Protective effect of Sam-Hwang-Sa-Sim-Tang against hepatic steatosis in mice fed a high-cholesterol diet

Ahn, Tae-Gue; Lee, Jooyoung; Cheon, Se‐Yun; An, Hyo‐Jin; Kook, Yoon‐Bum

doi:10.1186/1472-6882-13-366

Cited by 7 publications

(5 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the liver, we found that animals that were fed with high-cholesterol diets exert conspicuous steatosis and elevated triglycerides. These overall findings agree with previous results showing that SHSST protects against liver steatosis and protects vessels against inflammation arising from the excessive ingestion of cholesterol 37 . The authors of these previous studies believe that hypercholesterolemia should be considered as a risk factor for hepatic fibrosis, in addition to atherosclerosis and coronary artery disease 38 .…”

Section: Discussionsupporting

confidence: 93%

Chronic Moderate Alcohol Intakes Accelerate SR-B1 Mediated Reverse Cholesterol Transport

Diao

Liu

et al. 2016

Sci Rep

View full text Add to dashboard Cite

Cholesterol is essential for all animal life. However, a high level of cholesterol in the body is strongly associated with the progression of various severe diseases. In our study, the potential involvement of alcohol in the regulation of high density lipoprotein (HDL) receptor scavenger receptor class B and type I (SR-B1)-mediated reverse cholesterol transport was investigated. We separated male C57BL/6 mice into four diets: control, alcohol, Control + HC and alcohol + HC. The SR-B1 level and 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate- high- density lipoprotein (DiI-HDL) uptake were also measured in AML12 cells and HL7702 cells treated with alcohol. The control + HC diet led to increased hepatic triglyceride and cholesterol levels while alcohol + HC led no significant change. Compared with that of the control group, the SR-B1 mRNA level was elevated by 27.1% (P < 0.05), 123.8% (P < 0.001) and 343.6% (P < 0.001) in the alcohol, control + HC and alcohol + HC groups, respectively. In AML12 and HL7702 cells, SR-B1 level and DiI-HDL uptake were repressed by SR-B1 siRNA or GW9662. However, these effects were reversed through alcohol treatment. These data suggest that a moderate amount of alcohol plays a novel role in reverse cholesterol transport, mainly mediated by PPARγ and SR-B1.

show abstract

Section: Discussionsupporting

confidence: 93%

Chronic Moderate Alcohol Intakes Accelerate SR-B1 Mediated Reverse Cholesterol Transport

Diao

Liu

et al. 2016

Sci Rep

View full text Add to dashboard Cite

show abstract

“…However, no clinically effective therapeutic agents for the treatment of NAFLD are currently available [38]. Recent studies have reported that many herbal medicines for the treatment of NAFLD tend to use multiple herbs in complex formulations, owing to multitargeting and multifunctional properties, complementary interactions, and synergistic effects demonstrating better or enhanced efficacies over treatment with individual herbs [39][40][41].…”

Section: Discussionmentioning

confidence: 99%

Protective Effect of a Mixture of Astragalus membranaceus and Lithospermum erythrorhizon Extract against Hepatic Steatosis in High Fat Diet‐Induced Nonalcoholic Fatty Liver Disease Mice

Choi

Kim

Park

et al. 2020

Evidence-Based Complementary and Alternative Medicine

View full text Add to dashboard Cite

e present study aimed to evaluate the potential synergistic and protective effects of ALM16, a mixture of Astragalus membranaceus (AM) and Lithospermum erythrorhizon (LE) extract in a ratio of 7 : 3, against hepatic steatosis in high fat diet (HFD)induced nonalcoholic fatty liver disease (NAFLD) mice. Forty-eight mice were randomly divided into eight groups and orally administered daily for 6 weeks with a normal diet (ND) or high fat diet alone (HFD), HFD with AM (HFD + 100 mg/kg AM extract), HFD with LE (HFD + 100 mg/kg LE extract), HFD with ALM16 (HFD + 50, 100, and 200 mg/kg ALM16), or HFD with MT (HFD + 100 mg/kg Milk thistle extract) as a positive control. ALM16 significantly decreased the body and liver weight, serum and hepatic lipid profiles, including triglyceride (TG), total cholesterol (TC), high-density lipoprotein-cholesterol (HDL), and low-density lipoprotein-cholesterol (LDL), and serum glucose levels, compared to the HFD group. Moreover, ALM16 significantly ameliorated the HFD-induced increased hepatic injury markers, including aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), lactate dehydrogenase (LDH), and gamma-glutamyltransferase (GGT)-1. Furthermore, as compared to the mice fed HFD alone, ALM16 increased the levels of phosphorylated AMP-activated protein kinase (p-AMPK) and acetyl-CoA carboxylase (p-ACC), thereby upregulating the expression of carnitine palmitoyltransferase (CPT)-1 and downregulating the expression of sterol regulatory element-binding protein (SREBP)-1c and fatty acid synthase (FAS). ese results demonstrated that ALM16 markedly inhibited HFD-induced hepatic steatosis in NAFLD mice by modulating AMPK and ACC signaling pathways, and may be more effective than the single extracts of AM or LE.

show abstract

“…Mice liver total RNA was extracted using the RNeasy mini kit Primers for mouse LDLR were F: 5'-GCG TAT CTG TGG CTG ACA CC-3' and R: 5'-TGT CCA CAC CAT TCA AAC CC-3', mouse SREBF2 were F: 5'-GGC CTC TCC TTT AAC CCC TT-3' and R: 5'-CAC CAT TTA CCA GCC ACA GG-3', mouse HMGCR were F: 5'-GTG GCA GAA AGA GGG AAA GG-3' and R: 5'-CGC CTT TGT TTT CTG GTT GA-3' [2], and mouse β-actin were F: 5'-TGT CCA CCT TCC AGC AGA TGT-3' and R: 5'-AGC TCA GTA ACA GTC CGC CTA GA-3' [50]. Relative gene expression levels were calculated as previously described [37] using β-actin gene as the internal control and were expressed as the fold-change relative to the HCD-control group.…”

Section: Expression Analysis Of Cholesterol Metabolism-related Genes mentioning

confidence: 99%

Functional Probiotic Characterization and In Vivo Cholesterol-Lowering Activity of Lactobacillus helveticus Isolated from Fermented Cow Milk

Damodharan¹,

Palaniyandi²,

Yang³

et al. 2016

Journal of Microbiology and Biotechnology

View full text Add to dashboard Cite

We characterized the probiotic properties of strains KII13 and KHI1 isolated from fermented cow milk by in vitro and in vivo studies. The strains exhibited tolerance to simulated orogastrointestinal condition, adherence to Caco-2 cells, and antimicrobial activity. Both strains produced bioactive tripeptides, isoleucylprolyl-proline and valyl-prolyl-proline, during fermentation of milk. KII13 showed higher in vitro cholesterol-lowering activity (47%) compared with KHI1 (28%) and ATCC 15009 (22%), and hence, it was selected for in vivo study of cholesterol-lowering activity in atherogenic diet-fed hypercholesterolemic mice. For the study, mice were divided into four groups (., normal diet control group, atherogenic diet control group (HCD), KII13- atherogenic diet group (HCD-KII13), and ATCC 43121-atherogenic diet group (HCD-) as positive control). The serum total cholesterol level was significantly decreased by 8.6% and 7.78% in the HCD-KII13 and HCD- groups ( < 0.05), respectively, compared with the HCD group. Low-density lipoprotein cholesterol levels in both HCD-KII13 and HCD- groups were decreased by 13% and 11%, respectively, compared with the HCD group (both, < 0.05). Analysis of cholesterol metabolism-related gene expression in mice liver showed increased expression of and genes in mice fed with KII13. By comparing all the results, we conclude that KII13 could be used as a potential probiotic strain to produce antihypertensive peptides and reduce serum cholesterol.

show abstract

Protective effect of Sam-Hwang-Sa-Sim-Tang against hepatic steatosis in mice fed a high-cholesterol diet

Cited by 7 publications

References 28 publications

Chronic Moderate Alcohol Intakes Accelerate SR-B1 Mediated Reverse Cholesterol Transport

Chronic Moderate Alcohol Intakes Accelerate SR-B1 Mediated Reverse Cholesterol Transport

Protective Effect of a Mixture of Astragalus membranaceus and Lithospermum erythrorhizon Extract against Hepatic Steatosis in High Fat Diet‐Induced Nonalcoholic Fatty Liver Disease Mice

Functional Probiotic Characterization and In Vivo Cholesterol-Lowering Activity of Lactobacillus helveticus Isolated from Fermented Cow Milk

Contact Info

Product

Resources

About