Protective effect of Rotula aquatica Lour against gentamicin induced oxidative stress and nephrotoxicity in Wistar rats

Vysakh, A.; Abhilash, S; Jayesh, Kuriakose; Midhun, Sebastian Jose; Mathew, Jyothis; Latha, M.S.

doi:10.1016/j.biopha.2018.07.066

Cited by 41 publications

(16 citation statements)

References 40 publications

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“…The production of reactive oxygen species (ROS) and inflammation play crucial roles in the pathogenesis of GMinduced AKI (Vysakh et al 2018). A state of imbalance between excessive oxidant (free) radicals and insufficient degradation of these radicals by antioxidant systems results in several metabolic dysregulations causing kidney damage (Daenen et al 2019).…”

Section: Discussionmentioning

confidence: 99%

Protective Effects Of A Lipid Transfer Protein Isolated from Morinda citrifolia Seeds in Gentamicin-Induced Nephrotoxicity in Rats

Silveira

Chaves-Filho

Macêdo

et al. 2020

Rev. Bras. Farmacogn.

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Gentamicin is an aminoglycoside antibiotic used in the treatment of gram-negative bacterial infections. However, its prolonged use is limited due to a high risk of nephrotoxicity. This study aimed to investigate the protective effects of a lipid transfer protein isolated from Morinda citrifolia L., Rubiaceae, seeds against gentamicin-induced nephrotoxicity in rats, and in a proximal tubular epithelial cell line from Rhesus monkey (LLC-MK2). Treatment with 6.25-25 μg/ml of the protein protected cells against cytotoxicity induced by 2 mM gentamicin. In the in vivo study, protein treatment (8 mg/kg, orally administered daily for 7 days 2 h after intraperitoneal injection of 100 mg/kg gentamicin) improved the levels of creatinine, urea, and uric acid. Additionally, it alleviated oxidative stress and inflammation induced by gentamicin, including a reduction in myeloperoxidase activity and cytokine modulation. Data were corroborated with histological analysis showing attenuated alterations by protein treatment. These findings suggest that this protein protects against gentamicin-induced nephrotoxicity. In addition, this natural product may be as an adjunctive tool to reduce kidney toxicity and improve the clinical usage profile of gentamicin.

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Section: Discussionmentioning

confidence: 99%

Protective Effects Of A Lipid Transfer Protein Isolated from Morinda citrifolia Seeds in Gentamicin-Induced Nephrotoxicity in Rats

Silveira

Chaves-Filho

Macêdo

et al. 2020

Rev. Bras. Farmacogn.

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“…Increased ROS production is an important mechanism for GM-induced nephrotoxicity. Excessive levels of ROS can damage DNA, lipids and proteins, eventually leading to apoptosis 25 . SDAP has been shown to interact with ROS, thereby preventing ROS-induced tissue and cell damage.…”

Section: Discussionmentioning

confidence: 99%

Purified Sika deer antler protein attenuates GM-induced nephrotoxicity by activating Nrf2 pathway and inhibiting NF-κB pathway

Wang

Wang³

et al. 2020

Sci Rep

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Although gentamicin is widely used as an antibiotic in clinical practice, it also has some side-effects, such as acute kidney injury, which is a common condition caused by the abuse of gentamicin. Sika deer antler protein (SDAPR) can antagonize drug-induced AKI. Since SDAPR is recognized as an effective part of velvet antler, its components were further separated. Two components named SDAP1 and SDAP2 were obtained. The protective effects of SDAPR, SDAP1 and SDAP2 on GM-induced cytotoxicity to HEK293 and its potential mechanisms were studied. MTT and xCELLigence Real-Time cell analysis showed that SDAPR, SDAP1 and SDAP2 could protect HEK293 cells from GM toxicity. Similarly, SDAPR, SDAP1 and SDAP2 can reduce ROS level, reduce oxidative stress and improve inflammation Further studies have shown that SDAPR, SDAP1 and SDAP2 upregulate the Nrf2/HO-1 pathway by increasing the expression of Nrf2 and HO-1, and down-regulate the NF-κB pathway by reducing the protein expression of NF-κB. Annexin V/PI flow cytometry and Hoechst 33258 staining showed that SDAPR, SDAP1 and SDAP2 inhibited GM-induced apoptosis in HEK293 cells. Western blot analysis showed SDAPR, SDAP1 and SDAP2 decreased expression level of Bax and Cleaved-caspase-3, and increased the expression level of Bcl-2. In addition, we examined the feasibility of SDAP1 and SDAP1 to avoid kidney injury in a GM mouse model. In conclusion, SDAPR, SDAP1 and SDAP2 can be used to prevent GM-induced HEK293 cytotoxicity, probably because they have strong anti-oxidative stress, anti-inflammatory and anti-apoptotic effects. And SDAP1 and SDAP2 can inhibit GM-induced acute kidney injury in mice.

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“…This kind of nephritic toxicity can be characterized based on the elevated levels of serum creatinine and urea nitrogen, and reduced glomerular filtration rate (GFR) and urine osmolality (Gowda et al, 2010). Even though, past from decades number of extensive preclinical and clinical studies were continuously going on but the exact mechanism at molecular level of GM-induced nephrotoxicity is not completely explained and currently, the available therapies for established nephrotoxicity has not specified the predictable achievement (Cui et al, 2015;Vysakh et al, 2018). Strong evidence proposes that level of oxidative stress, various inflammatory cytokines causing inflammation and apoptosis are involved in the pathogenesis of GM-induced nephrotoxicity.…”

Section: Introductionmentioning

confidence: 99%

Combinational effect of curcumin and metformin against gentamicin‐induced nephrotoxicity: Involvement of antioxidative, anti‐inflammatory and antiapoptotic pathway

et al. 2019

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Gentamicin (GM) is an antibiotic related to aminoglycoside group that is used in treating Gram‐negative bacterial infections. However, treatment with gentamicin is considered to be limited as it induces an oxidative stress‐mediated apoptosis in kidney which causes a nephrotoxicity. Metformin is a well‐known biguanide that is used for treating diabetes mellitus, especially type 2. Supplement with plant metabolites or natural antioxidants produce a protective activity against many types of diseases in vivo. Curcumin is a main medicinal constituent of Curcuma longa, has reported for number of biological effects, such as antioxidant, anti‐inflammatory, and antitumor. The study aims at evaluating the metformin and curcumin alone or in combination on nephrotoxicity induced by GM. The outcome of the study shows that both metformin and curcumin, when used unaided, were effectively decreasing GM‐induced nephrotoxicity. The two drugs combination was showed synergistic effect in ameliorating a GM‐induced kidney injury, as supported by expressively improved renal dysfunction. Metformin and curcumin showed strong protection against oxidative stress in GM treated animals through decreasing the activities and expression of various antioxidative enzymes. Moreover, combination of two drugs showed an anti‐inflammatory response through reducing a level of pro‐inflammatory cytokines including tumor necrosis factor‐alpha, interleukin 1‐beta, and interleukin 6 in GM intoxicated group of animals. Furthermore, GM agitated apoptosis was affectedly diminished by the combinational treatment of metformin and curcumin via down‐regulating activity of cleaved Caspase‐3 and pro‐apoptotic factor Bax, whereas increasing anti‐apoptotic factor Bcl‐2 signaling pathways. The above results suggested that combinational treatment of metformin and curcumin might be have a synergizing effect and substantial potential against nephrotoxicity induced by GM. Practical applications Curcumin and metformin combination exhibited substantial synergistic effect against GM‐induced nephrotoxicity through reducing oxidative stress, inflammation, as well as apoptosis in kidney cells. Therefore, the method of combination of curcumin and metformin might be functional to treat or inhibit GM prompted nephrotoxicity in future.

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Protective effect of Rotula aquatica Lour against gentamicin induced oxidative stress and nephrotoxicity in Wistar rats

Cited by 41 publications

References 40 publications

Protective Effects Of A Lipid Transfer Protein Isolated from Morinda citrifolia Seeds in Gentamicin-Induced Nephrotoxicity in Rats

Protective Effects Of A Lipid Transfer Protein Isolated from Morinda citrifolia Seeds in Gentamicin-Induced Nephrotoxicity in Rats

Purified Sika deer antler protein attenuates GM-induced nephrotoxicity by activating Nrf2 pathway and inhibiting NF-κB pathway

Combinational effect of curcumin and metformin against gentamicin‐induced nephrotoxicity: Involvement of antioxidative, anti‐inflammatory and antiapoptotic pathway

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