Protective effect of resveratrol against naphthalene-induced oxidative stress in mice

Şehirli, Özer; Tozan, Ayfer; Omurtag, Gülden Zehra; Çetınel, Şule; Contuk, Gazi; Gedik, Nursal; Şener, Göksel

doi:10.1016/j.ecoenv.2007.08.023

Cited by 91 publications

(64 citation statements)

References 35 publications

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“…The antioxidant enzyme system in cells is the main indicator of cellular antioxidant levels, it can scavenge ROS and maintain the balance between oxidants and antioxidants. MDA is an important indicator, which reflects the extent of the attack by free radical and its content can reflect the degree of lipid peroxidation in the cell (Sehirli et al 2008). Fraile-Bermudez et al (2015) found that the GPx antioxidant emzyme activity was positively correlated with CAT activity and that the antioxidant enzymes SOD and GPx negatively correlated with MDA (Mladenov et al 2015).…”

Section: Discussionmentioning

confidence: 99%

Vitamin A affects the expression of antioxidant genes in bovine mammary epithelial cells with oxidative stress induced by diethylene triamine-nitric oxide

Shi¹,

Yan²,

Jin³

et al. 2016

CZECH J ANIM SCI

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ABSTRACT:The considerable increase in oxygen requirements due to the high metabolic rate of the bovine mammary epithelial cells (BMEC) during lactation results in an augmented production of reactive nitrogen species (RNS), such as nitric oxide (NO), which may expose cows to increased oxidative stress. Vitamin A (VA) has been shown in several studies to enhance the antioxidant defence system against oxidative stress, but whether the reason is related to a reduced NO production remains unclear. Diethylene triamine-nitric oxide polymer (NOp) is a type of NO-generating compound, which is safe, efficacious, and releases NO over a long period. The current study was conducted to investigate the effect of VA on the antioxidant function in BMEC and the underlying mechanism by discussing the protection of VA on NO-induced oxidative stress of BMEC. The experiment was conducted using a single-factor completely randomized arrangement. Primary BMEC were isolated from the mammary glands of Holstein dairy cows. The third generation cells were randomly divided into four equal groups with six replicates each. Each group received different combinations of VA and NOp treatment as follows: controls (without VA and NOp), NOp treatment alone, VA treatment alone, and VA and NOp treatment together. The lysates were collected to evaluate the activities of glutathione peroxidase (GPx) and thioredoxin reductase (TrxR) and the contents of reactive oxygen species (ROS) and malondialdehyde (MDA), and the cell-free supernatants were collected to analyze selenoprotein P (SelP) content, inducible nitric oxide synthase (iNOS) activities and nitric oxide (NO), interleukin-1 (IL-1), interleukin-6 (IL-6), tumour necrosis factor-α (TNF-α) contents. The results suggested that compared to the control, the cell proliferation, the activity of the antioxidants GPx and TrxR, the content of SelP and the antioxidant gene expressions of GPx1, GPx4, and TrxR1 were significantly decreased (P < 0.05), and the contents of ROS and MDA, the activity of iNOS, the contents of NO and IL-1, IL-6, TNF-α, and their mRNA expressions were increased dramatically in the NOp treatment alone group (P < 0.05), but the opposite changes were observed in the VA treatment alone group. Compared to the NOp treatment alone, the VA and NOp treatment together significantly improved cell proliferation, the activities of the antioxidants GPx and TrxR, and the gene expressions of GPx1 and TrxR1, and dramatically decreased the contents of ROS and MDA, the activity of iNOS, the contents of NO and IL-1, IL-6, TNF-α and their mRNA expression levels (P < 0.05). The present research suggests that VA can improve the antioxidant function of BMEC and protect the cells from experiencing the NOp-induced oxidative stress by regulating antioxidant gene expression. The probable mechanism is that VA can reduce the activity of iNOS and its mRNA expression by down-regulating of the expression of IL-1, IL-6, and TNF-α to reduce NO production. However, the exact mechanism warrants future exploration. Keyword...

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Section: Discussionmentioning

confidence: 99%

Vitamin A affects the expression of antioxidant genes in bovine mammary epithelial cells with oxidative stress induced by diethylene triamine-nitric oxide

Shi¹,

Yan²,

Jin³

et al. 2016

CZECH J ANIM SCI

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“…The peroxidase lipid generated MDA by the decomposition of the peroxidase; MDA is widely used as an index of lipid peroxidation (Sehirli et al 2008). MDA polymerization and crosslinking to form lipofuscin with proteins, peptides, or lipids caused changes in membrane structure and function and increased cell damage.…”

Section: Discussionmentioning

confidence: 99%

Methionine protects against hyperthermia-induced cell injury in cultured bovine mammary epithelial cells

Han

Yang

2015

Cell Stress and Chaperones

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The aim of this study was to investigate the effects of methionine on cell proliferation, antioxidant activity, apoptosis, the expression levels of related genes (HSF-1, HSP70, Bax and Bcl-2) and the expression levels of protein (HSP70) in mammary epithelial cells, after heat treatment. Methionine (60 mg/L) increased the viability and attenuated morphological damage in hyperthermia-treated bovine mammary epithelial cells (BMECs). Additionally, methionine significantly reduced lactate dehydrogenase leakage, malondialdehyde formation, nitric oxide, and nitric oxide synthase activity. Superoxide dismutase, catalase, and glutathione peroxidase enzymatic activity was increased significantly in the presence of methionine. Bovine mammary epithelial cells also exhibited a certain amount of HSP70 reserve after methionine pretreatment for 24 h, and the expression level of the HSP70 gene and protein further increased with incubation at 42°C for 30 min. Compared to the control, the expression of HSF-1 mRNA increased, and there was a significantly reduced expression of Bax/Bcl-2 mRNA and a reduced activity of caspase-3 against heat stress. Methionine also increased survival and decreased early apoptosis of hyperthermia-treated BMECs. Thus, methionine has cytoprotective effects on hyperthermia-induced damage in BMECs.

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“…Along with saline or nicotine injections that were continued for 28 days, either resveratrol (99% purity; Mikrogen Pharmaceuticals, Turkey; RVT; 10 mg/kg/day) or saline was administered daily by intragastric gavage. The dose and duration of nicotine and resveratrol treatments are based on our previous studies (6)(7)(8)28). Each experimental subgroup consisted of 16 rats.…”

Section: Experimental Groupsmentioning

confidence: 99%

“…Several studies have documented that smoking increases the risk of developing urinary tract cancers (20)(21)(22) and the risk for end-stage renal failure in patients with renal disease (23). Recently, several studies have shown that resveratrol exerts protective effects on acute nephrotoxicity in rats (24)(25)(26)(27) and mice (28) by suppressing the inflammatory processes and by inhibiting lipid peroxidation. Moreover, resveratrol has a positive effect on male reproductive function by triggering penile erection, as well as enhancing blood testosterone levels, testicular sperm counts, and epididymal sperm motility (29).…”

Section: Introductionmentioning

confidence: 99%

Resveratrol supplementation protects against chronic nicotine-induced oxidative damage and organ dysfunction in the rat urogenital system

Toklu¹

2010

mpj

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Protective effect of resveratrol against naphthalene-induced oxidative stress in mice

Cited by 91 publications

References 35 publications

Vitamin A affects the expression of antioxidant genes in bovine mammary epithelial cells with oxidative stress induced by diethylene triamine-nitric oxide

Vitamin A affects the expression of antioxidant genes in bovine mammary epithelial cells with oxidative stress induced by diethylene triamine-nitric oxide

Methionine protects against hyperthermia-induced cell injury in cultured bovine mammary epithelial cells

Resveratrol supplementation protects against chronic nicotine-induced oxidative damage and organ dysfunction in the rat urogenital system

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