Protective effect of pumpkin seed extract against testicular toxicity induced by tramadol in adolescent and adult male albino rats: a light and electron microscopic study

Minisy, Fatma M; Massoud, Ahmed; Omara, Enayat A.; Metwally, Fatma Gouda; Hassan, Nabila S.

doi:10.4103/1687-4315.205828

Cited by 5 publications

(6 citation statements)

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“…Histopathological analysis of the testis supports the biochemical and molecular fi ndings. We found severe irregular changes in the testicular tissues of Tra groups, compared to the control group; these fi ndings are in line with previous studies (4,62,63). In general, the present fi ndings support Tra-induced toxicity in the testicular tissue.…”

supporting

confidence: 93%

Effects of tramadol administration on male reproductive toxicity in Wistar rats The role of oxidative stress, mitochondrial dysfunction, apoptosis-related gene expression, and nuclear factor kappa B signalling

Koohsari¹,

Ahangar²,

Mohammadi³

et al. 2020

BLL

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AIM: The present study investigated the role of redox balance, infl ammation, mitochondrial dysfunction, and apoptosis in Tramadol (Tra)-induced testicular toxicity. METHOD: Twenty-four male Wistar rats were randomly divided into either the control group or the groups receiving different doses of Tra (25, 50, and 75 mg/kg/day, i.p.) for 21 successive days. Testicular tissues were collected for oxidative stress, mitochondrial function, sperm assays and histopathological evaluation. Real-time polymerase chain reaction was performed to evaluate the markers of infl ammation and apoptosis. RESULTS: Tra caused a signifi cant reduction in the sperm count, motility and morphology, while it caused a marked increase in oxidative stress parameters. In addition, Tra induced testicular mitochondrial dysfunction due to the collapse of mitochondrial membrane potential and mitochondrial swelling. It also led to the signifi cant inhibition of anti-apoptotic Bcl-2 expression, besides a signifi cant increase in pro-apoptotic Bax expression. There was a signifi cant increase in the level of tumour necrosis factor-α, interlukin-1β and nuclear factor kappa B. Histopathological degenerative changes were observed in the testis after Tra exposure. CONCLUSIONS: The present results suggest that Tra exposure may lead to reproductive toxicity due to the loss of the antioxidant defence system, mitochondrial dysfunction, and activation of infl ammatory and apoptotic pathways (Tab. 4, Fig. 5, Ref. 63).

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supporting

confidence: 93%

Effects of tramadol administration on male reproductive toxicity in Wistar rats The role of oxidative stress, mitochondrial dysfunction, apoptosis-related gene expression, and nuclear factor kappa B signalling

Koohsari¹,

Ahangar²,

Mohammadi³

et al. 2020

BLL

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“…One of the main causes of male infertility is the exposure to opioid analgesic drugs that induce oxidative stress [4][5][6][7][8][9]. Tramadol is one of the opioid drugs that is extensively used as a pain-killer for chronic pain and cancers [10].…”

Section: Discussionmentioning

confidence: 99%

“…ere has been expanding global concerns on the decline of male fertility rates and testicular cancer in the past 3-5 decades [1,2]. Along with the conventional causes of male infertility [3], exposures to certain analgesic drugs such as opioid narcotics induce oxidative stress and adversely affect male fertility [4][5][6][7][8]. Opioid analgesic drugs are known to be the most effective medication for moderate to serious pain for adult and young patients [9].…”

Section: Introductionmentioning

confidence: 99%

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Pomegranate Seeds Extract Possesses a Protective Effect against Tramadol-Induced Testicular Toxicity in Experimental Rats

Minisy

Shawki

Omri

et al. 2020

BioMed Research International

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Tramadol is a centrally acting opioid analgesic that is extensively used. The chronic exposure to tramadol induces oxidative stress and toxicity especially for patients consuming it several times a day. Previously, we and others reported that tramadol induces testicular damage in rats. This study was conducted to investigate the possible protective effect of pomegranate seed extract (PgSE) against tramadol-induced testicular damage in adult and adolescent rats. Male rats were orally treated with tramadol or in a combination with PgSE for three weeks. Testes were then dissected and analyzed. Histological and ultrastructural examinations indicated that tramadol induced many structural changes in the testes of adult and adolescent rats including hemorrhage of blood vessels, intercellular spaces, interstitial vacuoles, exfoliation of germ cells in lumen, cell apoptosis, chromatin degeneration of elongated spermatids, and malformation of sperm axonemes. Interestingly, these abnormalities were not observed in tramadol/PgSE cotreated rats. The morphometric analysis revealed that tramadol disrupted collagen metabolism by elevating testicular levels of collagen fibers but that was protected in tramadol/PgSE cotreatment at both ages. In addition, DNA ploidy revealed that S phase of the cell cycle was diminished when adult and adolescent rats were treated with tramadol. However, the S phase had a normal cell population in the cotreated adult rats, but adolescent rats had a lower population than controls. Furthermore, the phytochemistry of PgSE revealed a high content of total polyphenols and total flavonoids within this extract; besides, the DPPH free radical scavenging activity was high. In conclusion, this study indicated that PgSE has a prophylactic effect against tramadol-induced testicular damage in both adult and adolescent ages, although the tramadol toxicity was higher in adolescent age to be completely protected. This prophylactic effect might be due to the high antioxidant compounds within the pomegranate seeds.

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“…Third group received oral dose of PSE (40 mg/kg body weight in distilled water (1 mL) and fourth group received daily oral dose of tramadol (20, 40, and 80 mg/kg body weight during the first, second, and third week) plus PSE (40 mg/ kg body weight). Owing to the antioxidant activity and free radical scavenging ability, PSE expressed prophylactic effect on tramadol-induced testicular damage which was comparatively less in the testes of the adolescent group than adult group (Minisy et al 2017).…”

Section: Protection Against Cellular Damagementioning

confidence: 99%

Pumpkin (Cucurbita pepo) Seed

Adsul¹,

Madkaikar

2020

Oilseeds: Health Attributes and Food Applications

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A pumpkin seed, also known as a "pepita", means "little seed of squash". Cucurbita pepo is the pumpkin species of the genus: Cucurbita, family: Cucurbits, sub-family: Cucurbitaceae, genera: Cucurbita L. Cucurbita pepo L. is the species among Cucurbitaceae family having the greatest monetary value of the genus. The seeds are typically rather flat, asymmetrically oval, light green in color, and may have a white outer hull. The Cucurbita pepo seeds have potential application and can be used as an alternative oil and protein source in novel food formulations such as cooking oils, as an ingredient in margarine blends, flours for instant soups, cookies, etc.

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Protective effect of pumpkin seed extract against testicular toxicity induced by tramadol in adolescent and adult male albino rats: a light and electron microscopic study

Cited by 5 publications

References 1 publication

Effects of tramadol administration on male reproductive toxicity in Wistar rats The role of oxidative stress, mitochondrial dysfunction, apoptosis-related gene expression, and nuclear factor kappa B signalling

Effects of tramadol administration on male reproductive toxicity in Wistar rats The role of oxidative stress, mitochondrial dysfunction, apoptosis-related gene expression, and nuclear factor kappa B signalling

Pomegranate Seeds Extract Possesses a Protective Effect against Tramadol-Induced Testicular Toxicity in Experimental Rats

Pumpkin (Cucurbita pepo) Seed

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