Protective effect of Neorhodomela aculeata methanolic extract through the suppressive action on NF-κB and STAT pathway in IL-1β and IFN-γ induced β-cell damage

Abstract

The protective effects of Neorhodomela aculeata methanolic extract (N. aculeata) on IL-1β and IFN-γ induced β-cell cytotoxicity were examined in a rat insulinoma (RIN) cell line and islets. In the cytokine-treated RIN cells, nitric oxide (NO) production was reduced about 94% at 50 μg/ml of N. aculeata and its suppressive effect was ten times greater as compared to other effective oriental herbs, such as C. cinnamoni and A. capillaries. The inhibitory effects of NO production occurred through the suppression of iNOS (inducible nitric oxide synthase) gene expression which has been confirmed with the changes of iNOS mRNA and protein levels. N. aculeata attenuated the cytokine-induced increase in nuclear factor-kappa B (NF-κB) binding activity, p50 and p65 subunit levels in their nuclei, and at inhibitory kappa B alpha degradation in the cytosol. N. aculeata also attenuated the levels of phosphorylated signal transducer and activator of transcription (STAT)-1 and -3 in whole RIN cells as well as the nuclear translocation of the STAT proteins. Therefore, the cytoprotective effects of N. aculeata were possibly mediated through the suppression of NF-κB and STAT pathways. These findings suggest the beneficial effects of N. aculeata when used for the prevention or attenuation of inflammatory and Type 1 diabetogenic processes.

Potential Targets for Anti-Inflammatory and Anti-Allergic Activities of Marine Algae: An Overview

“…Also, sulfated polysaccharides obtained from brown algae Fucus vesiculosus, Laminaria japonica, and Sargassum hemiphyllum are effective inactivators of NF-B as evidenced by Park et al 2011 [28], Li et al 2011 [73], and Hwang et al 2011 [32]. On the other hand, red alga Neorhodomela aculeate was shown to attenuate the cytokine-induced increase in NF-B binding activity, p50 and p65 subunit levels in their nuclei, and I Bdegradation in the cytosol [74]. Likewise, a new diterpene extracted from the red alga Laurencia glandulifera, Neorogioltriol, exhibited inhibitory effect on luciferase activity in LPS-stimulated RAW 264.7 cells, stably transfected with the NF-B-dependent luciferase reporter.…”