Protective Effect of Pogostone on 2,4,6-Trinitrobenzenesulfonic Acid-Induced Experimental Colitis via Inhibition of T Helper Cell

Abstract

Inflammatory bowel disease (IBD) is a chronic immune-related disease mainly caused by the disequilibrium of T helper (Th) cell paradigm? Pogostone (PO) is one of the major chemical constituents of Pogostemon cablin (Blanco) Benth. The present study aims to investigate the potential benefit of PO against IBD in a 2,4,6-trinitrobenzenesulfonic acid (TNBS)-induced experimental colitis model. PO treatment by enema significantly brought down the disease activity index (DAI) of the TNBS-challenged rats, which was manifested by the ameliorated inflammatory features including ulceration, adhesion, and edema. Hematoxylin-eosin (HE) staining and immunohistochemistry analysis showed that PO effectively relived colon damage by restoring epithelium, and more importantly, by inhibiting the infiltration of pro-inflammatory Th1 and Th17 cells in the colon. Additionally, PO inhibited the activity of myeloperoxidase and secretion of inflammatory cytokines including IFN-γ, IL-12p70, IL-17A, and IL-10. Together with our previous findings, the present data indicated that the anti-IBD effect of PO probably related to its direct inhibition on Th cell proliferation and suppression of the cytokines secretion. These results highlighted the potential of PO as a promising candidate to relieve IBD.

Polysaccharide from Pycnoporus sanguineus ameliorates dextran sulfate sodium‐induced colitis via helper T cells repertoire modulation and autophagy suppression

“…From Day 8~14, health status including body weight, food and water consumption, and disease activity index (DAI) were recorded daily. DAI was scored according to the criteria in Table 1 as described previously with mild modification (Mi et al, 2020; Su et al, 2017), that is DAI = (weight loss score + feces consistency score + hemafecia score) / 3. On the 15th day, all animals were bled from orbital plexus, and then sacrificed by cervical dislocation.…”

“…The colons were opened longitudinally. Macroscopic colonic inflammation was scored according to the criteria in Table 2 as described previously with mild modification (Luk, Ko, Fung, & Cho, 2002; Su et al, 2017). Finally, colons were segmented and stored appropriately for different purposes at once.…”

Indigo Naturalis Suppresses Colonic Oxidative Stress and Th1/Th17 Responses of DSS-Induced Colitis in Mice

“…CD4 + T cells mainly contain effector CD4 + T cells such as T helper 1 (Th1), Th2, Th17, and regulatory T cells (Tregs). Studies have shown that intestinal immune homeostasis depends on the regulation and balance of these CD4 + T cell subgroups, and the deregulated overexpansion and activation of Th1 and Th17 cells in relation to Tregs can lead to intestinal inflammation, such as IBD [10–12]. Since Th1 and Th17 cells could secrete large amounts of proinflammatory cytokines such as IFN- γ , TNF- α , IL-1 β , and IL-17, resulting in persistent intestinal inflammation [13], medications that can suppress colonic oxidative status and inhibit Th1/Th17 responses would be shown to be clinically effective.…”

Innovative, complementary and alternative therapy in inflammatory bowel diseases: A broad 2020s update

“…Pogostone (PO) is one of the major chemical constituents of Pogostemon cablin (Blanco) Benth. In a study by Su et al, PO improved the inflammatory state, reducing effects on the infiltration of total Th cells into the inflammatory colon, especially pro-inflammatory Th1, and pro-inflammatory cytokine levels (202).…”