Protective effect of Photobiomodulation Therapy and Bone Marrow Stromal Stem Cells Conditioned Media on Pheochromocytoma Cell Line 12 Against Oxidative Stress Induced by Hydrogen Peroxide

Abstract

Introduction: Bone marrow stromal stem cells (BMSCs), a type of adult stem cells, secrete bioactive molecules such as trophic factors, growth factors, chemokine and cytokines that may be effective against oxidative stress in neurodegenerative diseases. In this study, we examined the protective effect of BMSCs conditioned media (CM) and photobiomodulation therapy (PBMT) on PC12 cells exposed to H2O2 as an oxidative injury model. Methods: BMSCs were cultured and confirmed by flow cytometry analysis and underwent osteogenic and adipogenic differentiation. Then, PC12-H2O2 cells were co-treated with BMSCs-CM and PBMT. The effect of BMSCs-CM and PBMT (He-Ne laser, 632.8nm, 3mW, 1.2J/ cm2 , 378s) on Bax/Bcl2 expression, cell viability, was assessed by real-time PCR and MTT assay. The length of the Neurite and cell body areas were assessed by Cell A software. Results: Flowcytometry analysis, as well as osteogenic and adipogenic staining, confirmed the BMSCs. The length of the Neurite was the highest in the group which received CM+PBMT and cell body areas were significant in CM+PBMT compared to other groups. Based on our results, elevating H2O2 concentration increased cell death significantly and using concentrations of 250 µM resulted in a dramatic increase in the mortality compared to the other groups. Conclusion: Our result demonstrated that the combination of CM +PBMT has a protective effect on PC12 cells against oxidative stress.

Hydroxy-α-sanshool Possesses Protective Potentials on H2O2-Stimulated PC12 Cells by Suppression of Oxidative Stress-Induced Apoptosis through Regulation of PI3K/Akt Signal Pathway

“…There are a variety of antioxidant systems in cells, while the synergistic antioxidant effect is mainly achieved by eliminating intracellular ROS to prevent oxidative damage to the body [5]. In fact, oxidant/antioxidant levels are critical for neurodegeneration or neuroprotection, in which enzymes such as superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) constitute the key antioxidant defenses [6]. Excessive ROS not only is closely related to mitochondrial dysfunction but also can increase intracellular Ca 2+ concentration and activate some intracellular apoptotic pathways.…”

The Effect of bisphenol A and Photobiomodulation Therapy on Autophagy-Related Genes Induction in Adipose Tissue-Derived Stem Cells

“…20 The results of this study showed that PHT can increase the induction of autophagy in the ADSC and also cause the formation of more lipid droplets, which was consistent with the results of other studies. 21…”

Topical administration of Juglans regia L. leaf extract accelerates diabetic wound healing

“…Then, qRT-PCR reactions were performed for three biological replicates on the real-time PCR system (Applied Biosystems StepOne instrument) using SYBR Green Master Mix and sets of primers (Table 1 ). The final analyses were performed using the comparative CT method (2 −ΔΔct ) [ 13 , 34 ].…”