Protective effect of polysaccharides from Pholiota nameko on Lactobacillus casei ATCC 334 subjected to freeze-drying

Li, Haiping; Zhao, Ping; Zhang, Shuhai; Dengyun, Zhou; HeRong, Fu; Su, Yi; Wang, Xinqian

doi:10.1016/j.lwt.2019.108463

Cited by 8 publications

(5 citation statements)

References 21 publications

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“…On the other hand, low values of water activity were observed in the yacon juice freezedried samples: 0.311 ± 0.001 for L. sakei ACU-2 and 0.289 ± 0.015 for S. vitulinus ACU-10. High viability together with low water activity (< 0.6) are usually considered good quality indicators of freeze-dried cultures (Haiping et al, 2019). From a marketing perspective, the preservation of freeze-dried powder stability over the shelf-life is required.…”

Section: Yacon Juice As Cryoprotectantmentioning

confidence: 99%

Yacon juice as culture and cryoprotectant medium for Latilactobacillus sakei and Staphylococcus vitulinus autochthonous strains

Prpich

Sanabria

Gliemmo

et al. 2023

Braz. J. Food Technol.

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Yacon is mainly constituted of water and carbohydrates [single sugars and fructooligosaccharides (FOS)], thus being an excellent alternative for the growth and preservation of bacterial culture. Latilactobacillus sakei ACU-2 and Staphylococcus vitulinus ACU-10 comprised the autochthonous starter culture SAS-1 designed for the manufacture of dry sausages. This study evaluated the use of yacon juice as a potential growth medium and cryoprotectant for these bacteria. The growth medium was prepared with yacon juice supplemented with peptone and dipotassium phosphate. After growing, cells were resuspended in yacon juice (5, 10 and 25 mL/100 mL) and lyophilized. Viable cells were count before, immediately after lyophilization, and along 6 months of refrigerated storage. Both bacteria grew in every yacon concentration tested; however, juice concentration affected their growth. Latilactobacillus sakei grew at μ = 0.256 ± 0.01 giving the highest bacterial density at 10 mL/100 mL (Log DOmax 0.33 ± 0.01). While 5 mL/100 mL yacon juice provided the best conditions for S. vitulinus growth (μ = 0.215 ± 0.016; Log DOmax 0.32 ± 0.01). After lyophilization, the survival rate was 91.1% for L. sakei and 65.8% for S. vitulinus. Throughout storage, high cell counts suggested good stability of both bacteria. Results revealed that yacon juice comprises a nutritive substrate for the growth and cryopreservation of tested strains from the genus Latilactobacillus and Staphylococcus.

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Section: Yacon Juice As Cryoprotectantmentioning

confidence: 99%

Yacon juice as culture and cryoprotectant medium for Latilactobacillus sakei and Staphylococcus vitulinus autochthonous strains

Prpich

Sanabria

Gliemmo

et al. 2023

Braz. J. Food Technol.

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“…Microalgae are photosynthetic microorganisms capable of producing various metabolites, including lipids, proteins, carbohydrates, and pigments (Silva et al, 2016 ). Some combinations of protective agents, including skim milk, disaccharides (such as sucrose and trehalose), proteins (e.g., bovine serum albumin), and amino acids, have been tested on Lactobacillus strains for use as probiotics (Haiping et al, 2019 ).…”

Section: Introductionmentioning

confidence: 99%

Impact of Spirulina platensis biomass on the viability of Lactobacillus delbrueckii subsp. bulgaricus strain during the freeze-drying process

Ganchev

2024

bta

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In this work, we evaluated the protective capacity of Spirulina platensis biomass in preserving Lactobacillus delbrueckii subsp. bulgaricus WDCM 00102. The L. bulgaricus strain was freeze-dried in the presence of S. platensis biomass and the freeze-dried samples were then stored at 5 and 25°C for varying periods. Subsequently, the samples were rehydrated and bacterial plate counts were determined. The results indicate that a concentration of 12% S. platensis biomass was highly effective in preserving L. bulgaricus . Commercial products with higher S. platensis biomass content exhibited greater protective capacity. While S. platensis biomass is well-known for its prebiotic properties, its protective role has not been previously reported or thoroughly explored. This study demonstrates the protective capacity of S. platensis biomass in preserving L. bulgaricus , a strain particularly sensitive to preservation processes.

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“…Para asegurar altas tasas de supervivencia celular es necesario realizar una buena formulación que optimice la eficiencia del proceso, mediante la adecuada combinación de lioprotectores, concentración celular, temperatura y presión de vacío para obtener un producto seco con las características deseadas (Grauer et al, 2015;Haiping et al, 2019;Yang et al, 2022). Por lo anterior, el objetivo principal de este estudio fue evaluar el proceso de liofilización utilizando diferentes sustancias lioprotectoras y modelos bacterianos para usar como método de preservación en la CM-EM-UDEA.…”

Section: Introductionunclassified

Conservación de bacterias por liofilización en la Colección de Microorganismos CM-EM-UDEA, Medellín, Colombia

Quintero Rodríguez,

Montoya Arango,

Restrepo Posada

et al. 2023

Biota

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Las colecciones microbianas preservan cepas ex situ con el propósito de mantener los microorganismos viables, puros y estables en el tiempo. Para esto se recomienda preservar idealmente por criopreservación y liofilización. Actualmente, la Colección de Microorganismos de la Escuela de Microbiología de la Universidad de Antioquia (CM-EM-UDEA) preserva bacterias únicamente por criopreservación y requiere estandarizar la liofilización para que posibilite la viabilidad de los microorganismos por un mayor tiempo, optimice el almacenamiento y facilite el transporte manteniendo estables sus características. El objetivo fue evaluar el proceso de liofilización en diferentes bacterias de la CM-EM-UDEA. Se seleccionaron seis bacterias, se estandarizaron los inóculos y se usaron diferentes lioprotectores, evaluando la eficiencia de liofilización mediante viabilidad, pureza y estabilidad de cada microorganismo antes y después de liofilizar. En la mayoría de microorganismos el lioprotector que presentó mejores resultados de viabilidad y características físicas del producto seco fue la sacarosa, sin embargo, la efectividad observada en los otros lioprotectores frente a microorganismos específicos los convierte en una alternativa de uso de acuerdo a su disponibilidad. Este estudio permitió estandarizar el proceso de liofilización manteniendo 100 % de pureza, viabilidad y estabilidad en altos porcentajes, en la mayoría de los microorganismos evaluados.

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Protective effect of polysaccharides from Pholiota nameko on Lactobacillus casei ATCC 334 subjected to freeze-drying

Cited by 8 publications

References 21 publications

Yacon juice as culture and cryoprotectant medium for Latilactobacillus sakei and Staphylococcus vitulinus autochthonous strains

Yacon juice as culture and cryoprotectant medium for Latilactobacillus sakei and Staphylococcus vitulinus autochthonous strains

Impact of Spirulina platensis biomass on the viability of Lactobacillus delbrueckii subsp. bulgaricus strain during the freeze-drying process

Conservación de bacterias por liofilización en la Colección de Microorganismos CM-EM-UDEA, Medellín, Colombia

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