Protective effect of resveratrol on estrogen deficiency-induced osteoporosis though attenuating NADPH oxidase 4/nuclear factor kappa B pathway by increasing miR-92b-3p expression

Zhang, Ye; Liu, Ming‐Wei; He, Yun; Deng, Ning; Chen, Yan; Huang, Jiecong; Xie, Wei

doi:10.1177/2058738420941762

Cited by 26 publications

(49 citation statements)

References 48 publications

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“…Recent studies have demonstrated that the expression levels of various miRNAs are significantly altered, which may be involved in the pathophysiological process of I/R injury, including miR-497, miR-1, and miR-181, which are related to neuroprotection [ 22 , 23 ]. Recently, a study has shown that miR-92b-3p, may be involved in the occurrence of oxidative stress injury during cerebral I/R induced by NOX4 overexpression, which eventually leads to the accumulation of oxygen free radicals and the death of neuronal cells from oxidative stress injury [ 24 ]. This study showed that miR-92b-3p was significantly decreased in a cerebral I/R injury rat model.…”

Section: Introductionmentioning

confidence: 99%

miR-92b-3p Exerts Neuroprotective Effects on Ischemia/Reperfusion-Induced Cerebral Injury via Targeting NOX4 in a Rat Model

Huang

Tang

et al. 2022

Oxidative Medicine and Cellular Longevity

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The necessity to increase the efficiency of organ preservation has pushed researchers to consider the mechanisms to minimize cerebral ischemia/reperfusion (I/R) injury. Hence, we evaluated the role of the miR-92b-3p/NOX4 pathway in cerebral I/R injury. A cerebral I/R injury model was established by blocking the left middle cerebral artery for 2 h and reperfusion for 24 h, and a hypoxia/reoxygenation (H/R) model was established. Thereafter, cerebral I/R increased obvious neurobiological function and brain injury (such as cerebral infarction, apoptosis, and cell morphology changes). In addition, we noted a significant decrease in the expression of miR-92b-3p, as well as increases in apoptosis and oxidative stress and an increase in NOX4. Furthermore, overexpression of miR-92b-3p blocked the inhibitory effect of miR-92b-3p on the expression of NOX4 and the accumulation of oxygen-free radicals. Bioinformatics analysis found that NOX4 may be the target gene regulated by miR-92b-3p. In conclusion, the involvement of the miR-92b-3p/NOX4 pathway ameliorated cerebral I/R injury through the prevention of apoptosis and oxidative stress. The miR-92b-3p/NOX4 pathway could be considered a potential therapeutic target to alleviate cerebral I/R injury.

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Section: Introductionmentioning

confidence: 99%

miR-92b-3p Exerts Neuroprotective Effects on Ischemia/Reperfusion-Induced Cerebral Injury via Targeting NOX4 in a Rat Model

Huang

Tang

et al. 2022

Oxidative Medicine and Cellular Longevity

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“…The role of psychological stress and excessive cortisol secretion in oral disease such as OLP (Gaur et al, 2018) has been reported in several studies. Low levels of estrogen in post‐menopausal women lead to induction of the cellular immune system (Gameiro et al, 2010), increased activity of T‐Cytotoxic cells increases the production of inflammatory cytokines by activation of nuclear factor kappa B (NF‐κB) (Zhang et al, 2020), induce oxidative stress (Bourgonje et al, 2020), and decrease the production of anti‐inflammatory cytokines (Humberto et al, 2018; Upadhyay et al, 2010). The immunomodulatory, anti‐inflammatory, induction of cell proliferation, and cell protection effects of HSP70 have been reported in some studies.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of exposure to environmental stressors on heat‐shock protein 70 expression in normal oral keratinocyte cells

Sheykhbahaei

Koopaie

Ansari

2021

Clinical & Exp Dental Res

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Objectives This study aimed to investigate the effect of cortisol, estrogen, and nicotine on heat shock protein 70 (HSP70) expressions at the level of normal oral mucosa keratinocyte cells. Methods In this in vitro study, keratinocytes were derived from rat oral cavity and cultured. Stressors were applied, including three groups, group 1: estrogen to simulate the postmenopausal state; group 2: cortisol to simulate psychological stress situation; group 3: nicotine to simulate smoking state. To determine the exact nature of keratinocyte cells, two surface markers, cytokeratin 18 and cytokeratin 14 were examined using the flow cytometry method. Then, the immunocytochemistry technique with three repetitions in each group was used to evaluate the HSP70 expression before and after applying the stressor. Results HSP70 expressions in the three stressor groups (estrogen, cortisol, and nicotine) were significantly lower than in the control group (p = 0.0001). The HSPs expression difference between cortisol and nicotine was statistically significant (p = 0.0001). Based on the results of MTT analysis, the mean cell viability of oral mucosal keratinocytes in all three intervention groups decreased compared to the control group. In the cortisol and nicotine groups, cell death was significantly higher than in the control group. In the estrogen group, cell death was significantly lower than in the nicotine group (p > 0.05). Conclusions The specific concentrations of cortisol, estrogen, and nicotine as stressors can effectively reduce the expression of HSP70 in normal oral mucosal keratinocytes. These phenomena can be effective in cell viability and the development of oral lichen planus.

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“…Penzkofer, D., et al proved the deletion of miR-92a will induce skeletal defects [22]. Increasing the expression level of miR-92b will suppress the Cathepsin K expression and Nox4/NF-κB signaling pathway, which can alleviate the progression of osteoporosis [23]. In the one hand the upregulated miR-92b accelerates osteogenesis of BMSCs.…”

Section: Discussionmentioning

confidence: 99%

Integrated Analysis of Hub Genes and miRNAs in Osteoporosis

Huang

Zhao

et al. 2021

Preprint

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Background and objective: Osteoporosis (OP) is a systemic disease of bone metabolism, characterized by decreasing bone mass, increasing bone microstructure damages and fracture risk. It affects the quality of life of nearly 200 million people worldwide and is a major burden on the public health systems. We want to identify hub genes and miRNAs by the miRNA-mRNA interaction network in osteoporosis disease so that further understand the pathogenesis of this disease.Materials and methods: The differentially expressed miRNAs (DEMis) and mRNAs (DEMs) were selected using data of OP patients downloaded from the GEO database (GSE93883, GSE74209 and GSE35959). Gene Ontology (GO) pathway analysis and transcription factor enrichment analysis were used for selecting DEMis, and the target mRNAs of DEMis were filtered by using FunRich. Cytoscape software was used to visualize the network between miRNAs and mRNAs and calculate the hub genes. GO and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were used to analyze the mRNAs in the regulatory network.Results: A total of 17 DEMis and 655 DEMs were selected, from which we reconstructed the miRNA-mRNA network consisting of 6 miRNAs and 37 mRNAs. The top 10 nodes, hsa-let-7a-5p, hsa-miR-92a-3p, hsa-miR-92b-3p, hsa-miR-223-3p, hsa-miR-320c, SLC2A3(Solute Carrier Family 2 Member 3), LBX1(ladybird homeobox 1), HCN2(hyperpolarization-activated cyclic nucleotide-gated ion channel 2), DAB2IP(DAB2 Interacting Protein) and CIC(capicua transcriptional repressor), were identified as important regulators.Conclusions: The study uncovered several important hub genes and miRNAs involved in the pathogenesis of OP, among which, the hsa-let-7a-5p, hsa-miR-92a-3p and hsa-miR-92b-3p may play an important role in osteoporosis, which can help us provide potential therapeutic targets of OP.

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Protective effect of resveratrol on estrogen deficiency-induced osteoporosis though attenuating NADPH oxidase 4/nuclear factor kappa B pathway by increasing miR-92b-3p expression

Cited by 26 publications

References 48 publications

miR-92b-3p Exerts Neuroprotective Effects on Ischemia/Reperfusion-Induced Cerebral Injury via Targeting NOX4 in a Rat Model

miR-92b-3p Exerts Neuroprotective Effects on Ischemia/Reperfusion-Induced Cerebral Injury via Targeting NOX4 in a Rat Model

Evaluation of exposure to environmental stressors on heat‐shock protein 70 expression in normal oral keratinocyte cells

Integrated Analysis of Hub Genes and miRNAs in Osteoporosis

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