Protective Effect of Neferine in Permanent Cerebral Ischemic Rats via Anti-Oxidative and Anti-Apoptotic Mechanisms

Sengking, Jirakhamon; Oka, Chio; Yawoot, Nuttapong; Tocharus, Jiraporn; Chaichompoo, Waraluck; Suksamrarn, Apichart; Tocharus, Chainarong

doi:10.1007/s12640-022-00568-6

Cited by 6 publications

(3 citation statements)

References 55 publications

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“…So, an extract of African walnut, or Tetracarpidium conophorum , is recently shown to improve the PD-like signs in rats, probably via Hsp70 modulation ( Tokunbo et al, 2023 ). Neferine, derived from seeds of lotus plants, seems to increase neuronal tolerance to ischemia via Hsp70-induction machinery ( Sengking et al, 2022 ).…”

Section: Hsp70 As a Target To Treat Neurodegenerationmentioning

confidence: 99%

Closest horizons of Hsp70 engagement to manage neurodegeneration

Venediktov,

Bushueva,

Kudryavtseva

et al. 2023

Front. Mol. Neurosci.

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Our review seeks to elucidate the current state-of-the-art in studies of 70-kilodalton-weighed heat shock proteins (Hsp70) in neurodegenerative diseases (NDs). The family has already been shown to play a crucial role in pathological aggregation for a wide spectrum of brain pathologies. However, a slender boundary between a big body of fundamental data and its implementation has only recently been crossed. Currently, we are witnessing an anticipated advancement in the domain with dozens of studies published every month. In this review, we briefly summarize scattered results regarding the role of Hsp70 in the most common NDs including Alzheimer’s disease (AD), Parkinson’s disease (PD), and amyotrophic lateral sclerosis (ALS). We also bridge translational studies and clinical trials to portray the output for medical practice. Available options to regulate Hsp70 activity in NDs are outlined, too.

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Section: Hsp70 As a Target To Treat Neurodegenerationmentioning

confidence: 99%

Closest horizons of Hsp70 engagement to manage neurodegeneration

Venediktov,

Bushueva,

Kudryavtseva

et al. 2023

Front. Mol. Neurosci.

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show abstract

“…Nef exhibits diverse pharmacological activities, including neuroprotection, anti-platelet aggregation, vasodilation, blood pressure reduction, antioxidant [1][2][3] . Previous research shows that Nef modulated neurone Nrf2 signalling to protect mitochondrial 4 , diminishing oxidative stress and apoptosis to exert its neuroprotective effect 5 . Research of Nef in IS were focused on neuroprotective effects, the mechanisms of Nef protective effects on BBB remains unclear.…”

Section: Introductionmentioning

confidence: 99%

Neferine inhibits BMECs pyroptosis and maintains blood-brain barrier integrity in ischemic stroke by triggering a cascade reaction of PGC-1α

Zheng,

Zhu,

Qiu

et al. 2024

Preprint

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Blood-brain barrier disruption is a critical pathological event in the progression of ischemic stroke (IS). Most studies regarding the therapeutic potential of neferine (Nef) on IS have focused on neuroprotective effect. However, whether Nef attenuates BBB disruption during IS is unclear. We here used mice underwent transient middle cerebral artery occlusion (tMCAO) in vivo and bEnd.3 cells exposed to oxygen-glucose deprivation/reoxygenation (OGD/R) injury in vitro to simulate cerebral ischemia. We showed that Nef reduced neurobehavioral dysfunction and protected brain microvascular endothelial cells and BBB integrity. Molecular docking, short interfering (Si) RNA and plasmid transfection results showed us that PGC-1α was the most binding affinity of biological activity protein for Nef. And verification experiments were showed that Nef upregulated PGC-1α expression to reduce mitochondrial oxidative stress and promote TJ proteins expression, further improves the integrity of BBB in mice. Intriguingly, our study showed that neferine is a natural PGC-1α activator and illustrated the mechanism of specific binding site. Furthermore, we have demonstrated Nef reduced mitochondria oxidative damage and ameliorates endothelial inflammation by inhibiting pyroptosis to improve BBB permeability through triggering a cascade reaction of PGC-1α via regulation of PGC-1α/NLRP3/GSDMD signaling pathway to maintain the integrity of BBB in ischemia/reperfusion injury.

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“…Nef exhibits diverse pharmacological activities: including neuroprotection: anti-platelet aggregation: vasodilation: blood pressure reduction: antioxidant 8 – 10 . Previous research shows that Nef modulated neurone Nrf2 signalling to protect mitochondrial 11 : diminishing oxidative stress and apoptosis to exert its neuroprotective effect 12 . Research of Nef in IS were focused on neuroprotective effects: the mechanisms of Nef protective effects on BBB remains unclear.…”

Section: Introductionmentioning

confidence: 99%

Neferine inhibits BMECs pyroptosis and maintains blood–brain barrier integrity in ischemic stroke by triggering a cascade reaction of PGC-1α

Zheng,

Zhu,

Qiu

et al. 2024

Sci Rep

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Blood–brain barrier disruption is a critical pathological event in the progression of ischemic stroke (IS). Most studies regarding the therapeutic potential of neferine (Nef) on IS have focused on neuroprotective effect. However, whether Nef attenuates BBB disruption during IS is unclear. We here used mice underwent transient middle cerebral artery occlusion (tMCAO) in vivo and bEnd.3 cells exposed to oxygen–glucose deprivation/reoxygenation (OGD/R) injury in vitro to simulate cerebral ischemia. We showed that Nef reduced neurobehavioral dysfunction and protected brain microvascular endothelial cells and BBB integrity. Molecular docking, short interfering (Si) RNA and plasmid transfection results showed us that PGC-1α was the most binding affinity of biological activity protein for Nef. And verification experiments were showed that Nef upregulated PGC-1α expression to reduce mitochondrial oxidative stress and promote TJ proteins expression, further improves the integrity of BBB in mice. Intriguingly, our study showed that neferine is a natural PGC-1α activator and illustrated the mechanism of specific binding site. Furthermore, we have demonstrated Nef reduced mitochondria oxidative damage and ameliorates endothelial inflammation by inhibiting pyroptosis to improve BBB permeability through triggering a cascade reaction of PGC-1α via regulation of PGC-1α/NLRP3/GSDMD signaling pathway to maintain the integrity of BBB in ischemia/reperfusion injury.

show abstract

Protective Effect of Neferine in Permanent Cerebral Ischemic Rats via Anti-Oxidative and Anti-Apoptotic Mechanisms

Cited by 6 publications

References 55 publications

Closest horizons of Hsp70 engagement to manage neurodegeneration

Closest horizons of Hsp70 engagement to manage neurodegeneration

Neferine inhibits BMECs pyroptosis and maintains blood-brain barrier integrity in ischemic stroke by triggering a cascade reaction of PGC-1α

Neferine inhibits BMECs pyroptosis and maintains blood–brain barrier integrity in ischemic stroke by triggering a cascade reaction of PGC-1α

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