urinary Bladder Toxicity in Rats

Tasdemir¹,

Taşdemir²,

Vardı³

et al. 2013

CIM

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Purpose: is study investigated the e cacy of ozone therapy (OT) in a rat model of cyclophosphamide-induced hemorrhagic cystitis (HC).Methods: Forty Wistar Albino male rats were divided into ve groups: sham, OT, cyclophosphamide (CP), OT+CP and CP+OT. Hemorrhagic cystitis (HC) was induced by intraperitoneal (i.p) administration a single dose of 100 mg/kg CP. OT was performed once daily for three days. e CP+OT group received OT (0.2 mg/kg) i.p 24 h a er CP administration. CP was injected to the OT+CP group the day a er the third course of OT. All animals were killed four days a er CP administration. Bladder injury and oxidative stress parameters were determined from tissue samples.Results: We found small, but non-statistically signi cant biochemical and histological changes in the animals treated with OT alone. CP administration induced cystitis, as manifested by a marked loss of urothelial cells, as well as hemorrhaging and edema in the bladder as determined by histopathological examination. It also caused a signi cant decrease in the endogenous antioxidant compound glutathione (GSH) and elevation of lipid peroxidation, and nitric oxide (NO) and myeloperoxidase (MPO) levels in the rats' urinary bladder tissue. OT was able to ameliorate these changes; however these e ects were prominent in the CP+OT group when compared with the OT+CP group.: For example, the NO level in the CP+OT group was 68% of the OT+CP group (p < 0.05).Conclusion: OT prevented CP-induced urothelial damage by diminishing bladder oxidative stress, in ammation and NO levels. OT may help to ameliorate bladder damage induced by CP in the clinical setting.

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“…CP has been associated with various kinds of urinary tract toxicities [20,21], but kidney toxicity has only rarely been reported. necrosis had developed after a single i.p.…”

Section: Discussionmentioning

confidence: 99%

Seleno l-Methionine Acts on Cyclophosphamide-Induced Kidney Toxicity

Ayhancı

Güneş

Şahıntürk

et al. 2009

Biol Trace Elem Res

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The anticancer drug cyclophosphamide (CP) has nephrotoxic effects besides its urotoxicity, which both in turn limit its clinical utility. The nephrotoxicity of CP is less common compared to its urotoxicity, and not much importance has been given for the study of mechanism of CP-induced nephrotoxicity so far. Overproduction of reactive oxygen species (ROS) during inflammation is one of the reasons of the kidney injury. Selenoproteins play crucial roles in regulating ROS and redox status in nearly all tissues; therefore, in this study, the nephrotoxicity of CP and the possible protective effects of seleno L-methionine (SLM) on rat kidneys were investigated. Forty-two Sprague-Dawley rats were equally divided into six groups of seven rats each. The control group received saline, and other rats were injected with CP (100 mg/kg), SLM (0.5 or 1 mg/kg), or CP+ SLM intraperitoneally. Malondialdehyde (MDA) and glutathione (GSH) levels in kidney homogenates of rats were measured, and kidney tissues were examined under the microscope. CP-treated rats showed a depletion of renal GSH levels (28% of control), while CP+SLM-injected rats had GSH values close to the control group. MDA levels increased 36% of control following CP administration, which were significantly decreased after SLM treatment. Furthermore, these biochemical results were supported by microscopical observations. In conclusion, the present study not only points to the therapeutic potential of SLM in CP-induced kidney toxicity but also indicates a significant role for ROS and their relation to kidney dysfunction.

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Protective Effect of Seleno-l-Methionine on Cyclophosphamide-Induced Urinary Bladder Toxicity in Rats

Cited by 14 publications

References 29 publications

Protective effects of lipoic acid and mesna on cyclophosphamide‐induced haemorrhagic cystitis in mice

Protective effects of lipoic acid and mesna on cyclophosphamide‐induced haemorrhagic cystitis in mice

Effects of Ozone Therapy on Cyclophosphamide-induced Urinary Bladder Toxicity in Rats

Seleno l-Methionine Acts on Cyclophosphamide-Induced Kidney Toxicity

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