Protective effect of quercetin, EGCG, catechin and betaine against oxidative stress induced by ethanol in vitro

Oliva, Joan; Bardag‐Gorce, Fawzia; Tillman, Brittany; French, Samuel W.

doi:10.1016/j.yexmp.2011.02.006

Cited by 64 publications

(49 citation statements)

References 42 publications

(40 reference statements)

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“…However, QE markedly decreased the levels of ROS, TBARS and the CYP2E1 expression in livers of mice (Figs. 3 and 4), which may be due to the powerful antioxidant and free radical scavenging activities [26,35]. Superoxide dismutases (SOD) and catalase (CAT) are important antioxidant enzymes.…”

Section: Discussionmentioning

confidence: 99%

Quercetin protects mouse liver against CCl4-induced inflammation by the TLR2/4 and MAPK/NF-κB pathway

Xie

et al. 2015

International Immunopharmacology

152

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Section: Discussionmentioning

confidence: 99%

Quercetin protects mouse liver against CCl4-induced inflammation by the TLR2/4 and MAPK/NF-κB pathway

Xie

et al. 2015

International Immunopharmacology

152

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“…They have been reported to prevent various diseases associated with oxidative stress, such as cancer, cardiovascular diseases and neurodegenerative diseases (Russo et al ., 2012). Quercetin and catechin are known to have high free radical scavenging activity (Ross and Kasum, 2002), and they exhibit in vivo and in vitro hepatoprotective effects from ethanol-induced oxidative stress (Molina et al ., 2003; Liu et al ., 2010; Oliva et al ., 2011) (Fig. 1).…”

Section: Introductionmentioning

confidence: 99%

Potential in vitro Protective Effect of Quercetin, Catechin, Caffeic Acid and Phytic Acid against Ethanol-Induced Oxidative Stress in SK-Hep-1 Cells

Lee¹,

Kang²,

Yun³

et al. 2012

Biomolecules and Therapeutics

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Phytochemicals have been known to exhibit potent antioxidant activity. This study examined cytoprotective effects of phytochemicals including quercetin, catechin, caffeic acid, and phytic acid against oxidative damage in SK-Hep-1 cells induced by the oxidative and non-oxidative metabolism of ethanol. Exposure of the cells to excess ethanol resulted in a significant increase in cytotoxicity, reactive oxygen species (ROS) production, lipid hydroperoxide (LPO), and antioxidant enzyme activity. Excess ethanol also caused a reduction in mitochondrial membrane potential (MMP) and the quantity of reduced glutathione (GSH). Co-treatment of cells with ethanol and quercetin, catechin, caffeic acid and phytic acid significantly inhibited oxidative ethanol metabolism-induced cytotoxicity by blocking ROS production. When the cells were treated with ethanol after pretreatment of 4-methylpyrazole (4-MP), increased cytotoxicity, ROS production, antioxidant enzyme activity, and loss of MMP were observed. The addition of quercetin, catechin, caffeic acid and phytic acid to these cells showed suppression of non-oxidative ethanol metabolism-induced cytotoxicity, similar to oxidative ethanol metabolism. These results suggest that quercetin, catechin, caffeic acid and phytic acid have protective effects against ethanol metabolism-induced oxidative insult in SK-Hep-1 cells by blocking ROS production and elevating antioxidant potentials.

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“…The prevention by betaine feeding of both steatosis and inflammation observed in the ethanol fed rats could be the result of the prevention of oxidative stress by Betaine. This was shown in ethanol treated HepG2 cells where Betaine completely prevented oxidative stress in vitro (Oliva et al, 2011). …”

Section: Discussionmentioning

confidence: 82%

Betaine feeding prevents the blood alcohol cycle in rats fed alcohol continuously for 1month using the rat intragastric tube feeding model

Li¹,

Li²,

Caudill³

et al. 2011

Experimental and Molecular Pathology

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Background Blood alcohol levels (BAL) cycle up and down over a 7–8 day period when ethanol is fed continuously for one month in the intragastric tube feeding rat model (ITFRM) of alcoholic liver disease. The cycling phenomenon is due to an alternating increase and decrease in the metabolic rate. Recently, we found that S-adenosyl-methionine (SAMe) fed with alcohol prevented the BAL cycle. Method Using the ITFRM we fed rats betaine (2 g/kg/day) with ethanol for 1 month and recorded the daily 24 h urine ethanol level (UAL) to measure the BAL cycle. UAL is equivalent to BAL because of the constant ethanol infusion. Liver histology, steatosis and BAL were measured terminally after 1 month of treatment. Microarray analysis was done on the mRNA extracted from the liver to determine the effects of betaine and alcohol on changes in gene expression. Results Betaine fed with ethanol completely prevented the BAL cycle similar to SAMe. Betaine also significantly reduced the BAL compared to ethanol fed rats without betaine. This was also observed when SAMe was fed with ethanol. The mechanism involved in both cases is that SAMe is required for the conversion of epinephrine from norepinephrine by phenylethanolamine methyltransferase (PNMT). Epinephrine is 5 to 10 fold more potent than norepinephrine in increasing the metabolic rate. The increase in the metabolic rate generates NAD, permitting ADH to increase the oxidation of alcohol. NAD is the rate limiting factor in oxidation of alcohol by alcohol dehydrogenase (ADH). This explains how SAMe and betaine prevented the cycle. Microarray analysis showed that betaine feeding prevented the up regulation of a large number of genes including TLR2/4, Il-1b, Jax3, Sirt3, Fas, Ifngr1, Tgfgr2, Tnfrsf21, Lbp and Stat 3 which could explain how betaine prevented fatty liver. Conclusion Betaine feeding lowers the BAL and prevents the BAL cycle by increasing the metabolic rate. This increases the rate of ethanol elimination by generating NAD.

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Protective effect of quercetin, EGCG, catechin and betaine against oxidative stress induced by ethanol in vitro

Cited by 64 publications

References 42 publications

Quercetin protects mouse liver against CCl4-induced inflammation by the TLR2/4 and MAPK/NF-κB pathway

Quercetin protects mouse liver against CCl4-induced inflammation by the TLR2/4 and MAPK/NF-κB pathway

Potential in vitro Protective Effect of Quercetin, Catechin, Caffeic Acid and Phytic Acid against Ethanol-Induced Oxidative Stress in SK-Hep-1 Cells

Betaine feeding prevents the blood alcohol cycle in rats fed alcohol continuously for 1month using the rat intragastric tube feeding model

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