Protective Effect of Repeatedly Preadministered Brazilian Propolis Ethanol Extract against Stress‐Induced Gastric Mucosal Lesions in Rats

Abstract

The present study was conducted to clarify the protective effect of Brazilian propolis ethanol extract (BPEE) against stress-induced gastric mucosal lesions in rats. The protective effect of BPEE against gastric mucosal lesions in male Wistar rats exposed to water-immersion restraint stress (WIRS) for 6 h was compared between its repeated preadministration (50 mg/kg/day, 7 days) and its single preadministration (50 mg/kg). The repeated BPEE preadministration attenuated WIRS-induced gastric mucosal lesions and gastric mucosal oxidative stress more largely than the single BPEE preadministration. In addition, the repeated BPEE preadministration attenuated neutrophil infiltration in the gastric mucosa of rats exposed to WIRS. The protective effect of the repeated preadministration of BPEE against WIRS-induced gastric mucosal lesions was similar to that of a single preadministration of vitamin E (250 mg/kg) in terms of the extent and manner of protection. From these findings, it is concluded that BPEE preadministered in a repeated manner protects against gastric mucosal lesions in rats exposed to WIRS more effectively than BPEE preadministered in a single manner possibly through its antioxidant and anti-inflammatory actions.

Protective Effect of Brazilian Propolis Against Hepatic Oxidative Damage in Rats with Water‐immersion Restraint Stress

“…Thus, the protective effect of BPEE against WIRS‐induced liver cell damage was found to be diminished at a high dose. In previous reports (Ohta et al , ; Nakamura et al , ) we have confirmed that BPEE (10 or 50 mg/kg) and VE (250 mg/kg) preadministered to rats with 6 h of WIRS have no effect on stress responses, whereas BPEE (100 mg/kg) preadministered to the stressed rats enhances stress responses. In addition, we have suggested that orally administered BPEE could exert a less protective effect against gastric mucosal lesions in rats with 6 h of WIRS at a dose of 100 mg/kg than at a dose of 50 mg/kg by enhancing the sensitivity of stress responses (Nakamura et al , ).…”

“…In previous reports (Ohta et al , ; Nakamura et al , ) we have confirmed that BPEE (10 or 50 mg/kg) and VE (250 mg/kg) preadministered to rats with 6 h of WIRS have no effect on stress responses, whereas BPEE (100 mg/kg) preadministered to the stressed rats enhances stress responses. In addition, we have suggested that orally administered BPEE could exert a less protective effect against gastric mucosal lesions in rats with 6 h of WIRS at a dose of 100 mg/kg than at a dose of 50 mg/kg by enhancing the sensitivity of stress responses (Nakamura et al , ). Therefore, it is suggested that BPEE preadministered at a dose of 100 mg/kg could diminish the protective effect of BPEE against liver cell damage in rats with 6 h of WIRS by enhancing the sensitivity of stress responses.…”

“…Seven-weekold rats were fasted for 24 h prior to experiments, but were allowed free access to water. Water-immersion restraint stress was exposed to fasted rats as follows: fasted rats were restrained in wire cages and immersed up to the depth of the xiphoid process in a 23 C water bath for 6 h, as described in our previous reports (Ohta et al, 2007(Ohta et al, , 2009Kaida et al, 2010;Nakamura et al, 2011) Administrations of BPEE and VE. Brazilian propolis ethanol extract was diluted with 5% Tween 80 solution.…”

Inhibitory effects of tropical almond leaf extract on xanthine oxidase, pancreatic lipase, and angiotensin 1-converting enzyme, in vitro

“…The method earlier reported by Nakamura, Ohta, Ikeno, Ohashi, and Ikeno () was followed to prepare the rat liver homogenate that was used as the source of XO in this study. Briefly, 3 Wistar rats, weighing 200–220 g, were mildly anesthetized with ether, and sacrificed by decapitation.…”

The Possible Role of Propolis in Ameliorating Paclitaxel-Induced Peripheral Neuropathy in Sciatic Nerve of Adult Male Albino Rats

“…In addition, it acts as an antioxidant drug by decreasing the extent of lipid peroxidation (LPO) and increasing the activities of enzymatic antioxidants [52] . Moreover, it is a powerful ROS scavenger [53] as it enhances the levels of reduced glutathione (GSH) suggesting GSHdependent detoxification of free radicals [54] . Propolis also inhibits the migration of neutrophil thus reduces the inflammatory response [55] .…”