Protective effect of obestatin on indomethacin-induced acute gastric ulcer in rats: role of VEGF and TNF-α

Ibrahim, Reham M.; Allam, Mona M.; El-Gohary, Ola A; El-Talees, Alaa E.A; El-Hamady, Mohamed S

doi:10.4103/bmfj.bmfj_86_18

Cited by 9 publications

(9 citation statements)

References 2 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The present study revealed that IND significantly decreased NOx and this coincides with Adhikary et al, [37] El-Ashmawy et al, [32] and Ibrahim et al [6] who demonstrated that the decrease ulcers. [38,39] Therefore, the inhibitory activities of IND on PGE2 production, as well as the accumulation of free radicals, have been proposed as important biochemical processes in the development of peptic ulcers.…”

Section: Discussionsupporting

confidence: 91%

“…Because they disrupt gastroprotective factors, NSAIDs, particularly indomethacin (IND), are the most common cause of gastric lesion and ulceration. [5,6] Increased reactive radical production causes oxidative damage and inflammation of the stomach mucosa, leading to gastric lesions. [7] Free radicals oxidize mucosal barrier lipids and proteins, stimulate macrophages, and promote inflammatory cytokine production.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Rivastigmine ameliorates indomethacin experimentally induced gastric mucosal injury via activating α7nAChR with inhibiting oxidative stress and apoptosis

Khalaf

Ahmed

Welson

et al. 2022

J Biochem & Molecular Tox

View full text Add to dashboard Cite

The current study aimed to investigate the potential ameliorative role of Rivastigmine (RIVA), the anti‐Alzheimer drug, against the gastric mucosal injury caused by indomethacin (IND). The rats were divided into four groups: group I was given a vehicle as a control, group II was given RIVA (0.3 mg/kg) once daily intraperitoneal (ip) for 2 weeks, group III was given a single IP dose of 30 mg/kg IND, and group IV was given RIVA ip 2 weeks before the administration of IND. The gastric mucosal injury was detected by the estimation of ulcer index, gastric acidity, pepsin, and mucin concentrations. Malondialdehyde (MDA), superoxide dismutase (SOD), reduced glutathione (GSH), total nitrite/nitrate (NOx), and the expression of tumor necrosis factor‐α (TNF‐α), interleukin 6 (IL‐6), nuclear factor kappa B (NF‐κB), Hemoxygenase 1 (HO‐1), and caspase‐3 were all measured in gastric tissue. In addition, histological assessment and proliferating cell nuclear antigen (PCNA) immuno‐expression were studied. Gastric mucosal injury induced by IND was indicated by both biochemical and histopathological assessments. RIVA Pretreatment reduced ulcer index, MDA, TNF‐α, IL‐6, NF‐κB, and caspase‐3 and increased SOD, GSH, NOx, and HO‐1. RIVA improved the suppressed nuclear immunoreaction for PCNA observed with IND. The current findings provide novel evidence that RIVA possesses a prophylactic action against IND‐induced gastric mucosal damage in rats. Despite being a cholinergic drug that is associated with increased pepsin and stomach acidity, RIVA protected against IND‐induced gastric mucosal injury via activating α7nAChR and inhibiting oxidative stress and apoptosis.

show abstract

Section: Discussionsupporting

confidence: 91%

mentioning

confidence: 99%

Rivastigmine ameliorates indomethacin experimentally induced gastric mucosal injury via activating α7nAChR with inhibiting oxidative stress and apoptosis

Khalaf

Ahmed

Welson

et al. 2022

J Biochem & Molecular Tox

View full text Add to dashboard Cite

show abstract

“…Our findings corroborate previous work by Alkushi and Elsawy (27) who also reported increased gastric acid volume, ulcer index, and gastric pH in indomethacin-treated rats. Additionally, they found that quercetin treatment showed a marked decrease in the indomethacin-induced gastric damage suggesting antioxidant and gastroprotective effects (28). Similar results were reported by Ibrahim et al, (28).Our findings on the anti-inflammatory activity of melatonin corroborates previous work by Cuesta et al, (29) in which they reported that the administration of exogenous melatonin in old mice significantly lowered the expression of TNF-α, IL-1β, and IL-6 and increased the expression of IL-10 (29).…”

Section: Discussionmentioning

confidence: 98%

“…Additionally, they found that quercetin treatment showed a marked decrease in the indomethacin-induced gastric damage suggesting antioxidant and gastroprotective effects (28). Similar results were reported by Ibrahim et al, (28).Our findings on the anti-inflammatory activity of melatonin corroborates previous work by Cuesta et al, (29) in which they reported that the administration of exogenous melatonin in old mice significantly lowered the expression of TNF-α, IL-1β, and IL-6 and increased the expression of IL-10 (29). However, Konturek et al, (30) concluded that the gastroprotective effect of melatonin occurs due to the activation of the COX-prostaglandin E (2) (PGE-2) system.…”

Section: Discussionmentioning

confidence: 98%

Protective Effects of Quercetin and Melatonin on Indomethacin Induced Gastric Ulcers in Rats

et al. 2020

View full text Add to dashboard Cite

Background: Medications to prevent the development of NSAID-induced gastric ulcers have a large range of unpleasant side effects. Recent efforts have been focused on determining safer alternative nontoxic and natural forms of anti-ulcer treatments. Methods: Twenty-four male rats were divided into 4 groups: 1: control group that received no treatment; 2: the indomethacin-treated group that received 20 mg/kg of indomethacin for 2 days to induce the development of gastric ulcers; 3: quercetin-treated group that in addition to the indomethacin treatment, received 50 mg/kg of quercetin 6 hours after and then daily for 14 days and; 4: the melatonin-treated group which received 20 mg/kg of melatonin 6 hours after each indomethacin treatment and then daily for 14 days. All drugs were administered orally. The following parameters were assessed in each group: mean ulcer index of gastric tissue, gastric acid volume and pH, oxidative stress markers: malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GSH), inflammatory markers: PGE-2, TNF-α, and IL-10, nitric oxide (NO) levels and the relative gene expression of BAX, BCL-2 and COX-2 by real time PCR. Results: Our findings revealed that the indomethacin-treated group had a significantly increased (p< 0.05) ulcer index, gastric acid volume, and elevated levels of stress, inflammatory, and apoptotic markers compared to controls. In the groups that received quercetin or melatonin, these factors were all significantly decreased (p< 0.05). Between quercetin and melatonin, there was no significant difference in their gastroprotective effect. Conclusions: Both quercetin and melatonin had protective antioxidant, anti-inflammatory and antiapoptotic activity against indomethacin-induced gastric ulcers.

show abstract

“…Presence of congested blood vessels in the lamina propria of gastric mucosa was explained by Ibrahim et al, 2018 [39] who mentioned that NSAIDs exert direct toxic effect of on the wall of small blood vessels leading to ischemia followed by vasodilatation and extravasations of blood from their luminae. The same authors added that another factor might be implicated is the destructive effect of the drug on the mucosal barrier and intercellular integrity exposing the capillaries and venules to the harmful effects of hydrochloric acid and gastric secretions.…”

Section: Discussionmentioning

confidence: 99%

The Possible Protective Effect of Ocimum Basilicum L. Aqueous Extract on Aspirin Induced Damage of Gastric Mucosa in Adult Male Albino Rats: Histological and Ultrastructural Study

Abdelfadeel

Alazouny

2019

Journal of Medical Histology

View full text Add to dashboard Cite

Background and Objectives: One of the new strategies to limit the gastric mucosal injury induced by aspirin is adding antioxidant. Therefore, our aim was to detect histological changes that may occur in fundic mucosa of adult male albino rats given aspirin and the protective role of Ocimum Basillicum administration. Materials and Methods: Thirty adult male albino rats were classified into three groups: Group I (control group, which were subdivided into two equal subgroups, Group II (aspirin treated group, given aspirin in a dose of 12.5 mg/kg by orogastric tube for 4 weeks) and Group III (coadministration group, given aspirin in the same previous dose in concomitant with Basil in a dose of 200 mg/Kg b.w by orogastric tube). Results: Fundic mucosa of aspirin treated group showed disorganized fundic glands, desquamation of surface epithelial cells, mononuclear cell infiltration and congested blood vessels. Many parietal cells exhibited small nuclei with increased amount of heterochromatin, dilated canaliculi containing few short microvilli and vacuole-like structures. Many eosinophils were seen in lamina propria. Many chief cells appeared with shrunken pyknotic nuclei and vacuolated cytoplasm. Statistically significant increase in the mean area percent of collagen fibers and decrease in the mean thickness of mucous film in PAS stained sections of fundic mucosa of aspirin treated group. These changes were reduced in the coadministration group. Conclusion: Ocimum Basilicum aqueous extract has beneficial protective effects against the deleterious effects of aspirin on the fundic mucosa. Therefore, it may be a suitable nutritional supplement or therapeutic drug to protect against aspirin-induced gastric damage.

show abstract

Protective effect of obestatin on indomethacin-induced acute gastric ulcer in rats: role of VEGF and TNF-α

Cited by 9 publications

References 2 publications

Rivastigmine ameliorates indomethacin experimentally induced gastric mucosal injury via activating α7nAChR with inhibiting oxidative stress and apoptosis

Rivastigmine ameliorates indomethacin experimentally induced gastric mucosal injury via activating α7nAChR with inhibiting oxidative stress and apoptosis

Protective Effects of Quercetin and Melatonin on Indomethacin Induced Gastric Ulcers in Rats

The Possible Protective Effect of Ocimum Basilicum L. Aqueous Extract on Aspirin Induced Damage of Gastric Mucosa in Adult Male Albino Rats: Histological and Ultrastructural Study

Contact Info

Product

Resources

About