Protective Effect of Pitao (<i>Pitavia punctata</i> (R. &amp; P.) Molina) Polyphenols against the Red Blood Cells Lipoperoxidation and the <i>In Vitro</i> LDL Oxidation

Castro, Ricardo I.; Forero-Doria, Oscar; Soto-Cerda, Luis; Peña‐Neira, Álvaro; Guzmán, Luís

doi:10.1155/2018/1049234

Cited by 6 publications

(5 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The FRAP assay was conducted according to Castro et al, (2018) [18] using the reagent 2,4,6-tris and (2-pyridyl)-s-triazine dissolved in 40 mM of HCl, 25 mL of 300 mM acetate buffer at pH 3.6, and 2.5 mL of 20 mM FeCl 3 ∗ 6 H 2 O. All samples were read at 593 nm using a spectrophotometer (Thermo Spectronic GENESYS 10UV).…”

Section: Methodsmentioning

confidence: 99%

In Silico and In Vitro Analysis of the 4,4′,4′′-((1,3,5-Triazine-2,4,6-triyl)tris(azanediyl))triphenol), an Antioxidant Agent with a Possible Anti-Inflammatory Function

Castro

Valenzuela-Riffo

Morales-Quintana

2019

BioMed Research International

Self Cite

View full text Add to dashboard Cite

Inflammation is a consequence of an array of biological reactions that occur in response to pain sensation, local injury, and cell damage. A large number of studies have demonstrated that quercetin and other flavonoids show anti-inflammatory effects; thus, in the present work, we evaluated a triazine-phenol derivative (TP derivative) compound as a possible drug candidate with anti-inflammatory activity. This compound was studied as a possible anti-inflammatory drug using synthesis and characterization by Fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), and mass spectrometry (MS). The derivative of melamine was evaluated for its antioxidant activity and exhibited good DPPH and FRAP antioxidant activity. Additionally, we evaluated the putative effect of the molecule on the COX-2 enzyme through molecular dynamic simulation (MDS), and the result suggested that the TP derivative is a potential anti-inflammatory agent that can interact with the COX-2 enzyme because of the high number of protein-ligand interactions observed with MDS. Finally, the study of theoretical physicochemical properties, the observation of low toxicity (hemolysis assay), and the evaluation of oral bioavailability of the TP derivative showed that it is a possible anti-inflammatory drug candidate.

show abstract

Section: Methodsmentioning

confidence: 99%

In Silico and In Vitro Analysis of the 4,4′,4′′-((1,3,5-Triazine-2,4,6-triyl)tris(azanediyl))triphenol), an Antioxidant Agent with a Possible Anti-Inflammatory Function

Castro

Valenzuela-Riffo

Morales-Quintana

2019

BioMed Research International

Self Cite

View full text Add to dashboard Cite

show abstract

“…Protecting the membrane integrity and inhibiting the oxidation of the LDL lipoprotein [130] Human RBCs H 2 O 2 Orchis latifolia and Centratherum anthelminticum extract…”

Section: Pitavia Punctata Extractmentioning

confidence: 99%

Reactive Oxygen Species and Antioxidant Interactions in Erythrocytes

Vani¹,

Pallavi²,

Choudhary³

et al. 2023

The Erythrocyte - A Unique Cell

View full text Add to dashboard Cite

There is a continuous generation of reactive oxygen species (ROS) in erythrocytes due to their microenvironment. Reactive oxygen species (ROS) and reactive nitrogen species are well known as both harmful and beneficial species. They help in activating the antioxidant enzymes. However, overproduction of ROS can cause fatal damage to cell structures, including lipids and membranes, proteins and cause oxidative stress. Erythrocytes have effective antioxidant defenses to maintain their structure and functions. They protect these cells from damage and maintain their activities. Studies have reported that antioxidant interventions in various situations have proved beneficial to erythrocytes. Therefore, they can be employed as in vitro models for antioxidant and free radical interactions and also are ideal cell models for translational studies.

show abstract

“…To determine the antioxidant capacity, methanol extracts were tested for their radical scavenging ability based on a 1,1-diphenyl-2-picrylhydrazyl (DPPH) discoloration assay [22] with the modification described to Castro et al (2018) [23]. The reaction mixtures were in triplicate and were measured at 517 nm in an Infinite ® 200 PRO NanoQuant spectrophotometer (BioTek Instruments, Inc., Winooski, VT, USA).…”

Section: Total Antioxidant Capacitymentioning

confidence: 99%

“…Additionally, the ferric-reducing antioxidant power (FRAP) analysis was performed according to Benzie and Strain (1996) [24] and modified by Castro et al, (2018) [23].…”

Section: Total Antioxidant Capacitymentioning

confidence: 99%

Evolution of the Volatile Organic Compounds, Phenols and Antioxidant Capacity during Fruit Ripening and Development of Rubus ulmifolius Schott Fruits

et al. 2022

Self Cite

View full text Add to dashboard Cite

Nowadays, a growing interest in consumers’ fruit with a high content of health-promoting compounds has been observed. In this sense, wild berries have received special attention based on their high accumulation of phenolic compounds, as well as their characteristic and pleasant aroma. In this work, we characterize the color development, antioxidant capacity, phenolic contents, and volatile profile of Rubus ulmifolius Schott fruit at different ripening stages during two seasons on the same orchard. Four stages were established based on the color parameter, which was consistent with changes in the weight and size of the fruit. In addition, total phenolic and flavonoid content showed a decrease during the fruit ripening, in contrast with the total anthocyanins content that increased at the final stages of ripening. In addition, the antioxidant capacity was evaluated through two approaches: FRAP and DPPH, which consistently displayed higher levels at the final stages in the two different seasons. Finally, the VOCs analysis showed an active synthesis of volatile compounds during the late stage of ripening, with alcohols being the most abundant compounds for each ripening stage. These results allow us to propose a classification of different ripening stages of the wild blackberry to have a better knowledge of this interesting fruit with higher healthy- and nutraceutical compounds.

show abstract

Protective Effect of Pitao (Pitavia punctata (R. & P.) Molina) Polyphenols against the Red Blood Cells Lipoperoxidation and the In Vitro LDL Oxidation

Cited by 6 publications

References 48 publications

In Silico and In Vitro Analysis of the 4,4′,4′′-((1,3,5-Triazine-2,4,6-triyl)tris(azanediyl))triphenol), an Antioxidant Agent with a Possible Anti-Inflammatory Function

In Silico and In Vitro Analysis of the 4,4′,4′′-((1,3,5-Triazine-2,4,6-triyl)tris(azanediyl))triphenol), an Antioxidant Agent with a Possible Anti-Inflammatory Function

Reactive Oxygen Species and Antioxidant Interactions in Erythrocytes

Evolution of the Volatile Organic Compounds, Phenols and Antioxidant Capacity during Fruit Ripening and Development of Rubus ulmifolius Schott Fruits

Contact Info

Product

Resources

About