Protective effect of Pu-erh tea extracts against ethanol-induced gastric mucosal damage in rats

Yang, Jinna; Zhou, Wangyi; Gu, Yaru; Dai, Jinwei; Li, Xinxin; Tai, Ping; Li, Yanchuan; Ma, Xiaojing; Zhang, Yuyang

doi:10.3892/br.2018.1068

Cited by 10 publications

(9 citation statements)

References 53 publications

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“…An hour after ethanol treatment, rats were sacrificed under anesthesia after injection of sodium pentobarbital (60 mg/kg BW). The stomach was excised and cut open along the greater curvature, cleaned with cold normal saline and macroscopically evaluated for UI scoring as described previously (15). Each stomach was photographed for determination of UA percentage by ImageJ software (1.51j, National Institutes of Health, Bethesda, USA).…”

Section: Methodsmentioning

confidence: 99%

Pretreatment with Lactobacillus reuteri F-9-35 attenuates ethanol-induced gastric injury in rats

Sun¹,

Hou²,

Wang³

et al. 2018

Food & Nutrition Research

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BackgroundPrevious studies suggested that probiotics intervention may be one of the methods for preventing and/or treating gastric ulcer.ObjectiveThe aim of the study was to compare the preventive effects of a spaceflight mutant Lactobacillus reuteri F-9-35 and its wild type on ethanol-induced gastric injury in rats.DesignForty rats were randomly allocated into five groups: a normal group (NOR), ethanol group (EtOH), skim milk group (MILK), L. reuteri F-9-35 group (F935) and wild-type group (WT). The NOR and EtOH groups received 1 ml of distilled water by daily gavage for 14 days. The MILK group received 1 ml of skim milk alone, while the F935 and WT groups were administered 1 ml of skim milk containing the mutant and wild type (1 × 1010 colony-forming unit/ml) by daily gavage for 14 days, respectively. Acute gastric injury was induced by absolute alcohol 1 h after the final administration of different treatments, except for the NOR group.ResultsPretreatment with L. reuteri F-9-35, but not milk alone or milk with the L. reuteri wild type, showed significant reduction of ethanol-induced gastric injury, as evidenced by lowering of ulcer index, ulcer area (%), and histological lesion. F-9-35 decreased the levels of lipid peroxidation and myeloperoxidase and increased mucus, glutathione, and nitric oxide levels in gastric tissue. Moreover, F-9-35 inhibited the expression of proinflammatory genes including gastric tumor necrosis factor-α, interleukin-1β, and cyclooxygenase-2 and decreased the activity of nuclear factor kappa B (NF-κB).ConclusionThese findings indicated that L. reuteri F-9-35 pretreatment can attenuate ethanol-induced gastric injury in rats by inhibiting oxidative stress and inflammatory response. Together, L. reuteri F-9-35 has potential preventive efficacy on gastric ulcer.

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Section: Methodsmentioning

confidence: 99%

Pretreatment with Lactobacillus reuteri F-9-35 attenuates ethanol-induced gastric injury in rats

Sun¹,

Hou²,

Wang³

et al. 2018

Food & Nutrition Research

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“…Chronic alcohol consumption is one of the most important factors contributing to the high incidence and prevalence of gastric mucosa injury ( 3 ). Ethanol-induced gastric mucosal injury (acute or chronic), with the imbalance of protective and injury-inducing factors of the mucosa ( 4 ), maybe attenuated by the regulation of anti-inflammatory and antioxidant pathways ( 5 , 6 ). Reactive oxygen species (ROS) have previously been demonstrated have important roles in disease pathogenesis and to be involved in complex physiological processes associated with oxidative stress, such as cell signaling and apoptosis ( 7 – 9 ).…”

Section: Introductionmentioning

confidence: 99%

Theaflavins attenuate ethanol‑induced oxidative stress and cell apoptosis in gastric mucosa epithelial cells via downregulation of the mitogen‑activated protein kinase pathway

2018

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Ethanol-induced diseases of the gastric mucosa are the most common and refractory diseases of gastrointestinal system in clinic, and are mediated by oxidative stress and apoptosis pathways. Theaflavins (TFs) are considered to be antioxidants. The present study aimed to determine the molecular mechanism underlying the ability of TFs to attenuate ethanol-induced oxidative stress and apoptosis in GES-1 gastric mucosa epithelial cells. A Cell Counting Kit-8 (CCK-8) assay was performed to investigate the cell viability of GES-1 cells following administration of ethanol (0.5 mol/l) and subsequent treatment with TFs (20, 40 and 80 µg/ml) for specific time intervals. A carboxyfluorescein diacetate succinimidyl ester assay was used to measure proliferation and further investigate the results of the CCK-8 assay. Flow cytometry was performed to measure reactive oxygen species (ROS) levels and the apoptosis rates of GES-1 cells. Furthermore, levels of oxidative stress-associated factors, including malondialdehyde, superoxide dismutase and glutathione, were investigated using commercial kits. Reverse transcription-quantitative polymerase chain reaction and western blot assays were performed to determine the expression levels of apoptosis-associated factors, as well as the phosphorylation levels of extracellular signal-regulated kinase (ERK), c-Jun N-terminal kinase (JNK) and p38 kinase (p38). The results of the present study demonstrated that treatment with ethanol inhibited GES-1 cell proliferation, and enhanced ROS levels and apoptosis rates, potentially via downregulation of B-cell lymphoma-2 (Bcl-2) expression and upregulation of Bcl-2-associated X and caspase-3 expression levels, as well as enhancing the phosphorylation levels of ERK, JNK and p38. However, treatment with TFs was revealed to attenuate the effects of ethanol administration on GES-1 cells in a dose-dependent manner. In conclusion, TFs may attenuate ethanol-induced oxidative stress and apoptosis in gastric mucosa epithelial cells via downregulation of various mitogen-activated protein kinase pathways.

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“…In addition, tea has exhibited the neuro-protective effect, in terms of protecting against age-related neuro-degenerative disorders, depression and regulating the circadian clock [184][185][186][187][188][189][190]. Moreover, tea could inhibit gastric ulcer and improve gastrointestinal function [191][192][193][194][195]. Besides, tea could modulate gut microbiota composition (increase beneficial microorganisms and decrease harmful microorganisms), which might be beneficial to those in the risk of obesity, metabolic syndrome, hyperlipidemia, and cardiovascular diseases [196][197][198][199][200][201][202].…”

Section: Other Health Functionsmentioning

confidence: 99%

“…Ameliorate gastric ulcer, by decreasing the activity of myeloperoxidase and the concentration of asymmetric arginine in gastric mucosal homogenate. [194] Hetero-polysaccharides from green and black teas…”

Section: Potential Safety Issuesmentioning

confidence: 99%

Health Functions and Related Molecular Mechanisms of Tea Components: An Update Review

Tang

Meng

Gan

et al. 2019

IJMS

238

150

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Tea is widely consumed all over the world. Generally, tea is divided into six categories: White, green, yellow, oolong, black, and dark teas, based on the fermentation degree. Tea contains abundant phytochemicals, such as polyphenols, pigments, polysaccharides, alkaloids, free amino acids, and saponins. However, the bioavailability of tea phytochemicals is relatively low. Thus, some novel technologies like nanotechnology have been developed to improve the bioavailability of tea bioactive components and consequently enhance the bioactivity. So far, many studies have demonstrated that tea shows various health functions, such as antioxidant, anti-inflammatory, immuno-regulatory, anticancer, cardiovascular-protective, anti-diabetic, anti-obesity, and hepato-protective effects. Moreover, it is also considered that drinking tea is safe to humans, since reports about the severe adverse effects of tea consumption are rare. In order to provide a better understanding of tea and its health potential, this review summarizes and discusses recent literature on the bioactive components, bioavailability, health functions, and safety issues of tea, with special attention paid to the related molecular mechanisms of tea health functions.

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Protective effect of Pu-erh tea extracts against ethanol-induced gastric mucosal damage in rats

Cited by 10 publications

References 53 publications

Pretreatment with Lactobacillus reuteri F-9-35 attenuates ethanol-induced gastric injury in rats

Pretreatment with Lactobacillus reuteri F-9-35 attenuates ethanol-induced gastric injury in rats

Theaflavins attenuate ethanol‑induced oxidative stress and cell apoptosis in gastric mucosa epithelial cells via downregulation of the mitogen‑activated protein kinase pathway

Health Functions and Related Molecular Mechanisms of Tea Components: An Update Review

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