Protective effect of rutin in comparison to silymarin against induced hepatotoxicity in rats

Abstract

Aim:The aim of this study is to evaluate the hepatoprotective effect of rutin (RTN) in comparison to silymarin (SLM) against acetaminophen (APAP)-induced hepatotoxicity in rats.Materials and Methods:Male Wistar albino rats (n=24) of 3 months age were equally divided into four groups. Group 1 served as normal control. Hepatotoxicity was induced in the remaining three groups with administration of 500 mg/kg po APAP from day 1-3. Groups 2, 3, and 4 were subsequently administered orally with distilled water, 25 mg/kg of SLM, and 20 mg/kg of RTN, respectively, for 11 days. The mean body weights and biomarkers of hepatotoxicity were estimated on day 0, 4 (confirmation of toxicity), and 15 (at the end of treatment). Hematological parameters were evaluated on day 4 and 15. Antioxidant profile and adenosine triphosphatases (ATPases) were assessed at the end of the experiment. Liver tissues were subjected to histopathology and transmission electron microscopy after the sacrifice on day 15.Results:Antioxidant profile, ATPases, and hematological and sero-biochemical parameters were significantly altered, and histopathological changes were noticed in the liver of toxic control group. These changes were reversed in groups 3 and 4 that were administered with SLM and RTN, respectively.Conclusion:The results of the present investigation enunciated that SLM has potent hepatoprotective activity though the RTN was found superior in restoring the pathological alterations in paracetamol-induced hepatotoxicity in Wistar albino rats.

Vitamin E is a potent regulator of inflammatory cytokines released during hepatic toxicity in rats

“…It is also suggested that paracetamol inhibits erythropoietin release from the kidney (Samuel et al, 2015). These effects of paracetamol on RBCs, Hb concentration and hematocrit are matched with Oyedeji et al, (2013); Reddy et al, (2017);Sini et al, (2017).…”

Mechanisms of acetaminophen-induced liver injury and its implications for therapeutic interventions

“…for 11 days Wistar rats, 500 mg/kg p.o. for 3 days ↑SOD, CAT, GPx and GSH;↓MDA [98] Astaxanthin 30–60 mg/kg i.g. for 14 days C57BL/6, 300 mg/kg i.p.…”

Efeito Da Rutina No Tratamento Da Cirrose Hepática Induzida Por Tioacetamida: Estudo Experimental Em Ratos / Effect of Rutin in the Treatment of Thioacetamide-Induced Liver Cirrhosis: Experimental Study in Rats

“…(SIKDER et al 2014). Reddy et al (2017), visando comparar os efeitos protetores da rutina -20mg/kg administrado por via oral, durante 11 dias -aos da silimarina sobre a toxicidade hepática induzida por acetaminofeno (APAP), descreveu a presença de dilatação sinusoidal e infiltração periportal, necrose coagulativa e infiltrado mononuclear, após análise histológica do fígado de ratos que receberam apenas a APAP -500mg/kg, via gavagem. O grupo que, além da hepatotoxicidade induzida por APAP, recebeu tratamento com a rutina apresentou apenas alterações degenerativas moderadas, leve congestão e infiltrado mononuclear.…”

“…Sugere-se que, a rutina administrada posteriormente ao desenvolvimento da cirrose hepática possa ser mais efetiva na atenuação da resposta inflamatória. (REDDY et al, 2017). Czechowska et al (2015) induziram fibrose hepática em ratos Wistar através de injeções intraperitoneais de TAA, na dose de 300mg/kg, durante 12 semanas, para avaliar o efeito hepatoprotetor da melatonina administrada também por via intraperitoneal, na dose de 10mg/kg pelas mesmas 12 semanas.…”